Magnetic nanocomposite scaffolds combined with static magnetic field in the stimulation of osteoblastic differentiation and bone formation

Yun, Hyung-Mun; Ahn, Su−Jin; Park, Kyung-Ran; Kim, Mi-Joo; Kim, Jung‐Ju; Jin, Guang‐Zhen; Kim, Hae‐Won; Kim, Eun-Cheol

doi:10.1016/j.biomaterials.2016.01.035

Cited by 210 publications

(144 citation statements)

References 37 publications

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“…However, in the present experiments, after an initial increase, the number of blood vessels seems reduced again in the mineralized matrix, probably because of the increasing formation of new mineralized tissue. To overcome this problem, the collagen-MHA material should include small areas of collagen without MHA, where vascular structures could still develop, as shown here in the collagen layer, thus improving the vascularization of the bony layer262728.…”

Section: Discussionmentioning

confidence: 99%

Bone augmentation after ectopic implantation of a cell-free collagen-hydroxyapatite scaffold in the mouse

Calabrese

Giuffrida²,

Forte³

et al. 2016

Sci Rep

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The bone grafting is the classical way to treat large bone defects. Among the available techniques, autologous bone grafting is still the most used but, however, it can cause complications such as infection and donor site morbidity. Alternative and innovative methods rely on the development of biomaterials mimicking the structure and properties of natural bone. In this study, we characterized a cell-free scaffold, which was subcutaneously implanted in mice and then analyzed both in vivo and ex vivo after 1, 2, 4, 8 and 16 weeks, respectively. Two types of biomaterials, made of either collagen alone or collagen plus magnesium-enriched hydroxyapatite have been used. The results indicate that bone augmentation and angiogenesis could spontaneously occur into the biomaterial, probably by the recruitment of host cells, and that the composition of the scaffolds is crucial. In particular, the biomaterial more closely mimicking the native bone drives the process of bone augmentation more efficiently. Gene expression analysis and immunohistochemistry demonstrate the expression of typical markers of osteogenesis by the host cells populating the scaffold. Our data suggest that this biomaterial could represent a promising tool for the reconstruction of large bone defects, without using exogenous living cells or growth factors.

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Section: Discussionmentioning

confidence: 99%

Bone augmentation after ectopic implantation of a cell-free collagen-hydroxyapatite scaffold in the mouse

Calabrese

Giuffrida²,

Forte³

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…[11][12][13][14] Nanophase ceramics have gained increasing attention because of their superior bioactivity as well as structural and compositional similarity to bone extracellular matrix.…”

Section: 10mentioning

confidence: 99%

Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering

et al. 2017

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Objectives: Treatment of critical-sized bone defects with cells and biomaterials offers an efficient alternative to traditional bone grafts. Chitosan (CS) is a natural biopolymer that acts as a scaffold in bone tissue engineering (BTE). Polyphosphate (PolyP), recently identified as an inorganic polymer, acts as a potential bone morphogenetic material, whereas pigeonite (Pg) is a novel iron-containing ceramic. In this study, we prepared and characterized scaffolds containing CS, calcium polyphosphate (CaPP) and Pg particles for bone formation in vitro and in vivo.

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“…This value was significantly lower than that reported elsewhere for nanocomposite scaffolds incorporated with MNPs (4.8 emu g −1 ) [15] but higher than the paramagnetic nanofibrous composite film incorporated with γ-Fe 2 O 3 (0.049 emu g -1 ) [10,13]. The coated substrate has exhibited excellent osteogenesis activity despite the low level of saturation magnetization.…”

Section: Discussionmentioning

confidence: 63%

“…Optical images of the water droplet were taken after 1 min, and the ascending angles were measured. The magnetic properties of the scaffolds were measured by a vibrating sample magnetometer (VSM, Lake Shore VSM-7307) in an applied magnetic field of ±20 kOe at room temperature, in terms of saturation magnetization and hysteresis loops [15]. The VSM was calibrated using a high purity nickel sphere standard supplied by the company.…”

Section: Methodsmentioning

confidence: 99%

Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe ₃ O ₄ nanoparticles for hBMSCs

Huang

et al. 2018

R. Soc. open sci.

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Titanium (Ti) is an ideal bone substitute due to its superior bio-compatibility and remarkable corrosion resistance. However, in order to improve the osteoconduction and osteoinduction capacities in clinical applications, different kinds of surface modifications are typically applied to Ti alloys. In this study, we fabricated a tightly attached polydopamine-assisted Fe3O4 nanoparticle coating on Ti with magnetic properties, aiming to improve the osteogenesis of the Ti substrates. The PDA-assisted Fe3O4 nanoparticle coatings were characterized by scanning electron microscopy, energy dispersive spectroscopy, atomic force microscopy and water contact angle measurements. The cell attachment and proliferation rate of the human bone mesenchymal stem cells (hBMSCs) on the Ti surface significantly improved with the Fe3O4/PDA coating when compared with the pure Ti without a coating. Furthermore, the results of in vitro alkaline phosphatase (ALP) activity at 7 and 14 days and alizarin red S staining at 14 days showed that the Fe3O4/PDA coating on Ti promoted the osteogenic differentiation of hBMSCs. Moreover, hBMSCs co-cultured with the Fe3O4/PDA-coated Ti for approximately 14 days also exhibited a significantly higher mRNA expression level of ALP, osteocalcin and runt-related transcription factor-2 (RUNX2). Our in vitro results revealed that the present PDA-assisted Fe3O4 nanoparticle surface coating is an innovative method for Ti surface modification and shows great potential for clinical applications.

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Magnetic nanocomposite scaffolds combined with static magnetic field in the stimulation of osteoblastic differentiation and bone formation

Cited by 210 publications

References 37 publications

Bone augmentation after ectopic implantation of a cell-free collagen-hydroxyapatite scaffold in the mouse

Bone augmentation after ectopic implantation of a cell-free collagen-hydroxyapatite scaffold in the mouse

Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering

Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe ₃ O ₄ nanoparticles for hBMSCs

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Magnetic nanocomposite scaffolds combined with static magnetic field in the stimulation of osteoblastic differentiation and bone formation

Cited by 210 publications

References 37 publications

Bone augmentation after ectopic implantation of a cell-free collagen-hydroxyapatite scaffold in the mouse

Bone augmentation after ectopic implantation of a cell-free collagen-hydroxyapatite scaffold in the mouse

Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering

Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe 3 O 4 nanoparticles for hBMSCs

Contact Info

Product

Resources

About

Enhanced in vitro biocompatibility and osteogenesis of titanium substrates immobilized with dopamine-assisted superparamagnetic Fe ₃ O ₄ nanoparticles for hBMSCs