Magnetic nanoparticles enhance the anticancer activity of cathelicidin LL-37 peptide against colon cancer cells

Niemirowicz, Katarzyna; Prokop, Izabela; Wilczewska, Agnieszka Z.; Wnorowska, Urszula; Piktel, Ewelina; Wątek, Marzena; Savage, Paul B.; Bucki, Robert

doi:10.2147/ijn.s76104

Cited by 64 publications

(59 citation statements)

References 41 publications

(43 reference statements)

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“…Our study clearly indicates that ceragenin CSA-13, apart from its pleiotropic antimicrobial properties48495051, possesses great potential for development of a new sporicidal agent. However, additional studies are needed to determine the exact mechanism of action involved in the activity of this compound against B. subtilis spores.…”

Section: Discussionmentioning

confidence: 63%

Sporicidal activity of ceragenin CSA-13 against Bacillus subtilis

Piktel

Pogoda

Roman

et al. 2017

Sci Rep

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Spore-forming bacteria are a class of microorganisms that possess the ability to survive in extreme environmental conditions. Morphological features of spores assure their resistance to stress factors such as high temperature, radiation, disinfectants, and drying. Consequently, spore elimination in industrial and medical environments is very challenging. Ceragenins are a new class of cationic lipids characterized by a broad spectrum of bactericidal activity resulting from amphipathic nature and membrane-permeabilizing properties. To assess the impact of ceragenin CSA-13 on spores formed by Bacillus subtilis (ATCC 6051), we performed the series of experiments confirming that amphipathic and membrane-permeabilizing properties of CSA-13 are sufficient to disrupt the structure of B. subtilis spores resulting in decreased viability. Raman spectroscopy analysis provided evidence that upon CSA-13 treatment the number of CaDPA-positive spores was clearly diminished. As a consequence, a loss of impermeability of the inner membranes of spores, accompanied by a decrease in spore resistance and killing take place. In addition to their broad antimicrobial spectrum, ceragenins possess great potential for development as new sporicidal agents.

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Section: Discussionmentioning

confidence: 63%

Sporicidal activity of ceragenin CSA-13 against Bacillus subtilis

Piktel

Pogoda

Roman

et al. 2017

Sci Rep

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“…For instance, theranostics and drug delivery strategies using magnetic anti-cancer drugs are expected to bring new hope for cancer therapy [40]. Indeed, Niemirowicz et al reported that magnetic nanoparticles enhance the anticancer activity of LL-37 against colon cancer cells [41]. Moreover, using mesoporous silica nanoparticles has been investigated [42].…”

Section: Discussionmentioning

confidence: 99%

miR-663a regulates growth of colon cancer cells, after administration of antimicrobial peptides, by targeting CXCR4-p21 pathway

et al. 2017

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BackgroundAntimicrobial peptides (AMPs) play important roles in the innate immune system of all life forms and recently have been characterized as multifunctional peptides that have a variety of biological roles such as anticancer agents. However, detailed mechanism of antimicrobial peptides on cancer cells is still largely unknown.MethodsmiRNA array and real-time qPCR were performed to reveal the behavior of miRNA in colon cancer HCT116 cells during the growth suppression induced by the AMPs. Establishment of miR-663a over-expressing HCT116 cells was carried out for the evaluation of growth both in vitro and in vivo. To identify the molecular mechanisms, we used western blotting analysis.ResultsmiR-663a is upregulated by administration of the human cathelicidin AMP, LL-37, and its analogue peptide, FF/CAP18, in the colon cancer cell line HCT116. Over-expression of miR-663a caused anti-proliferative effects both in vitro and in vivo. We also provide evidence supporting the view that these effects are attributed to suppression of the expression of the chemokine receptor CXCR4, resulting in the abrogation of phosphorylation of Akt and cell cycle arrest in G2/M via p21 activation.ConclusionsThis study contributes to the understanding of the AMPs’ mediated anti-cancer mechanisms in colon cancer cells and highlights the possibility of using AMPs and miRNAs towards developing future strategies for cancer therapy.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-016-3003-9) contains supplementary material, which is available to authorized users.

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“…For eons, antimicrobial peptides (AMPs) have played a central role in innate immunity against bacteria, fungi and lipid enveloped viruses [5][6][7][8][9]. The diversity of their primary sequences suggests independent evolution, and despite their ubiquity, bacteria generally remain susceptible to AMPs.…”

Section: Introductionmentioning

confidence: 99%

Ceragenins as Mimics of Endogenous Antimicrobial Peptides

Hashemi¹,

Holden²,

DurnaÅ³

et al. 2017

J Antimicrob Agents

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Ceragenins are small molecule mimics of endogenous antimicrobial peptides (AMPs), and as such display broadspectrum antimicrobial activity. These molecules are derived from a common bile acid and can be prepared at a large scale. Because ceragenins are not peptide based, they are not substrates for proteases. Gram-negative and positive bacteria are susceptible to ceragenins, including drug resistant organisms. Although ceragenins and colistin have common features, ceragenins retain full antibacterial activity against colistin-resistant Gram-negative bacteria. Bactericidal activity of ceragenins involves selective association with bacterial membranes followed by membrane depolarization. Due to this mechanism of action, which provides bactericidal activity against sessile bacteria, ceragenins eradicate established biofilms. Lipid-enveloped viruses (e.g. vaccinia) are deactivated by ceragenins, and topical application of a lead ceragenin decreases transmission of the virus in skin in a murine model. More recently, the activities of ceragenins against fungal pathogens have been reported, with minimum inhibition concentrations comparable to clinically used anti-fungal agents. In addition to antimicrobial activities, ceragenins have been shown to display some of the "secondary" activities attributed to AMPs. In vivo use of ceragenins to eradicate biofilms, prevent infection and accelerate bone growth demonstrate some of the types of applications in which ceragenins may be used to augment or replace activities of endogenous AMPs.

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Magnetic nanoparticles enhance the anticancer activity of cathelicidin LL-37 peptide against colon cancer cells

Cited by 64 publications

References 41 publications

Sporicidal activity of ceragenin CSA-13 against Bacillus subtilis

Sporicidal activity of ceragenin CSA-13 against Bacillus subtilis

miR-663a regulates growth of colon cancer cells, after administration of antimicrobial peptides, by targeting CXCR4-p21 pathway

Ceragenins as Mimics of Endogenous Antimicrobial Peptides

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