Magnetic, optical gold nanorods for recyclable photothermal ablation of bacteria

Ramasamy, Mohankandhasamy; Lee, Su Seong; Yi, Dong Kee; Kim, Kwangmeyung

doi:10.1039/c3tb21310b

Cited by 58 publications

(43 citation statements)

References 40 publications

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“…Phototherapy is widely used, and combines pulsed laser and nanoparticles such as AuNPs that are able to absorb light radiation and convert this into heat energy, which in turn has an antibacterial effect. [27][28][29] In our study, the maximum antibacterial effect was found for nanoparticles exposed to irradiation. If we modify the surface of metal nanoparticles, we can control and target the desired antibacterial effect or other target drug delivery.…”

supporting

confidence: 53%

Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles

Govindaraju

Ramasamy

Baskaran

et al. 2015

IJN

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Here we report a novel method for the synthesis of glucosamine-functionalized gold nanoparticles (GlcN-AuNPs) using biocompatible and biodegradable glucosamine for antibacterial activity. GlcN-AuNPs were prepared using different concentrations of glucosamine. The synthesized AuNPs were characterized for surface plasmon resonance, surface morphology, fluorescence spectroscopy, and antibacterial activity. The minimum inhibitory concentrations (MICs) of the AuNPs, GlcN-AuNPs, and GlcN-AuNPs when irradiated by ultraviolet light and laser were investigated and compared with the MIC of standard kanamycin using Escherichia coli by the microdilution method. Laser-irradiated GlcN-AuNPs exhibited significant bactericidal activity against E. coli. Flow cytometry and fluorescence microscopic analysis supported the cell death mechanism in the presence of GlcN-AuNP-treated bacteria. Further, morphological changes in E. coli after laser treatment were investigated using atomic force microscopy and transmission electron microscopy. The overall results of this study suggest that the prepared nanoparticles have potential as a potent antibacterial agent for the treatment of a wide range of disease-causing bacteria.

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supporting

confidence: 53%

Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles

Govindaraju

Ramasamy

Baskaran

et al. 2015

IJN

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“…Evaluation of the temperature was done every 30 seconds with a digital thermometer (FLUKE SI II) and the subsequent observation of the nanorods using the TEM [14]. 4 CFU⋅mL −1 in each well) [15].…”

Section: Characterization Of Photothermal Efficiency Of Aunrsmentioning

confidence: 99%

Antibacterial and Antibiofilm Activities of the Photothermal Therapy Using Gold Nanorods against Seven Different Bacterial Strains

Castillo-Martínez

Martínez-Castañón

Martínez-Gutiérrez

et al. 2015

Journal of Nanomaterials

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The objective of this work was to determine the bactericidal and antibiofilm activities of gold nanorods (AuNRs) using plasmonic photothermal therapy (PPTT) against oral microorganisms. AuNRs were synthesized by the seed and growth solution method and the gold nanoclusters were characterized with a size of 33.2 nm ± 2.23 length and 7.33 nm ± 1.60 width. The efficacy of PPTT related to its temperature was done reaching 67 ∘ C. Minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) of AuNRs and AuNRs PPTT were determined against Enterococcus faecalis, Staphylococcus aureus, Streptococcus mutans, Streptococcus sobrinus, Streptococcus oralis, Streptococcus salivarius, and Escherichia coli growth. The antibiofilm activity of AuNRs was explored by fluorescence microscopy. After experimental analyses, AuNRs PPTT shows better results in MICs and MBCs, when it was compared with AuNRs alone. The laser employed to activate the AuNRs had no antibacterial effect against oral microbes. The MICs and MBCs values were higher for S. aureus and E. coli and lower against S. oralis. Surprisingly, the AuNRs alone presented a high antibiofilm activity, inhibiting the biofilm formation of S. mutans. Altogether, these results strongly suggest that AuNRs could be an interesting option to control oral biofilms.

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“…In the 12-hour mediation process, the amine and mercapto functional groups were attached onto the Au surfaces. 32 FE-SEM analysis clearly depicts the existence of amorphous silica layer around AuNRs to form Si-AuNRs. The average particle size distributions of AuNR were measured by averaging 100 particles of each of three batches from the FE-SEM images using ImageJ software (developed by the National Institutes of Health, Bethesda, MD, USA).…”

Section: Fabrication and Characterization Of Aunrsmentioning

confidence: 99%

Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods

Ramasamy¹,

Yi²,

An³

2015

IJN

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Escherichia coli O157:H7 (O157) is a Gram negative and highly virulent bacteria found in food and water sources, and is a leading cause of chronic diseases worldwide. Diagnosis and prevention from the infection require simple and rapid analysis methods for the detection of pathogens, including O157. Endogenous membrane peroxidase, an enzyme present on the surface of O157, was used for the colorimetric detection of bacteria by catalytic oxidation of the peroxidase substrate. In this study, we have analyzed the impact of the synthesized bare gold nanorods (AuNRs) and silica-coated AuNRs on the growth of E. coli O157. Along with the membrane peroxidase activity of O157, other bacteria strains were analyzed. Different concentrations of nanorods were used to analyze the growth responses, enzymatic changes, and morphological alterations of bacteria by measuring optical density, 3,3′,5,5′-tetramethylbenzidine assay, flow cytometry analysis, and microscopy studies. The results revealed that O157 showed higher and continuous membrane peroxidase activity than other bacteria. Furthermore, O157 treated with bare AuNRs showed a decreased growth rate in comparison with the bacteria with surface modified AuNRs. Interestingly, silica-coated AuNRs favored the growth of bacteria and also increased membrane peroxidase activity. This result can be particularly important for the enzymatic analysis of surface treated AuNRs in various microbiological applicants.

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Magnetic, optical gold nanorods for recyclable photothermal ablation of bacteria

Cited by 58 publications

References 40 publications

Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles

Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles

Antibacterial and Antibiofilm Activities of the Photothermal Therapy Using Gold Nanorods against Seven Different Bacterial Strains

Enhanced detection sensitivity of Escherichia coli O157:H7 using surface-modified gold nanorods

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