Magnetic pH-responsive nanogels as multifunctional delivery tools for small interfering RNA (siRNA) molecules and iron oxide nanoparticles (IONPs)

Curcio, Alberto; Riedinger, Andreas; Palumberi, Domenico; Falqui, Andrea; Pellegrino, Teresa

doi:10.1039/c2cc17223b

Cited by 54 publications

(27 citation statements)

References 12 publications

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“…Thus, both MNPs and MNGs can be very interesting for both diagnostic and therapeutic applications in the biomedical field. [2][3][4][5][6][7] Specifically, in the area of therapy, by using an external magnetic field, MNPs and MNGs that are loaded with a chemo/radio-agent can be magnetically guided in a controlled way through the cardiovascular system to the area of interest, thus enabling selective delivery to pathological tissues/organs. In another therapeutic application, MNPs and MNGs can induce a temperature increase in the targeted zone under the application of an ac-magnetic field for hyperthermia purposes.…”

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confidence: 99%

Synthesis and characterization of PDEAEMA‐based magneto‐nanogels: Preliminary results on the biocompatibility with cells of human peripheral blood

Pikabea

Ramos

Papachristos

et al. 2015

J. Polym. Sci. Part A: Polym. Chem.

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Nanogels based on biocompatible, dual pH-and temperature-sensitive poly(2-(diethylamino)ethyl) methacrylate (PDEAEMA) have been successfully used as nanocontainers for the encapsulation of magnetite, Fe 3 O 4 magnetic nanoparticles (MNPs). For this purpose, citric acid-coated MNPs were encapsulated into previously synthesized PDEAEMA-based nanogels using a poly(ethyleneglycol)-based stabilizer. After the encapsulation of the magnetite MNPs, the so-called magneto-nanogels (MNGs) were proved to be multiresponsive on temperature, pH, and magnetic field and colloidally stable. Moreover, preliminary studies on the biocompatibility of these MNGs with cells of human peripheral blood were performed and evidenced quite tolerable biocompatibility, thus suggesting potential use in biomedical applications.

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mentioning

confidence: 99%

Synthesis and characterization of PDEAEMA‐based magneto‐nanogels: Preliminary results on the biocompatibility with cells of human peripheral blood

Pikabea

Ramos

Papachristos

et al. 2015

J. Polym. Sci. Part A: Polym. Chem.

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“…Following these aims, several research groups have successfully investigated various aspects of interactions between functional nanoparticulate systems and cells. [6][7][8][9][10][11][12][13][14] For example, Brust and co-workers shed light on the intracellular fate of spherical gold nanoparticles coated with cell penetrating peptides and suggested ways of pre-programming nanoparticle migration within the cytoplasm. 15,16 Alternatively Mirkin and colleagues performed a systematic study on the internalization rate of DNA modified gold nanoparticles and suggested new directions for targeted gene therapy.…”

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confidence: 99%

Exocytosis of peptide functionalized gold nanoparticles in endothelial cells

et al. 2012

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We present the exocytosis profile of two types of peptide-coated nanoparticles, which have similar charge and size but different functionality. While one kind of particles appears to progressively exocytose, the other one has a more complex profile, suggesting that some of the particles are re-uptaken by the cells. Both types of particles retain their colloidal stability after exocytosis.Understanding the interactions of functional nanoparticles with biological cells is of tremendous importance not only for new developments in sensing, imaging and therapy but also for realizing the fundamental cellular mechanisms and cytotoxicity of nanomaterials.1-5 With an accumulated knowledge of how the size, shape and functionality of nanomaterials influence their fate in cells, one should be able to synthesize nanoparticles on demand, with appropriate functionality and which are best suited to a particular application. This means that particles would be designed to (a) be taken up by the cell in desirable numbers, (b) deliberately escape from the endosomes, (c) access the cytoplasm and migrate selectively through the cell for a given time, (d) target specific compartments and organelles (i.e. nucleus, mitochondria) to perform desired tasks and (e) exocytose in high numbers leaving the cell intact. Following these aims, several research groups have successfully investigated various aspects of interactions between functional nanoparticulate systems and cells.6-14 For example, Brust and co-workers shed light on the intracellular fate of spherical gold nanoparticles coated with cell penetrating peptides and suggested ways of pre-programming nanoparticle migration within the cytoplasm.15,16 Alternatively Mirkin and colleagues performed a systematic study on the internalization rate of DNA modified gold nanoparticles and suggested new directions for targeted gene therapy.17,18 Our group and others have investigated how the size, shape and charge of nanoparticles influence their cellular uptake, [19][20][21][22][23] and how the number of endocytosed particles correlates with the nanoparticles' heating efficiency during laser hyperthermia. 20,24Despite the extensive work on gold nanoparticle uptake and intracellular fate, 7,25 a very limited number of papers have discussed the exocytosis of functional gold nanoparticles. 26 Since gold is one of the most promising candidates for biomedical applications in the near future, exocytosis studies are of great importance, because they are directly correlated with chronic cytotoxicity and nanoparticle intracellular retention times.In this paper we investigated the cellular uptake and exocytosis of two types of peptide functionalized gold nanospheres by an important category of mammalian cells, namely human endothelial cells (HUVECs). The specific types of particles were chosen due to their ease of preparation and particular function. To keep our studies consistent, both types of nanoparticles were designed to have similar hydrodynamic size and charge. The first batch of nanoparticles ('inh...

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“…Second, SPION can infiltrate into hydrogels during swelling. In a previous study, SPIONs with a size of 7 nm were prepared by a thermal decomposition method and siRNAs were encapsulated via pH-responsive volume transition of nanogels [63]. To create pH-responsive polymeric hydrogels, monomers including 2-vinyl pyridine (2-VP) and divinylbenzene (DVB) were polymerized by addition of azobis(isobutyl-amidine hydrochloride) (AIBA) initiators.…”

Section: Formulations Of Spion-based Carriersmentioning

confidence: 99%

Superparamagnetic iron oxide nanoparticle-based delivery systems for biotherapeutics

Mok

Zhang

2012

Expert Opinion on Drug Delivery

120

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Introduction Superparamagnetic iron oxide nanoparticle (SPION)-based carrier systems have many advantages over other nanoparticle-based systems. They are biocompatible, biodegradable, facilely tunable, and superparamagnetic and thus controllable by an external magnetic field. These attributes enable their broad biomedical applications. In particular, magnetically-driven carriers are drawing considerable interest as an emerging therapeutic delivery system because of their superior delivery efficiency. Area covered This article reviews the recent advances in use of SPION-based carrier systems to improve the delivery efficiency and target specificity of biotherapeutics. We examine various formulations of SPION-based delivery systems, including SPION micelles, clusters, hydrogels, liposomes, and micro/nanospheres, as well as their specific applications in delivery of biotherapeutics. Expert opinion Recently, biotherapeutics including therapeutic cells, proteins and genes have been studied as alternative treatments to various diseases. Despite the advantages of high target specificity and low adverse effects, clinical translation of biotherapeutics has been hindered by the poor stability and low delivery efficiency compared to chemical drugs. Accordingly, biotherapeutic delivery systems that can overcome these limitations are actively pursued. SPION-based materials can be ideal candidates for developing such delivery systems because of their excellent biocompatibility and superparamagnetism that enables long-term accumulation/retention at target sites by utilization of a suitable magnet. In addition, synthesis technologies for production of finely-tuned, homogeneous SPIONs have been well developed, which may promise their rapid clinical translation.

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Magnetic pH-responsive nanogels as multifunctional delivery tools for small interfering RNA (siRNA) molecules and iron oxide nanoparticles (IONPs)

Cited by 54 publications

References 12 publications

Synthesis and characterization of PDEAEMA‐based magneto‐nanogels: Preliminary results on the biocompatibility with cells of human peripheral blood

Synthesis and characterization of PDEAEMA‐based magneto‐nanogels: Preliminary results on the biocompatibility with cells of human peripheral blood

Exocytosis of peptide functionalized gold nanoparticles in endothelial cells

Superparamagnetic iron oxide nanoparticle-based delivery systems for biotherapeutics

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