Magnetic Glyconanoparticles as a Versatile Platform for Selective Immunolabeling and Imaging of Cells

Garcı́a, Isabel; Gallo, Juan; Genicio, Nuria; Padró, Daniel; Penadés, Soledad

doi:10.1021/bc1003923

Cited by 54 publications

(49 citation statements)

References 42 publications

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“…Although different types of nanomaterials including CdS/ZnS quantum dots, [33][34][35][36][37][38] upconverting nanoparticles, 39 magnetic nanoparticles, [40][41][42][43][44][45] and carbon nanotubes, 46 have been used for the synthesis of glyconanoparticles, the intrinsic colour changes associated with the aggregation of noble metal nanoparticles have ensured that these nanoparticles are extensively used for the development of bioassays.…”

Section: Noble Metals Used To Synthesise the Nanoparticle Corementioning

confidence: 99%

Glyconanoparticles for colorimetric bioassays

Marín

Schofield

Field

et al. 2015

Analyst

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Carbohydrate molecules are involved in many of the cellular processes that are important for life. By combining the specific analyte targeting of carbohydrates with the multivalent structure and change of solution colour as a consequence of plasmonic interactions with the aggregation of metal nanoparticles, glyconanoparticles have been used extensively for the development of bioanalytical assays. The noble metals used to create the nanocore, the methodologies used to assemble the carbohydrates on the nanoparticle surface, the carbohydrate chosen for each specific target, the length of the tether that separates the carbohydrate from the nanocore and the density of carbohydrates on the surface all impact on the structural formation of metal based glyconanoparticles. This tutorial review highlights these key components, which directly impact on the selectivity and sensitivity of the developed bioassay, for the colorimetric detection of lectins, toxins and viruses.3

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Section: Noble Metals Used To Synthesise the Nanoparticle Corementioning

confidence: 99%

Glyconanoparticles for colorimetric bioassays

Marín

Schofield

Field

et al. 2015

Analyst

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“…3). The carboxyl groups available on the surface of the glycoMNPs can be used as anchoring points for further functionalization to yield (immuno)fluorescent glycoMNPs [28][29][30][31][32].…”

Section: Ligand Exchangementioning

confidence: 99%

Recent advances in biosensing using magnetic glyconanoparticles

2015

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In this critical review we discuss the most recent advances in the field of biosensing applications of magnetic glyconanoparticles. We first give an overview of the main synthetic routes to obtain magnetic-nanoparticle-carbohydrate conjugates and then we highlight their most promising applications for magnetic relaxation switching sensing, cell and pathogen detection, cell targeting and magnetic resonance imaging. We end with a critical perspective of the field, identifying the main challenges to be overcome, but also the areas where the most promising developments are likely to happen in the coming decades.

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“…Another recent example was the preparation of glycoferrite nanoparticles (superparamagnetic water-soluble coreshell X Fe 2 O 4 (where X = iron (Fe), manganese (Mn), or cobalt (Co)) nanoparticles) with different carbohydrates and carboxylic linkers and functionalized with a fluorescent dye (Texas Red). Additionally, protein G was conjugated to attach well oriented antibodies to obtain immuno-fluorescent-magnetic glycoferrites [97,98]. These multimodal GNPs have allowed for the specific labeling of peripheral blood mononuclear cells (PBMCs) present in blood in a percentage below 2% by fluorescent microscopy and by magnetic resonance imaging [97].…”

Section: Metallic Glyconanoparticlesmentioning

confidence: 99%