Magnetic nanocomposite hydrogels and static magnetic field stimulate the osteoblastic and vasculogenic profile of adipose-derived cells

Filippi, Miriam; Dasen, Boris; Guerrero, Julien; Garello, Francesca; Isu, Giuseppe; Born, Gordian; Ehrbar, Martin; Martín, Iván; Scherberich, Arnaud

doi:10.1016/j.biomaterials.2019.119468

Cited by 97 publications

(61 citation statements)

References 61 publications

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“…Critical size bone defects need adequate vascularization for reconstruction (Genova et al, 2016). Therefore, Filippi et al (2019) designed cell-loading magnetic nanocomposite hydrogels by incorporation of human adipose tissue derived stromal vascular fraction (SVF) cells into polyethylene glycol (PEG)-MNPs-based PEG hydrogels, which were further examined to enhance the activity of alkaline phosphatase (ALP), to increase the expression of osteogenesis-related genes, and to improve the mineralized extracellular matrix (ECM) formation both in vitro and in vivo. The results suggested that magnetically actuated cellladen hydrogels demonstrated more mineralization and faster vascularization in comparison with MNPs or SMF alone.…”

Section: Applications In Bone Tissue Engineeringmentioning

confidence: 99%

Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering

et al. 2020

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Tissue engineering is a promising strategy for the repair and regeneration of damaged tissues or organs. Biomaterials are one of the most important components in tissue engineering. Recently, magnetic hydrogels, which are fabricated using iron oxide-based particles and different types of hydrogel matrices, are becoming more and more attractive in biomedical applications by taking advantage of their biocompatibility, controlled architectures, and smart response to magnetic field remotely. In this literature review, the aim is to summarize the current development of magnetically sensitive smart hydrogels in tissue engineering, which is of great importance but has not yet been comprehensively viewed.

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Section: Applications In Bone Tissue Engineeringmentioning

confidence: 99%

Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering

et al. 2020

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“…Testing the changes of 16 genes under different magnetic fields by RT-PCR, they found that different magnetic field strengths have different effects on different genes (Zhang et al, 2018a ). When the magnetic field is strong, it can change the movement state of the cell (Filippi et al, 2019 ). Filippi conducted an experiment on osteoblast cells with a strong magnetic field (Filippi et al, 2019 ).…”

Section: Preparation Of Magnetic Nanomaterialsmentioning

confidence: 99%

“…When the magnetic field is strong, it can change the movement state of the cell (Filippi et al, 2019 ). Filippi conducted an experiment on osteoblast cells with a strong magnetic field (Filippi et al, 2019 ). It was found that the cells under the external magnetic field are arranged in the direction of the magnetic field, showing orientation.…”

Section: Preparation Of Magnetic Nanomaterialsmentioning

confidence: 99%

“…The direction of the magnetic induction line is perpendicular to the cell. Animal experiments have shown that magnetic fields can promote the attachment, proliferation, differentiation, and expression of growth factors such as bone morphology proteins, accelerate bone fusion, and promote the formation of new bone (Wang Z. et al, 2017 ; Guerri et al, 2018 ; Filippi et al, 2019 ). The magnetic field can also promote the fusion of bone and implant and increase bone density and calcium content to accelerate the healing of bone damage.…”

Section: Preparation Of Magnetic Nanomaterialsmentioning

confidence: 99%

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Recent Advances of Magnetic Nanomaterials in Bone Tissue Repair

et al. 2020

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The magnetic field has been proven to enhance bone tissue repair by affecting cell metabolic behavior. Magnetic nanoparticles are used as biomaterials due to their unique magnetic properties and good biocompatibility. Through endocytosis, entering the cell makes it easier to affect the physiological function of the cell. Once the magnetic particles are exposed to an external magnetic field, they will be rapidly magnetized. The magnetic particles and the magnetic field work together to enhance the effectiveness of their bone tissue repair treatment. This article reviews the common synthesis methods, the mechanism, and application of magnetic nanomaterials in the field of bone tissue repair.

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“…An increase in cell surface area here indicated that cells were developing higher tractions, which was accompanied by an increase in the expression of the osteogenic fate marker Runx2. In a separate study, magnetic NPs were dispersed in a PEG hydrogel to render it magnetic (Filippi et al, 2019). When subjected to an external magnetic field, cells from the stromal vasculature fraction of human adipose tissue were activated by movement of the NPs through the gel.…”

Section: Active Manipulation Of Matrix Mechanicsmentioning

confidence: 99%

Nanoparticles as Versatile Tools for Mechanotransduction in Tissues and Organoids

Fattah

Ranga

2020

Front. Bioeng. Biotechnol.

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Organoids are 3D multicellular constructs that rely on self-organized cell differentiation, patterning and morphogenesis to recapitulate key features of the form and function of tissues and organs of interest. Dynamic changes in these systems are orchestrated by biochemical and mechanical microenvironments, which can be engineered and manipulated to probe their role in developmental and disease mechanisms. In particular, the in vitro investigation of mechanical cues has been the focus of recent research, where mechanical manipulations imparting local as well as large-scale mechanical stresses aim to mimic in vivo tissue deformations which occur through proliferation, folding, invagination, and elongation. However, current in vitro approaches largely impose homogeneous mechanical changes via a host matrix and lack the required positional and directional specificity to mimic the diversity of in vivo scenarios. Thus, while organoids exhibit limited aspects of in vivo morphogenetic events, how local forces are coordinated to enable large-scale changes in tissue architecture remains a difficult question to address using current techniques. Nanoparticles, through their efficient internalization by cells and dispersion through extracellular matrices, have the ability to provide local or global, as well as passive or active modulation of mechanical stresses on organoids and tissues. In this review, we explore how nanoparticles can be used to manipulate matrix and tissue mechanics, and highlight their potential as tools for fate regulation through mechanotransduction in multicellular model systems.

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Magnetic nanocomposite hydrogels and static magnetic field stimulate the osteoblastic and vasculogenic profile of adipose-derived cells

Cited by 97 publications

References 61 publications

Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering

Recent Advances on Magnetic Sensitive Hydrogels in Tissue Engineering

Recent Advances of Magnetic Nanomaterials in Bone Tissue Repair

Nanoparticles as Versatile Tools for Mechanotransduction in Tissues and Organoids

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