Magnetic mesoporous silica/graphene oxide based molecularly imprinted polymers for fast selective separation of bovine hemoglobin

Abstract

In this work, a facile protein imprinted polymer based on magnetic rich-amine mesoporous silica/graphene oxide was synthesized using dopamine as the functional monomer and bovine hemoglobin as the template molecule. Fe 3 O 4 nanoparticles were introduced onto graphene oxide by an inverse microemulsion method for facilitating surface polymerization dopamine imprinted layer. The protein adsorption test showed that the imprinted layer based on magnetic rich-amine mesoporous silica/graphene oxide can significantly shorten the adsorption equilibrium time of the imprinted polymer toward bovine hemoglobin. Under optimized conditions, the imprinting factor and maximum adsorption capacity of the magnetic imprinted polymer toward bovine hemoglobin was 2.09 and 164.5 mg g −1 , respectively. Several experimental parameters including temperature and pH of solution, type and volume of elution solvent, washing solvent, extraction time were investigated in detail. Combined with magnetic solid phase extraction and high performance liquid chromatography, the magnetic imprinted polymers was successfully applied to rapid separate and enrich bovine hemoglobin from bovine serum with the recoveries of 84.6-102.5%.

Factors affecting the analytical performance of molecularly imprinted mesoporous silica

“…[32][33][34][35][36] There were two methods usually used to have MIP-MS for protein: epitope imprinting [34][35][36] and protein imprinting. 32,33 In epitope imprinting, the unique fragment exposed on the target protein is used as a template to detect the targeted protein. 36 Several studies using trisodium phosphate, 34 tetrasodium pyrophosphate, 35 and phenyl phosphonic acid 36 as a template to separate protein phosphorylation.…”

“…In protein imprinting, the protein is used as a template. Xiao et al 32 synthesized magnetic mesoporous silica/graphene oxide molecularly imprinted to separate bovine hemoglobin from bovine serum. In this synthesis, bovine hemoglobin was used as a template.…”

“…In this synthesis, bovine hemoglobin was used as a template. This sorbent can be applied to separate the bovine hemoglobin from bovine serum result in high‐adsorption capacity (164.47 mg/g), selectivity and good recoveries (84.6%–102.5%) 32 …”

“…The MIP‐MS has been developed to separate different types of analytes in various samples. The analytes separated were metals, 25,30 pharmaceutical drugs, 25 natural compounds, 21 contaminations, 24,27,31 and proteins 32–36 . There were two methods usually used to have MIP‐MS for protein: epitope imprinting 34–36 and protein imprinting 32,33 .…”

“…This sorbent can be applied to separate the bovine hemoglobin from bovine serum result in high-adsorption capacity (164.47 mg/g), selectivity and good recoveries (84.6%-102.5%). 32 Materials with a mesoporous structure have a pore diameter of about 2-50 nm. 37 The mesopores are more effective than micropores (<2 nm) 37 and macropores (>50 nm) 37 because of their large surface area, 38 good thermal properties, 39 and good mechanical stability.…”