With the rapid development of nanotechnology and widespread use of nanoproducts, concerns have arisen regarding the ecotoxicity of these materials. In this paper, the photosynthetic toxicity and oxidative damage induced by nanoFe 3 O 4 on a model organism, Chlorella vulgaris (C. vulgaris) in aquatic environment, were studied. The results showed that nano-Fe 3 O 4 was toxic to C. vulgaris and affected its content of chlorophyll a, malonaldehyde and glutathione, CO 2 absorption, net photosynthetic rate, superoxide dismutase activity and inhibition of hydroxyl radical generation. At higher concentrations, compared with the control group, the toxicity of nano-Fe 3 O 4 was significantly different. It suggested that nano-Fe 3 O 4 is ecotoxic to C. vulgaris in aquatic environment.