Magnetic nanoparticle DNA labeling for individual bacterial cell detection and recovery

Pivetal, Jérémy; Ciuta, Georgeta; Frénéa‐Robin, Marie; Haddour, Naoufel; Dempsey, Nora; Dumas-Bouchiat, Frédéric; Simonet, Pascal

doi:10.1016/j.mimet.2014.09.006

Cited by 12 publications

(10 citation statements)

References 41 publications

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“…As shown in the video (Supplementary Video), cells can be trapped under continuous flow inside the microfluidic device, meaning that labeled cells can be separated from unlabeled ones: When the sample is injected in the device, only the target (magnetically functionalized) cells are trapped, while the rest of the mixture moves toward the device outlet. Then, as previously studied by our team (Osman et al, 2013;Pivetal et al, 2014a), the trapped cells can be recovered by simply increasing the flow rate. These results demonstrate the feasibility of the HCR-MISH method to capture eukaryotic cells such as yeast.…”

Section: Hybridization Chain Reaction-magnetic In Situ Hybridization On Eukaryotic Cellsmentioning

confidence: 95%

Eukaryotic Cell Capture by Amplified Magnetic in situ Hybridization Using Yeast as a Model

et al. 2021

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A non-destructive approach based on magnetic in situ hybridization (MISH) and hybridization chain reaction (HCR) for the specific capture of eukaryotic cells has been developed. As a prerequisite, a HCR-MISH procedure initially used for tracking bacterial cells was here adapted for the first time to target eukaryotic cells using a universal eukaryotic probe, Euk-516R. Following labeling with superparamagnetic nanoparticles, cells from the model eukaryotic microorganism Saccharomyces cerevisiae were hybridized and isolated on a micro-magnet array. In addition, the eukaryotic cells were successfully targeted in an artificial mixture comprising bacterial cells, thus providing evidence that HCR-MISH is a promising technology to use for specific microeukaryote capture in complex microbial communities allowing their further morphological characterization. This new study opens great opportunities in ecological sciences, thus allowing the detection of specific cells in more complex cellular mixtures in the near future.

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Section: Hybridization Chain Reaction-magnetic In Situ Hybridization On Eukaryotic Cellsmentioning

confidence: 95%

Eukaryotic Cell Capture by Amplified Magnetic in situ Hybridization Using Yeast as a Model

et al. 2021

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“…Polymeric nanocarriers, such as polyethylenimine (PEI), poly (L-lysine) (PLL), poly [2-(dimethylamino) ethyl methacrylate] (PDMAEMA), polyamidoamine (PAMAM), chitosan and poly (amino-co-ester) s (PAEs), play an important role in gene delivery. Polymeric nanocarriers always combine with other materials, which include magnetic nanoparticle (Pivetal et al, 2014), grapheme oxide (Varela et al, 2014), and silver (Mishra et al, 2015), to make gene delivery more efficient.…”

Section: Nanomaterial-mediated Transformationmentioning

confidence: 99%

Genetic transformation of fungi

He¹,

Feng²,

Lu³

et al. 2017

Int. J. Dev. Biol.

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Transferring DNA into cells is an essential research method for molecular cloning and gene function studies. As molecular biology and materials physics develop, more and more new transformation methods have been applied to mammalian cells. Some techniques have been successfully developed for several types of fungi, but their efficiencies are extremely low. To better study the functional genes of fungi, and to improve the characteristics of fungi in an easy, safe and reliable way, many investigations have been conducted to effectively develop such technologies for a wide variety of species and to increase the efficiency and reproducibility of genetic transformation. The objective of this paper is to review the latest development of transformation methods used for the genetic transformation of fungi, including several promising transformation approaches, together with their advantages and drawbacks, which may open up novel methods for fungi research.

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“…In this case, MNPs have a higher magnetic strength than their oxide, which can be used as a superparamagnetic nanoparticle. Therefore, the formation of a coating on these nanoparticles could be preventing of perception and oxidation of MNPs [30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems

Naderlou

Salouti

Amini

et al. 2020

Artificial Cells, Nanomedicine, and Biotechnology

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Staphylococcus aureus is an important infectious factor in the food industry and hospital infections. Many methods are used for detecting bacteria but they are mostly time-consuming, poorly sensitive. In this study, a nano-biosensor based on iron nanoparticles (MNPs) was designed to detect S. aureus. MNPs were synthesized and conjugated to Biosensors. Then S. aureus was lysed and nano-biosensor (MNP-TiO 2 -AP-SMCC-Biosensors) was added to the lysed bacteria. After bonding the bacterial genome to the nano-biosensor, MNPs were separated by a magnet. Bacterial DNA was released from the surface of nano-biosensor and researched by Nano-drop spectrophotometry. The results of SEM and DLS revealed that the size of MNPs was 20-25 nm which increased to 38-43 nm after modification and addition of biosensors. The designed nano-biosensor was highly sensitive and specific for the detection of S. aureus. The limit of detection (LOD) was determined as 230 CFU mL À1 . There was an acceptable linear correlation between bacterial concentration and absorption at 3.7 Â 10 2 -3.7Â 10 7 whose linear diagram and regression was Y ¼ 0.242X þ 2.08 and R 2 ¼ .996. Further, in the presence of other bacteria as a negative control, it was absolutely specific. The sensitivity of the designed nano-biosensor was investigated and compared through PCR.

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Magnetic nanoparticle DNA labeling for individual bacterial cell detection and recovery

Cited by 12 publications

References 41 publications

Eukaryotic Cell Capture by Amplified Magnetic in situ Hybridization Using Yeast as a Model

Eukaryotic Cell Capture by Amplified Magnetic in situ Hybridization Using Yeast as a Model

Genetic transformation of fungi

Enhanced sensitivity and efficiency of detection of Staphylococcus aureus based on modified magnetic nanoparticles by photometric systems

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