Magnetic‐Nanoparticle‐Based Immunoassays‐on‐Chip: Materials Synthesis, Surface Functionalization, and Cancer Cell Screening

Zhu, Ying; Kekalo, Katsiaryna; Ndong, Christian; Huang, Yun; Shubitidze, Fridon; Griswold, Karl E.; Baker, I.; Zhang, John X. J.

doi:10.1002/adfm.201504176

Cited by 37 publications

(37 citation statements)

References 219 publications

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“…A series of techniques have been established to enrich or capture CTCs for cell enumeration and phenotype analysis . Immunomagnetic separation represents a promising approach for clinical use because of its capability to rapidly process a large volume of samples as well as easy operation and facile cell recovery by the end users . However, most of the magnetic bead‐based separation suffers from low capture purities (CPs) that compromise detection accuracy and complicate downstream analysis .…”

Section: Introductionmentioning

confidence: 99%

DNA‐Templated Magnetic Nanoparticle‐Quantum Dot Polymers for Ultrasensitive Capture and Detection of Circulating Tumor Cells

Wang

Shen

et al. 2018

Adv Funct Materials

101

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Circulating tumor cell (CTC) enumeration and analysis has emerged as an important platform for cancer diagnosis and prognosis. A great challenge, however, is to efficiently capture low abundant CTCs with high purity from blood samples in a rapid and high-throughput manner for accurate and sensitive CTC detection. Herein, a new class of DNA-templated magnetic nanoparticle-quantum dot (QD)-aptamer copolymers (MQAPs) is developed for rapid magnetic isolation of CTCs from human blood with high capture efficiency and purity approaching 80%. The phenotype of CTCs is simultaneously profiled with QD photoluminescence (PL) at single cell level. These MQAPs are constructed through hybridization chain reaction to achieve amplified magnetic response, extraordinary binding selectivity for target cells over background cells, and ultra bright ensemble QD PL for single cell detection. MQAPs are free from nonspecific binding that would otherwise compromise the capture purity of target cells. As a result, facile isolation and enumeration of rare CTCs in blood samples could be achieved in 20 min with high sensitivity and accuracy.

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Section: Introductionmentioning

confidence: 99%

DNA‐Templated Magnetic Nanoparticle‐Quantum Dot Polymers for Ultrasensitive Capture and Detection of Circulating Tumor Cells

Wang

Shen

et al. 2018

Adv Funct Materials

101

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“…Wet chemical methods have been studied more widely than physical ones, since they can provide a higher level of control over the size, composition, magnetic properties, and the form of magnetic nanopar-ticles. This is especially important for screening cells in a liquid medium (Zhu et al, 2016).…”

Section: Tablementioning

confidence: 99%

Modern magnetic immunoassay: Biophysical and biochemical aspects

Galkin

Besarab

Pysmenna

et al. 2017

Regul. Mech. Biosyst.

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In this review article an analysis of the biochemical and biophysical aspects of modern magnetic immunoassay (MIA) is conducted and additionally the problems and perspectives of its application in biology, biotechnology and medicine are defined. Magnetic immunoassay should be considered as an evolutionary extension of the classical immunoassay. MIA can have many variants of modifications, similar to the classic immunoenzymatic assay. The key distinctive element of the MIA is the use of magnetic particles (MPs), which are usually nanoparticles. MPs in the MIA can act as a marker for detection, or the solid phase at which the immunochemical reaction takes place. MIA possesses basic advantages over classical immunoassay methods: thanks to the unique magnetic properties of the MPs and the ability to manipulate it in the external magnetic field, it is possible to increase the informative value of the analysis (first of all, sensitivity and specificity), as well as the rigid requirements for “purity” of tested samples. For the purposes of immunoassay, magnetic particles of size from 10 to 200 nm are important, since such particles are in a superparamagnetic state, in the absence of strong magnetic fields; they are not agglomerated in a liquid medium. The size of the spherical particle determines the rate of sedimentation and mobility in the solution. The outer polymeric membrane serves as a matrix in which the surface functional groups are added, and also protects the core of the metal from the external environment. The outer shell may also consist of agarose, cellulose, porous glass, silicon dioxide etc. There are several strategies for the synthesis of nanoparticles: mechanical (dispersion), physical (gas phase deposition), wet chemical methods (chemical comprecipitation, thermal decomposition, methods of micro emulsion, hydrothermal reactions) and physico-chemical methods. Also used are magnesite nanoparticles of biogenic origin. Magnetic particles may function, and this is important for immunoassay. Surface functional groups include carboxylic, amino, epoxy, hydroxyl, tosyl, and N-hydroxysuccinate-activated groups. Magnetic spherical particles usually interact with surface molecules such as streptovidine, biotin, protein A, protein G, and immunoglobulin etc. Directions and prospects of the development of methods of magnetic immunoassay are determined, mainly, by the development of methods for detecting or influencing magnetic particles. In this case, the classical methods of detection are electrochemical methods, electrochemiluminescence, fluorescence. More modern ones include giant magnetoresistance, superconducting quantum interference devices, surface-enhanced Raman spectroscopy, biosensors based on nonlinear magnetization, magneto-PCR immunoassay. The current trend is to combine or integrate the application of various biochemical, physical, molecular and genetic, physico-chemical detection methods. In fact, all of these benefits undoubtedly open up broad prospects for the practical application of MIA in biology, biotechnology and medicine.

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“…[3][4][5][6] Since the amount of CTCs is extremely exiguous in patients' blood, [7][8][9] there is an imperative demand to capture and enrich CTCs with high efficiency accordingly. In the last few years, multitudinous systems have been reported for the separation and enrichment of CTCs such as magnetic isolation technology, [10][11][12][13] microuidic technology [14][15][16][17] and ow cytometry. 18,19 Most of the above methods relied on a single CTC marker to capture CTC.…”

Section: Introductionmentioning

confidence: 99%

Capture and selective release of multiple types of circulating tumor cells using smart DNAzyme probes

et al. 2020

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Magnetic‐Nanoparticle‐Based Immunoassays‐on‐Chip: Materials Synthesis, Surface Functionalization, and Cancer Cell Screening

Cited by 37 publications

References 219 publications

DNA‐Templated Magnetic Nanoparticle‐Quantum Dot Polymers for Ultrasensitive Capture and Detection of Circulating Tumor Cells

DNA‐Templated Magnetic Nanoparticle‐Quantum Dot Polymers for Ultrasensitive Capture and Detection of Circulating Tumor Cells

Modern magnetic immunoassay: Biophysical and biochemical aspects

Capture and selective release of multiple types of circulating tumor cells using smart DNAzyme probes

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