Magnetic Particles for CTC Enrichment

Liu, Peng; Jonkheijm, Pascal; Terstappen, Leon W.M.M.; Stevens, Michiel

doi:10.3390/cancers12123525

Cited by 28 publications

(15 citation statements)

References 127 publications

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“…If the size of the NPs is less than 20 nm, then after removal of the external field, due to strong thermal fluctuations magnetic nanoparticles display superparamagnetic behaviour, which possesses no residual magnetization in absence of a magnetic field. Whereas, if the size of the NPs is higher than this limit then some amount of residual magnetization is present in them and they are classified as ferromagnetic NPs [ 55 , 61 , 62 ].…”

Section: Nanotechnologymentioning

confidence: 99%

Isolation, Detection and Analysis of Circulating Tumour Cells: A Nanotechnological Bioscope

et al. 2023

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Cancer is one of the dreaded diseases to which a sizeable proportion of the population succumbs every year. Despite the tremendous growth of the health sector, spanning diagnostics to treatment, early diagnosis is still in its infancy. In this regard, circulating tumour cells (CTCs) have of late grabbed the attention of researchers in the detection of metastasis and there has been a huge surge in the surrounding research activities. Acting as a biomarker, CTCs prove beneficial in a variety of aspects. Nanomaterial-based strategies have been devised to have a tremendous impact on the early and rapid examination of tumor cells. This review provides a panoramic overview of the different nanotechnological methodologies employed along with the pharmaceutical purview of cancer. Initiating from fundamentals, the recent nanotechnological developments toward the detection, isolation, and analysis of CTCs are comprehensively delineated. The review also includes state-of-the-art implementations of nanotechnological advances in the enumeration of CTCs, along with future challenges and recommendations thereof.

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Section: Nanotechnologymentioning

confidence: 99%

Isolation, Detection and Analysis of Circulating Tumour Cells: A Nanotechnological Bioscope

et al. 2023

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“…Diagnostic tools to detect and isolate CTCs have been developed [ 16 , 17 ]. These include immunocytological approaches (immunocytochemistry, CellSearch system, flow cytometry), RNA-based molecular technologies (qPCR, FISH), as well as functional assays [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Removal of Circulating Tumor Cells from Blood Samples of Cancer Patients Using Highly Magnetic Nanoparticles: A Translational Research Project

et al. 2022

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The count of circulating tumor cells (CTCs) has been associated with a worse prognosis in different types of cancer. Perioperatively, CTCs detach due to mechanical forces. Diagnostic tools exist to detect and isolate CTCs, but no therapeutic technique is currently available to remove CTCs in vivo from unprocessed blood. The aim of this study was to design and test new magnetic nanoparticles to purify whole blood from CTCs. Novel magnetic carbon-coated cobalt (C/Co) nanoparticles conjugated with anti-epithelial cell adhesion molecule (EpCAM) antibodies were synthesized, and their antifouling and separation properties were determined. The newly developed C/Co nanoparticles showed excellent separation and antifouling properties. They efficiently removed tumor cells that were added to healthy subjects’ blood samples, through an anti-EpCAM antibody interaction. The nanoparticles did not interact with other blood components, such as lymphocytes or the coagulation system. In blood samples of carcinoma patients suffering from metastatic disease, on average, ≥68% of CTCs were removed. These nanoparticles could prompt the development of a blood purification technology, such as a dialysis-like device, to perioperatively remove CTCs from the blood of cancer patients in vivo and potentially improve their prognosis.

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“…Microparticle concentration realized by efficient fluidic methods has become an important research area. Many techniques based on various principles have been demonstrated to achieve particle concentration, such as magnetic [ 8 , 9 ], acoustic [ 10 , 11 , 12 ], optical [ 13 ], and dielectrophoretic [ 14 ] methods.…”

Section: Introductionmentioning

confidence: 99%

Concentration of Microparticles Using Flexural Acoustic Wave in Sessile Droplets

Peng

Zhou

et al. 2022

Sensors

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Acoustic manipulation of microparticles and cells has attracted growing interest in biomedical applications. In particular, the use of acoustic waves to concentrate particles plays an important role in enhancing the detection process by biosensors. Here, we demonstrated microparticle concentration within sessile droplets placed on the hydrophobic surface using the flexural wave. The design benefits from streaming flow induced by the Lamb wave propagated in the glass waveguide to manipulate particles in the droplets. Microparticles will be concentrated at the central area of the droplet adhesion plane based on the balance among the streaming drag force, gravity, and buoyancy at the operating frequency. We experimentally demonstrated the concentration of particles of various sizes and tumor cells. Using numerical simulation, we predicted the acoustic pressure and streaming flow pattern within the droplet and characterized the underlying physical mechanisms for particle motion. The design is more suitable for micron-sized particle preparation, and it can be valuable for various biological, chemical, and medical applications.

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Magnetic Particles for CTC Enrichment

Cited by 28 publications

References 127 publications

Isolation, Detection and Analysis of Circulating Tumour Cells: A Nanotechnological Bioscope

Isolation, Detection and Analysis of Circulating Tumour Cells: A Nanotechnological Bioscope

Removal of Circulating Tumor Cells from Blood Samples of Cancer Patients Using Highly Magnetic Nanoparticles: A Translational Research Project

Concentration of Microparticles Using Flexural Acoustic Wave in Sessile Droplets

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