Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

Yang, Shieh‐Yueh; Chieh, Jen Jie; Wang, W. C.; Yu, Chien-Lin; Hing, N. S.; Horng, Herng-Er; Hong, Chin Yih; Yang, Hong-Chang; Chang, Chiung-Fen; Lin, Hung Yin

doi:10.1016/j.jmmm.2010.10.011

Cited by 38 publications

(33 citation statements)

References 16 publications

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“…Thus, the frequency of the peak of the low-frequency magnetic susceptibility corresponds to the resonant frequency of the oscillation of the magnetic dipole moment inside domains. The χ ac peak indicates the spin exchange-coupling interaction and dipole moment of domain under frequency [27]. It is reasonably concluded that the physical meaning peaks of the low frequency susceptibility indicate the magnetic exchange coupling between two CoFeB layers.…”

Section: Resultsmentioning

confidence: 99%

“…These values are larger than compared for Fe 40 Pd 40 B 20 ( X Å)/ZnO(500 Å) and suitable for low-frequency magnetic media applications [26]. The magnetic material under the external AC magnetic field shows a magnetic property called multiple-frequency AC magnetic susceptibility χ ac [27]. The origin of χ ac is due to the association between magnetic spin interactions [27].…”

Section: Introductionmentioning

confidence: 99%

“…The magnetic material under the external AC magnetic field shows a magnetic property called multiple-frequency AC magnetic susceptibility χ ac [27]. The origin of χ ac is due to the association between magnetic spin interactions [27]. The frequency of the applied AC magnetic field equals the frequency of oscillation of the magnetic dipole.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Low-Frequency AC Magnetic Susceptibility and Magnetic Properties of CoFeB/MgO/CoFeB Magnetic Tunnel Junctions

2014

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In this investigation, the low-frequency alternate-current (AC) magnetic susceptibility (χac) and hysteresis loop of various MgO thickness in CoFeB/MgO/CoFeB magnetic tunneling junction (MTJ) determined coercivity (Hc) and magnetization (Ms) and correlated that with χac maxima. The multilayer films were sputtered onto glass substrates and the thickness of intermediate barrier MgO layer was varied from 6 to 15 Å. An experiment was also performed to examine the variation of the highest χac and maximum phase angle (θmax) at the optimal resonance frequency (fres), at which the spin sensitivity is maximal. The results reveal that χac falls as the frequency increases due to the relationship between magnetization and thickness of the barrier layer. The maximum χac is at 10 Hz that is related to the maximal spin sensitivity and that this corresponds to a MgO layer of 11 Å. This result also suggests that the spin sensitivity is related to both highest χac and maximum phase angle. The corresponding maximum of χac is related to high exchange coupling. High coercivity and saturation magnetization contribute to high exchange-coupling χac strength.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Low-Frequency AC Magnetic Susceptibility and Magnetic Properties of CoFeB/MgO/CoFeB Magnetic Tunnel Junctions

2014

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“…Several papers have demonstrated that IMR can be applied to assay proteins, 8 viruses, 9 chemicals, and nucleic acids 10 once suitable bioprobes are immobilized onto the magnetic nanoparticles. However, investigations of the detection threshold and interference tests of IMR have been very rare.…”

Section: Introductionmentioning

confidence: 99%

Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles

Huang¹,

Yang

Hong³

et al. 2012

IJN

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Some previous reports have already shown the characterizations of immunomagnetic reduction (IMR). The assay technology involves the utilities of biofunctionalized magnetic nanoparticles to label target biomolecules. However, the detection threshold and interference tests for IMR have not been investigated in detail. In this study, alpha-fetoprotein (AFP) was used as a target biomolecule. The signals for AFP solutions of various concentrations, or with interfering materials, were detected via IMR. These samples were also used for characterizing the detection threshold and interference with enzyme-linked immunosorbent assay (ELISA). The results of assaying AFP level with IMR and ELISA were compared. The detection threshold for assaying AFP with IMR was found to be 3 ng/mL, which is 15 times lower than that of ELISA, and definitely suppresses false negative. For the interfering materials noted commonly in serum such as hemoglobin, bilirubin, triglyceride, and vascular endothelial growth factor, there was no detectable interfering effect when assaying AFP with IMR. Several serum samples from normal people and liver-tumor-bearing patients were used for the detections of AFP concentration via IMR. These results reveal the feasibilities of assaying AFP in blood using IMR, as well as achieving high-sensitive and high-specific assay for AFP.

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“…Therefore, a larger reduction in of reagents, i.e., , can be expected. According to the reported papers, the feasibilities of analyzing proteins [11], viruses [12], nuclei acids [13] using IMR have been demonstrated. These results reveal several unique merits for IMR.…”

mentioning

confidence: 99%

Experimental Study on Low-Detection Limit for Immunomagnetic Reduction Assays by Manipulating Reagent Entities

Yang¹,

Yang

Horng

et al. 2013

IEEE Trans.on Nanobioscience

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The low limit of detection (LLD) plays an important role in biomolecular assays, especially for early-stage assays. Biomolecular detections usually involve the use of two main elements: a reagent and an analyzer, which both greatly contribute to the LLD. In this work, the relationships among the LLD and reagent-related factors are investigated. The to-be-detected biomolecule is c-reactive protein (CRP) as an example. The assay method is immunomagnetic reduction (IMR). The components of reagent are Fe(3)O(4) magnetic nanoparticles bio-functionalized with antibodies against CRP, dispersed in pH-7.4 phosphate buffered saline solution. Several key factors of the reagent, such as particle concentration, volume ratio of reagent to sample, and particle size, are manipulated to optimize the LLD of detecting CRP.

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Magnetic nanoparticles for high-sensitivity detection on nucleic acids via superconducting-quantum-interference-device-based immunomagnetic reduction assay

Cited by 38 publications

References 16 publications

Effect of Low-Frequency AC Magnetic Susceptibility and Magnetic Properties of CoFeB/MgO/CoFeB Magnetic Tunnel Junctions

Effect of Low-Frequency AC Magnetic Susceptibility and Magnetic Properties of CoFeB/MgO/CoFeB Magnetic Tunnel Junctions

Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles

Experimental Study on Low-Detection Limit for Immunomagnetic Reduction Assays by Manipulating Reagent Entities

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