Magnetic-field-induced orientation of fructose dehydrogenase on iron oxide nanoparticles for enhanced direct electron transfer

Kizling, Michał; Rękorajska, Aleksandra; Krysiński, Paweł; Bilewicz, Renata

doi:10.1016/j.elecom.2018.06.010

Cited by 12 publications

(11 citation statements)

References 18 publications

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“…Recently, the magnetic‐field‐induced orientation of fructose dehydrogenase on iron oxide nanoparticles for enhanced direct electron transfer (DET) has been reported . In this study, the magnetic interactions between the paramagnetic heme groups of fructose dehydrogenase subunit II and superparamagnetic iron oxide nanoparticles enables a suitable orientation of the enzyme molecule and enhance the rate of DET …”

Section: Introductionmentioning

confidence: 95%

“…Electrochemical reactions, including bioelectrocatalytic and electrocatalytic processes, have been reversibly activated and inhibited upon physical translocation and reorientation of different magnetic micro/nano‐species on the electrode surface, particularly using magnetic micro‐and nanoparticles . Recently, the magnetic‐field‐induced orientation of fructose dehydrogenase on iron oxide nanoparticles for enhanced direct electron transfer (DET) has been reported . In this study, the magnetic interactions between the paramagnetic heme groups of fructose dehydrogenase subunit II and superparamagnetic iron oxide nanoparticles enables a suitable orientation of the enzyme molecule and enhance the rate of DET …”

Section: Introductionmentioning

confidence: 97%

“…[16] Recently, the magnetic-field-induced orientation of fructose dehydrogenase on iron oxide nanoparticles for enhanced direct electron transfer (DET) has been reported. [18] In this study, the magnetic interactions between the paramagnetic heme groups of fructose dehydrogenase subunit II and superparamagnetic iron oxide nanoparticles enables a suitable orientation of the enzyme molecule and enhance the rate of DET. [18] Herein, we present the formation of a mesoporous framework resultant from magnetically induced assembly of magnetite nanoparticles decorated with near-monodisperse gold nanoparticles (Fe 3 O 4 À AuNPs) onto indium-tin-oxide (ITO) electrode.…”

Section: Introductionmentioning

confidence: 99%

“…[18] In this study, the magnetic interactions between the paramagnetic heme groups of fructose dehydrogenase subunit II and superparamagnetic iron oxide nanoparticles enables a suitable orientation of the enzyme molecule and enhance the rate of DET. [18] Herein, we present the formation of a mesoporous framework resultant from magnetically induced assembly of magnetite nanoparticles decorated with near-monodisperse gold nanoparticles (Fe 3 O 4 À AuNPs) onto indium-tin-oxide (ITO) electrode. Although different approaches for the formation of mesoporous nanostructures at electrode surfaces have been developed, [19][20][21] the magnetic field-based method has been shown as one of the simplest and most efficient tool for this application.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Behavior of Cytochrome C Immobilized in a Magnetically Induced Mesoporous Framework

et al. 2019

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Protein immobilization on electrode surfaces remains a technological challenge and is decisive in enhancing biological properties with simplified operational steps. In view of proposing a new direction for protein immobilization, we report the development of a mesoporous framework resulting from the magnetically induced assembly of magnetite nanoparticles decorated with near‐monodisperse gold nanoparticles onto an indium‐tin‐oxide electrode. With an electrochemically active surface area of about 72 cm2 and pores with an average size of 31±1 nm, this magnetically induced mesoporous framework is suitable for the immobilization of small redox proteins. As a proof‐of‐concept, we show the electrochemical process of cytochrome c, where the mesoporous electrode provided a fourfold enhancement for the faradaic currents in comparison to standard polycrystalline gold electrodes.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical Behavior of Cytochrome C Immobilized in a Magnetically Induced Mesoporous Framework

et al. 2019

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“…Many papers present effective bioelectrocatalytical processes, where several mono- and multi-cofactor redox enzymes [7], such as cellobiose dehydrogenase [5, 8, 9], horseradish peroxidase, superoxide dismutase, fructose dehydrogenase [10–12], blue multicopper oxidases (MCOs) [13, 14], and human sulfite oxidase [15], have been immobilized onto nanostructured electrodes. Despite the large interest in AuNPs, there are only few reports about the influence of the size (e.g., diameter) and shape of the NPs on the enzymatic reactions occurring at the electrodes, sometimes improving the ET rate or changing the mechanism of the bioelectrocatalytic process [16, 17].…”

Section: Introductionmentioning

confidence: 99%

The influence of the shape of Au nanoparticles on the catalytic current of fructose dehydrogenase

et al. 2019

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Graphite electrodes were modified with triangular (AuNTrs) or spherical (AuNPs) nanoparticles and further modified with fructose dehydrogenase (FDH). The present study reports the effect of the shape of these nanoparticles (NPs) on the catalytic current of immobilized FDH pointing out the different contributions on the mass transfer–limited and kinetically limited currents. The influence of the shape of the NPs on the mass transfer–limited and the kinetically limited current has been proved by using two different methods: a rotating disk electrode (RDE) and an electrode mounted in a wall jet flow-through electrochemical cell attached to a flow system. The advantages of using the wall jet flow system compared with the RDE system for kinetic investigations are as follows: no need to account for substrate consumption, especially in the case of desorption of enzyme, and studies of product-inhibited enzymes. The comparison reveals that virtually identical results can be obtained using either of the two techniques. The heterogeneous electron transfer (ET) rate constants (kS) were found to be 3.8 ± 0.3 s−1 and 0.9 ± 0.1 s−1, for triangular and spherical NPs, respectively. The improvement observed for the electrode modified with AuNTrs suggests a more effective enzyme-NP interaction, which can allocate a higher number of enzyme molecules on the electrode surface. Graphical abstractThe shape of gold nanoparticles has a crucial effect on the catalytic current related to the oxidation of D-(-)-fructose to 5-keto-D-(-)-fructose occurring at the FDH-modified electrode surface. In particular, AuNTrs have a higher effect compared with the spherical one. Electronic supplementary materialThe online version of this article (10.1007/s00216-019-01944-6) contains supplementary material, which is available to authorized users.

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Magnetically Stimulated Bio‐ and Electrochemical Systems: State‐of‐the‐Art, Applications, and Future Directions

et al. 2023

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Magnetically stimulated bio‐and electrochemical systems have gained increasing importance in recent years due to their enhanced sensitivity and selectivity, improved spatial control, and ability to operate in complex environments, offering significant advantages over conventional systems. Essentially, this review provides a comprehensive and informative survey about the state‐of‐art as well as the recent efforts and applications made in the past decade, with a focus on new functional magnetic materials and nano‐architectonic interfaces that have led to promising advances in biosensors, biofuel cells, and their integration into devices. Future directions and perspectives are also discussed.

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Magnetic-field-induced orientation of fructose dehydrogenase on iron oxide nanoparticles for enhanced direct electron transfer

Cited by 12 publications

References 18 publications

Electrochemical Behavior of Cytochrome C Immobilized in a Magnetically Induced Mesoporous Framework

Electrochemical Behavior of Cytochrome C Immobilized in a Magnetically Induced Mesoporous Framework

The influence of the shape of Au nanoparticles on the catalytic current of fructose dehydrogenase

Magnetically Stimulated Bio‐ and Electrochemical Systems: State‐of‐the‐Art, Applications, and Future Directions

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