Magnetic nanoparticles in microfluidic and sensing: From transport to detection

Khizar, Sumera; Halima, Hamdi Ben; Ahmad, Naveed; Zine, Nadia; Errachid, Abdelhamid; Elaı̈ssari, Abdelhamid

doi:10.1002/elps.201900377

Cited by 47 publications

(25 citation statements)

References 193 publications

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“…For instance, Rao et al investigated a microfluidic chip consisting of two inlets, an outlet, a Y-shaped merging channel, an S-shaped mixing channel, and an electroporation zone to synthesize erythrocyte membrane-coated magnetic nanoparticles [ 27 ]. Generally, MNPs-based microfluidic approaches focus on developing highly accurate sensors and biosensors, using MNPs synthesized through conventional methods [ 28 , 29 , 30 , 31 ]. Therefore, the novelty of our work is demonstrated by the currently limited available literature employing microfluidic approaches for MNPs synthesis.…”

Section: Resultsmentioning

confidence: 99%

Synthesis of Magnetite Nanoparticles through a Lab-On-Chip Device

et al. 2021

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Magnetite nanoparticles (MNPs) represent one of the most intensively studied types of iron oxide nanoparticles in various fields, including biomedicine, pharmaceutics, bioengineering, and industry. Since their properties in terms of size, shape, and surface charge significantly affects their efficiency towards the envisaged application, it is fundamentally important to develop a new synthesis route that allows for the control and modulation of the nanoparticle features. In this context, the aim of the present study was to develop a new method for the synthesis of MNPs. Specifically, a microfluidic lab-on-chip (LoC) device was used to obtain MNPs with controlled properties. The study investigated the influence of iron precursor solution concentration and flowed onto the final properties of the nanomaterials. The synthesized MNPs were characterized in terms of size, morphology, structure, composition, and stability. Results proved the formation of magnetite as a single mineral phase. Moreover, the uniform spherical shape and narrow size distribution were demonstrated. Optimal characteristics regarding MNPs crystallinity, uniformity, and thermal stability were obtained at higher concentrations and lower flows. In this manner, the potential of the LoC device is a promising tool for the synthesis of nanomaterials by ensuring the necessary uniformity for all final applications.

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Section: Resultsmentioning

confidence: 99%

Synthesis of Magnetite Nanoparticles through a Lab-On-Chip Device

et al. 2021

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“…(2020) . In addition, lab-on-a-chip and microfluidics can also be used to develop portable, sensitive, and cost-effective biosensing systems for Cas12a-mediated detection ( Khizar et al., 2020 ; Ramachandran et al., 2020 ). In such systems, the RAA and CRISPR reagents can be integrated onto a chip in advance, which enables the application of CRISPR-Cas-mediated steps in various settings, namely, clinics, mobile testing stations, and rural areas.…”

Section: Discussionmentioning

confidence: 99%

Visual Identification and Serotyping of Toxigenic Vibrio cholerae Serogroups O1 and O139 With CARID

Lü

Chen

et al. 2022

Front. Cell. Infect. Microbiol.

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There is a growing demand for rapid, sensitive, field-deployable nucleic acid tests for cholera, which usually occurs in rural areas. In this study, we developed a Cas12a-assisted rapid isothermal detection (CARID) system for the detection of toxigenic V. cholerae serogroups O1 and O139 by combining recombinase-aided amplification and CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins). The results can be determined by fluorescence signal and visualized by lateral flow dipstick. We identified 154 V. cholerae strains and 129 strains of other intestinal diarrheagenic bacteria with a 100% coincidence rate. The limit of detection of CARID was 20 copies/reaction of V. cholerae genomic DNA, which is comparable to that of polymerase chain reaction (PCR) and qPCR. Multiple-CARID was also established for efficiency and economic considerations with an acceptable decrease in sensitivity. Simulated sample tests showed that CARID is suitable for complex samples. In conclusion, CARID is a rapid, sensitive, economically efficient, and portable method for the detection of V. cholerae, which makes it suitable for field responses to cholera.

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“…In the scenario where magnetic beads are functionalized with specific reagents, they enable target detection by capturing the analyte in the fluid sample (Wu and Voldman 2020). In addition, magnetic beads can be controlled with an external array of electromagnets in such a manner that they can also transport analytes within various chambers inside a microfluidic chip for further analyte processing (Khizar et al 2020). In this way, they offer a double functionality.…”

Section: Introductionmentioning

confidence: 99%

Magnetic bead mixing in a microfluidic chamber induced by an in-plane rotating magnetic field

Shanko

Buul

Wang

et al. 2022

Microfluid Nanofluid

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Magnetic microbeads have been widely used for the capturing of biomarkers, as well as for microfluidic mixing for point-of-care diagnostics. In magnetic micromixing, microbead motion is generated by external electromagnets, inducing fluid kinetics, and consequently mixing. Here, we utilize an in-plane rotating magnetic field to induce magnetic bead mixing in a circular microfluidic chamber that allows better access with (optical) readout than for existing micromixing approaches. We analyze the magnetic bead dynamics, the induced fluid profiles and we quantify the mixing performance of the system. The rotating field causes the combination of (1) a global rotating flow counter to the external field rotation induced by magnetic particles moving along the chamber side wall, with (2) local flow perturbations induced by rotating magnetic bead clusters in the central area of the chamber, rotating in the same direction as the external field. This combination leads to efficient mixing performance within 2 min of actuated magnetic field. We integrate magnetic mushroom-shaped features around the circumference of the chamber to generate significantly higher global fluid velocities compared with the no-mushroom configuration, but this results in less efficient mixing due to the absence of the central rotating bead clusters. To validate and understand the experimental results and to predict further enhancement of mixing, we carry out numerical simulations of induced fluid profiles and their corresponding mixing indices, and we explore the additional effect of integrating geometrical structures. The micromixing method we introduce here is particularly suitable for microfluidic devices in which the biochemical assay happens in a microfluidic chamber under no-flow conditions, i.e., with initially stagnant fluids, and for which the time-to-result is critical, such as in point-of-care diagnostics.

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Magnetic nanoparticles in microfluidic and sensing: From transport to detection

Cited by 47 publications

References 193 publications

Synthesis of Magnetite Nanoparticles through a Lab-On-Chip Device

Synthesis of Magnetite Nanoparticles through a Lab-On-Chip Device

Visual Identification and Serotyping of Toxigenic Vibrio cholerae Serogroups O1 and O139 With CARID

Magnetic bead mixing in a microfluidic chamber induced by an in-plane rotating magnetic field

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