Magnetic nanoparticles for efficient cell transduction with Semliki Forest virus

Ķūrēna, Baiba; Vežāne, Aleksandra; Skrastiņa, Dace; Trofimova, Olga; Zajakina, Anna

doi:10.1016/j.jviromet.2017.03.008

Cited by 7 publications

(8 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Positively-charged NPs displayed rapid and effective isolation and concentration of VPs, resulting in higher TE than negatively charged NPs. 500 In general, VPs can be coated with modified SPIONs through simple incubation due to electrostatic interactions to obtain hybrid particles. 487,492 Beside this, covalent attachment of VPs onto SPIONs is regularly achieved by EDC-NHS chemistry coupling carboxy-functionalized SPIONs to the lysine residues on viral capsid.…”

Section: Reviewmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

View full text Add to dashboard Cite

show abstract

Section: Reviewmentioning

confidence: 99%

Materials promoting viral gene delivery

Kaygisiz

Synatschke

2020

Biomater. Sci.

View full text Add to dashboard Cite

show abstract

“…The recombinant Semliki forest virus (SFV) pSFVenh/Luc, encoding firefly luciferase gene, was produced as previously described [17,18]. The virus-containing cell medium was harvested and concentrated by ultracentrifugation through two sucrose cushions, as previously described [19].…”

Section: Virus Preparation and Membrane Filtration Proceduresmentioning

confidence: 99%

“…The tubes were then centrifuged for 20 s at 500× g (3500 rpm) by FVL-2400N Combi-Spin, Mini-Centrifuge/Vortex (Biosan, Riga, Latvia) to allow the liquid to pass (filtrate) through the material under low pressure conditions. The filtrated virus samples were collected (at least 20 µL each) and used for BHK-21 cell infection in 24-well plate as previously described [18]. Briefly, 10 µL of the virus sample in duplicate was mixed with 190 µL PBS (containing M 2+ and Ca 2+ ) and incubated with BHK-21 cells for 1 h at 37 • C, 5% CO 2 , then 800 µL of BHK medium (1% fetal bovine serum) was added.…”

Section: Virus Preparation and Membrane Filtration Proceduresmentioning

confidence: 99%

Characterization of Novel Biopolymer Blend Mycocel from Plant Cellulose and Fungal Fibers

et al. 2021

Self Cite

View full text Add to dashboard Cite

In this study unique blended biopolymer mycocel from naturally derived biomass was developed. Softwood Kraft (KF) or hemp (HF) cellulose fibers were mixed with fungal fibers (FF) in different ratios and the obtained materials were characterized regarding microstructure, air permeability, mechanical properties, and virus filtration efficiency. The fibers from screened Basidiomycota fungi Ganoderma applanatum (Ga), Fomes fomentarius (Ff), Agaricus bisporus (Ab), and Trametes versicolor (Tv) were applicable for blending with cellulose fibers. Fungi with trimitic hyphal system (Ga, Ff) in combinations with KF formed a microporous membrane with increased air permeability (>8820 mL/min) and limited mechanical strength (tensile index 9–14 Nm/g). HF combination with trimitic fungal hyphae formed a dense fibrillary net with low air permeability (77–115 mL/min) and higher strength 31–36 Nm/g. The hyphal bundles of monomitic fibers of Tv mycelium and Ab stipes made a tight structure with KF with increased strength (26–43 Nm/g) and limited air permeability (14–1630 mL/min). The blends KF FF (Ga) and KF FF (Tv) revealed relatively high virus filtration capacity: the log10 virus titer reduction values (LRV) corresponded to 4.54 LRV and 2.12 LRV, respectively. Mycocel biopolymers are biodegradable and have potential to be used in water microfiltration, food packaging, and virus filtration membranes.

show abstract

“…The pSFV1 and pSFV-Helper1 plasmids [40] were generously provided by H. Garoff (Karolinska Institute, Stockholm, Sweden). The pSFV/IFNg and SFV/DS-Red vectors were generated in our lab as described previously [35,41].…”

Section: Expression Vectorsmentioning

confidence: 99%

Alphavirus-driven Interferon Gamma (IFNg) Expression Inhibits Tumour Growth in Orthotopic 4T1 Breast Cancer Model

Trofimova¹,

Korotkaja²,

Skrastiņa³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Interferon gamma (IFNg) is a pleiotropic cytokine that can potentially reprogramme the tumour microenvironment. However, the antitumour immunomodulatory properties of IFNg still need to be validated due to variable therapeutic outcomes in preclinical and clinical studies. We developed a replication-deficient Semliki Forest virus vector expressing IFNg (SFV/IFNg) and evaluated its immunomodulatory antitumour potential in vitro in a model of 3D spheroids and in vivo in immunocompetent 4T1 mouse breast cancer model. We demonstrated that SFV-derived IFN-g stimulated bone marrow macrophages to acquire the tumoricidal M1 phenotype in 3D nonattached conditions. Coculturing SFV/IFNg-infected 4T1 spheroids with BMDMs inhibited spheroid growth. In the orthotopic 4T1 mouse model, intratumoural administration of SFV/IFNg virus particles alone or in combination with the Pam3CSK4 TLR2/1 ligand led to significant inhibition of tumour growth compared to the administration of the control SFV/Luc virus particles. Analysis of the composition of intra-tumoural lymphoid cells isolated from tumours after SFV/IFNg treatment revealed an increase in CD4+ and CD8+ and a decrease in T-reg (CD4+/CD25+/FoxP3+) cell populations. Furthermore, a significant decrease in the populations of cells bearing myeloid cell markers CD11b, CD38 and CD206 was observed. In conclusion, the SFV/IFNg vector induces a therapeutic antitumour T-cell response and inhibits myeloid cell infiltration in treated tumours.

show abstract

Magnetic nanoparticles for efficient cell transduction with Semliki Forest virus

Cited by 7 publications

References 36 publications

Materials promoting viral gene delivery

Materials promoting viral gene delivery

Characterization of Novel Biopolymer Blend Mycocel from Plant Cellulose and Fungal Fibers

Alphavirus-driven Interferon Gamma (IFNg) Expression Inhibits Tumour Growth in Orthotopic 4T1 Breast Cancer Model

Contact Info

Product

Resources

About