Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum

Jin, Hui; Lin, Jin‐Ming; Wang, Xu; Xin, Tian-Bing; Liang, Shu-Xuan; Li, Zhenjia; Hu, GuoMao

doi:10.1016/j.jpba.2009.06.011

Cited by 37 publications

(16 citation statements)

References 32 publications

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“…Also, the linear concentration range of the microsensor based on TEX/DP covers the normal range of f‐L‐T4 in serum and the linear concentration range of the stochastic microsensor HD/DP includes the normal range of f‐L‐T 3 in the serum, showing their ability to be used in the diagnosis of thyroid disease (i.e., subclinical hypo‐ and hyperthyroidism). Compared with the most frequently used techniques such as immunometric assays, the limit of determination for f‐L‐T 4 obtained using the microsensor based on TEX/DP is lower than the one reported using the ELISA kit (5.15 x 10 ‐12 molL ‐1 ), RIA kit (2.57 x 10 ‐12 molL ‐1 ) and CLEIA (1.59 x 10 ‐12 molL ‐1 ) . Compared with the best results in the literature (1.29 x 10 ‐9 molL ‐1 ) for the enantiorecognition of f‐D‐T 4 using the TEX/DP‐based microsensor, it registered a lower limit of determination and also using the IQ/DP‐based microsensor for the detection of to f‐L‐T 3 was obtained with a very close limit of determination to the ELISA kit.…”

Section: Resultsmentioning

confidence: 62%

“…Compared with the most frequently used techniques such as immunometric assays, the limit of determination for f-L-T 4 obtained using the microsensor based on TEX/DP is lower than the one reported using the ELISA kit (5.15 x 10 -12 molL -1 ), RIA kit (2.57 x 10 -12 molL -1 ) and CLEIA (1.59 x 10 -12 molL -1 ). 26 Compared with the best results in the literature (1.29 x 10 -9 molL -1 ) 27 for the enantiorecognition of f-D-T 4 using the TEX/DP-based microsensor, it registered a lower limit of determination and also using the IQ/DP-based microsensor for the detection of to f-L-T 3 was obtained with a very close limit of determination to the ELISA kit.…”

Section: Results and Discussion Response Characteristics Of The Stochmentioning

confidence: 67%

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Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T₃, L‐T₄, and D‐T₄

et al. 2015

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A fast screening method of whole blood was proposed for enantiorecognition of free L-T3 , L-T4 , and D-T4 . Stochastic microsensors based on four inulins (IN, IQ, TEX, and HD) immobilized on diamond paste (DP) were used for recognition of free L-T3 , L-T4 , and D-T4 . For the enantiorecognition of free L-T4 and D-T4 in whole blood and pharmaceutical samples, the best microsensor was the one based on TEX/DP (wide linear concentration ranges, and low limits of quantification). The best limit of detection for the assay of free L-T3 (400 fmol/L) was recorded using the microsensors based on HD/DP, while for the assay of free L-T4, and D-T4 the best limit of determination (1 pmol/L) was recorded using the TX/DP-based microsensor. For the enantiorecognition of free L-T3 in whole blood and pharmaceutical samples the best microsensor was the one based on HD/DP (the wider linear concentration range, and the lower limit of quantification - of pmol/L magnitude order). For the enantiorecognition of free L-T3 in whole blood and pharmaceutical samples the best microsensor was the one based on HD/DP (the wider linear concentration range, and the lower limit of quantification - of pmol/L magnitude order). Free L-T3 , L-T4 , and D-T4 were recovered with high reliabilities in whole blood samples (recoveries higher than 99.00%, with RSD values lower than 1.00%) and pharmaceutical samples (recoveries higher than 95.00% with RSD values lower than 1.00%).

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Section: Resultsmentioning

confidence: 62%

Section: Results and Discussion Response Characteristics Of The Stochmentioning

confidence: 67%

Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T₃, L‐T₄, and D‐T₄

et al. 2015

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“…Since the Ag+Ag*+Ab incubation is carried out in a homogenous solution in this assay, the binding equilibrium can be reached within 15 min. However, an incubation time of 45 min or longer is normally required in the widely used heterogeneous immunoassays based on Ab-coated micro-plates [2] or magnetic particles [32] due to the limited interaction among the analyte, the reagents and the immobilized antibody. Further, the MCE separation of Ag*-Ab from Ag* in this assay is very quick (< 1 min), which represents a distinct advantage of MCE over other separation techniques such as HPLC and capillary electrophoresis (CE).…”

Section: Resultsmentioning

confidence: 99%

Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis

Huang

Zhao

Shi

et al. 2010

Analytical Biochemistry

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A homogenous chemiluminescent immunoassay of thyroxine (T4) enhanced by microchip electrophoresis separation has been developed. The method deployed the competitive immunoreaction of T4 and horseradish peroxidase (HRP)-labeled T4 (HRP-T4) with anti-T4 mouse monoclonal antibody (Ab). HRP-T4 and the HRP-T4-Ab complex were separated and quantified by using microchip electrophoresis (MCE) with chemiluminescence (CL) detection. Highly sensitive CL detection was achieved by means of HPR-catalyzed luminol-H 2 O 2 reaction. Due to the effective MCE separation, the CL analytical signal was less prone to sample matrix interference. Under the selected assay conditions, the MCE separation was accomplished within 60 sec. The linear range for T4 were 5-250 nM with a detection limit of 2.2 nM (S/N = 3). The present method was successfully applied for the quantification of T4 in human serum samples. It was demonstrated that the present MCE-CL enhanced competitive immunoassay was quick, sensitive, and highly selective. It may serve as a tool for clinical analysis of T4 to assist diagnosis of thyroid gland functions.

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“…The conjugates of SAV and MSPs are widely utilized for bioaffinity separation, detection and indirect immobilization of biotinylated biomolecules, 19,20,[33][34][35]38 and are pivotal biomaterials in reagent kits for automated chemiluminescence immunoassays in clinical laboratories. [41][42][43] Hence, we examined the immobilization of SAV on MSP-PEG-COOH via N-acylation. MSP-PEG-D4 after the activation of carboxyl groups immobilized SAV at about 10 mg/g MSPs via N-acylation ( Figure 9).…”

Section: Immobilization Of Streptavidinmentioning

confidence: 99%

Facile one-step coating approach to magnetic submicron particles with poly(ethylene glycol) coats and abundant accessible carboxyl groups

Long¹,

Yang²,

Zhang³

et al. 2013

IJN

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PurposeMagnetic submicron particles (MSPs) are pivotal biomaterials for magnetic separations in bioanalyses, but their preparation remains a technical challenge. In this report, a facile one-step coating approach to MSPs suitable for magnetic separations was investigated.MethodsPolyethylene glycol) (PEG) was derived into PEG-bis-(maleic monoester) and maleic monoester-PEG-succinic monoester as the monomers. Magnetofluids were prepared via chemical co-precipitation and dispersion with the monomers. MSPs were prepared via one-step coating of magnetofluids in a water-in-oil microemulsion system of aerosol-OT and heptane by radical co-polymerization of such monomers.ResultsThe resulting MSPs contained abundant carboxyl groups, exhibited negligible nonspecific adsorption of common substances and excellent suspension stability, appeared as irregular particles by electronic microscopy, and had submicron sizes of broad distribution by laser scattering. Saturation magnetizations and average particle sizes were affected mainly by the quantities of monomers used for coating magnetofluids, and steric hindrance around carboxyl groups was alleviated by the use of longer monomers of one polymerizable bond for coating. After optimizations, MSPs bearing saturation magnetizations over 46 emu/g, average sizes of 0.32 μm, and titrated carboxyl groups of about 0.21 mmol/g were obtained. After the activation of carboxyl groups on MSPs into N-hydroxysuccinimide ester, biotin was immobilized on MSPs and the resulting biotin-functionalized MSPs isolated the conjugate of streptavidin and alkaline phosphatase at about 2.1 mg/g MSPs; streptavidin was immobilized at about 10 mg/g MSPs and retained 81% ± 18% (n = 5) of the specific activity of the free form.ConclusionThe facile approach effectively prepares MSPs for magnetic separations.

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Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum

Cited by 37 publications

References 32 publications

Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T₃, L‐T₄, and D‐T₄

Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T₃, L‐T₄, and D‐T₄

Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis

Facile one-step coating approach to magnetic submicron particles with poly(ethylene glycol) coats and abundant accessible carboxyl groups

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Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum

Cited by 37 publications

References 32 publications

Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T3, L‐T4, and D‐T4

Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T3, L‐T4, and D‐T4

Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis

Facile one-step coating approach to magnetic submicron particles with poly(ethylene glycol) coats and abundant accessible carboxyl groups

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Product

Resources

About

Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T₃, L‐T₄, and D‐T₄

Fast Screening of Whole Blood Samples and Pharmaceutical Compounds for Enantiorecognition of Free L‐T₃, L‐T₄, and D‐T₄