Magnetic microsphere-based methods to study the interaction of teicoplanin with peptides and bacteria

Abstract

Teicoplanin (

Magnetic solids in analytical chemistry: A review

“…These methods have served to improve the detection limits of earlier techniques and have even been proposed as rapid screening methods [92,[145][146][147][148][149][150][151]. Tumorigenic polynuclear aromatic hydrocarbons such as ergosterol, sulfonamides or salicylic acid have been isolated by magnetic solid-phase extraction.…”

In vitroselection of DNA aptamers for the development of chemiluminescence aptasensor for neuron-specific enolase (NSE) detection

“…13 Compared with antibodies, aptamers would be promising recognition elements for bioanalysis because of their high affinity and specicity with various kinds of targets, 14 low molecular weight, reproducible synthesis, 15 facile modication, and good stability. 16 Aptamers for various targets have been selected and used for designing biosensors. Karpik et al developed an aptamer-based biosensor to detect Mucin 1 protein to monitor Prostatic cancer (PCa).…”

Bactericidal Core-Shell Paramagnetic Nanoparticles Functionalized with Poly(hexamethylene biguanide)

“…aureus membrane is rich in repeating units of peptidoglycans (PG); each PG unit consists of a disaccharide ( N -acetylglucosamine and N -acetylmuramic acid), a pentapeptide chain ( l -Ala-D- iso -Gln- l -Lys- d -Ala- d -Ala), and an attached pentaglycyl bridging segment . The interactions between antibiotics and various probes and Gram-positive bacteria are frequently modeled by the probe binding to the terminal d -Ala- d -Ala dipeptide. , In the present work, we followed this trend using a d -Ala- d -Ala- d -Ala tripeptide labeled with 5-carboxyfluorescein (5-FAM(DA) 3 ) as a model component of the bacterial membrane.…”

“…Then, the magnetic particles were collected at the bottom of the tubes and the fluorescence of the supernatant was recorded as described above. The equilibrium adsorption capacity of the particles (Γ, mol/g) was found from the following expression: normalΓ = I 0 − I normalt I 0 .1em C normalp 0 C normalN normalP where I 0 and I t are the fluorescence intensities measured before and after particle equilibration with the 5-FAM(DA) 3 peptide, respectively, C p 0 (mol/L) is the initial peptide concentration, and C NP is the nanoparticle concentration in g/L.…”