Magnetic framework composites for polycyclic aromatic hydrocarbon sequestration

Beta-Glucosidase has been chosen as a model biomolecule to establish a general protocol for binding enzymes on both ferromagnetic and superparamagnetic nano-particles for sensing applications. Using EDC (1-(3-dimethyl-aminopropyl)-3-ethylcarbodiimide) or SMCC (Succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) as coupling agents, we compared two different methods for the fabrication of enzyme-decorated magnetic nanoparticles. We identified the best conditions for the preparation of a responsive bioactive magnetic system comparing different covalent bio-grafting protocols. The enzymatic test has been performed using beta-Glucosidase. The systems were characterized using scanning electron microscopy, infrared spectroscopy, and the enzyme loading was measured by a glucose assay in the presence of the enzyme-decorated magnetic particles. Although the faster response of ferromagnetic particles to the magnetic field, the assay results suggested that the superparamagnetic particles are more efficient carriers. In fact, the best enzymatic activity was measured on superparamagnetic systems that have the further advantage of preventing aggregation induced by the residual magnetization. Hence, beta-Glucosidase coated magnetic nanospheres could provide an attractive system suitable for the cleavage and the rapid evaluation of glycoside levels in natural products, measuring the liberated glucose without the need for specialised instrumentation. Moreover, the magnetic particles allow the subsequent collection of enzymes for further analysis, such as its use in portable fast screening kits or devices.

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“…The advantage of superparamagnetic materials is to prevent uncontrolled aggregation, 28 enabling efficient dispersion in the buffer medium.…”

Section: -Carboxyethylphosphine) This Reactant Is Able To Openmentioning

confidence: 99%

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Riccò¹,

Doherty²,

Falcaro³

2014

j nanosci nanotechnol

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“…[4][5][6][7] In addition, new synergetic properties have been obtained by incorporating functional species into MOFs, further expanding their potential applications. [8][9][10][11][12][13][14][15][16][17] While there are a large number of reports describing different methods for successfully combining exogenous species into MOFs, [18][19][20][21] the encapsulation of nanoparticles is particularly promising due to the innovative functional properties that cannot be obtained from the parent MOFs alone. [22][23][24][25][26] Typically, the encapsulation of nanoparticles is achieved through the introduction of precursors using solution impregnation, gas-phase infiltration or a solid-phase method, and subsequent conversion into the corresponding functional components inside the frameworks.…”

Section: Introductionmentioning

confidence: 99%

Fe₃O₄@HKUST-1 and Pd/Fe₃O₄@HKUST-1 as magnetically recyclable catalysts prepared via conversion from a Cu-based ceramic

et al. 2017

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Nanocomposites obtained by integrating iron oxide magnetic nanoparticles (Fe 3 O 4 ) into a metal-organic framework (HKUST-1 or Cu 3 ĲBTC) 2 , BTC = 1,3,5-benzenetricarboxylate) are synthesized through conversion from a composite of a Cu-based ceramic material and Fe 3 O 4 . In situ small-angle X-ray scattering (SAXS) and wide-angle X-ray scattering (WAXS) measurements reveal that the presence of

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“…Reproduced from [168]; b) Time dependence of the dibenzothiophene capture by Iron oxide nanoparticle@HKUST-1superstructure (in the inset) obtained by spray-drying. Reproduced from[170].…”

mentioning

confidence: 98%

Application of metal and metal oxide nanoparticles@MOFs

Falcaro

Riccò

Yazdi

et al. 2016

Coordination Chemistry Reviews

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520

244

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Magnetic framework composites for polycyclic aromatic hydrocarbon sequestration

Cited by 66 publications

References 35 publications

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Fe₃O₄@HKUST-1 and Pd/Fe₃O₄@HKUST-1 as magnetically recyclable catalysts prepared via conversion from a Cu-based ceramic

Application of metal and metal oxide nanoparticles@MOFs

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Magnetic framework composites for polycyclic aromatic hydrocarbon sequestration

Cited by 66 publications

References 35 publications

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Evaluation of Coupling Protocols to Bind Beta-Glucosidase on Magnetic Nanoparticles

Fe3O4@HKUST-1 and Pd/Fe3O4@HKUST-1 as magnetically recyclable catalysts prepared via conversion from a Cu-based ceramic

Application of metal and metal oxide nanoparticles@MOFs

Contact Info

Product

Resources

About

Fe₃O₄@HKUST-1 and Pd/Fe₃O₄@HKUST-1 as magnetically recyclable catalysts prepared via conversion from a Cu-based ceramic