Magnetic Fe3O4 nanoparticle catalyzed chemiluminescence for detection of nitric oxide in living cells

Abstract

Direct and real-time measurement of nitric oxide (NO) in biological media is very difficult due to its transient nature. Fe3O4 nanoparticles (nanoFe3O4) because of their unique catalytic activities have attracted much attention as catalysts in a variety of organic and inorganic reactions. In this work, we have developed a magnetic Fe3O4 nanoparticle-based rapid-capture system for real-time detection of cellular NO. The basic principle is that the nanoFe3O4 can catalyze the decomposition of H2O2 in the system to generate superoxide anion (O2 (·-)) and the O2 (·-) can serve as an effective NO(·) trapping agent yielding peroxynitrite oxide anion, ONOO(-). Then the concentration of NO in cells can be facilely determined via peroxynitrite-induced luminol chemiluminescence. The linear range of the method is from 10(-4) to 10(-8) mol/L, and the detection of limit (3σ, n = 11) is as low as 3.16 × 10(-9) mol/L. By using this method, the NO concentration in 0.1 and 0.5 mg/L LPS-stimulated BV2 cells was measured as 4.9 and 11.3 μM, respectively. Surface measurements by synchrotron X-ray photoelectron spectroscopy (SRXPS) and scanning transmission X-ray microscopy (STXM) demonstrate the catalytic mechanism of the nanoFe3O4-based system is that the significantly excess Fe(II) exists on the surface of nanoFe3O4 and mediates the rapid heterogeneous electron transfer, thus presenting a new Fe2O3 phase on the surface.

Multiscale Synchrotron-Based Imaging Analysis for the Transfer of PEGylated Gold Nanoparticles In Vivo

“…Synchrotron radiation (SR), because of its small beam size, low divergence, high photon flux, and linearly polarized nature, is an ideal source for the nondestructive imaging analysis. , In the last decades, synchrotron radiation have developed various powerful imaging techniques, such as microbeam XRF (μXRF), , nano and microcomputed tomography (nano and micro-CT), − scanning transmission X-ray microscopy (STXM) imaging, , Fourier transform infrared (FTIR) spectroscopy imaging, etc., perform rapid, nondestructive, and label-free analysis of specimens based on principles of absorption, phase contrast, diffraction, fluorescence, etc., The synchrotron-based XRF, with a nondestructive character, can probe trace elements in biological samples with high sensitivity (sub mg·kg –1 ) and high spatial resolution (submicrometer). , FTIR microspectroscopy imaging is the technique combination of light microscopy and infrared spectroscopy, which can simultaneously provide information on structural details and molecular chemistry in samples. , The main advantage of synchrotron infrared over a traditional source is its 100–1000 times brilliance that offers operating wavelength from near-to-visible IR (wavelength >500 cm –1 ) to far-visible IR (wavelength >4000 cm –1 ) with a high signal-to-noise ratio at high spatial resolution (2–10 μm, near diffraction limit) in a short collecting time (∼minutes) . For instance, Findlay et al showed FTIR images of plaque cores (1620–1630 cm –1 ), lipid membranes (2844–2857 cm –1 ), and creatine crystals (1300–1311 cm –1 ) in a hippocampal tissue, which could provide important information in biomedicine.…”

“…8 Synchrotron radiation (SR), because of its small beam size, low divergence, high photon flux, and linearly polarized nature, is an ideal source for the nondestructive imaging analysis. 9,10 In the last decades, synchrotron radiation have developed various powerful imaging techniques, such as microbeam XRF (μXRF), 10,11 nano and microcomputed tomography (nano and micro-CT), 12−14 scanning transmission X-ray microscopy (STXM) imaging, 15,16 Fourier transform infrared (FTIR) spectroscopy imaging, etc., perform rapid, nondestructive, and label-free analysis of specimens based on principles of absorption, phase contrast, diffraction, fluorescence, etc., The synchrotron-based XRF, with a nondestructive character, can probe trace elements in biological samples with high sensitivity (sub mg•kg −1 ) and high spatial resolution (submicrometer). 10,17 FTIR microspectroscopy imaging is the technique combination of light microscopy and infrared spectroscopy, which can simultaneously provide information on structural details and molecular chemistry in samples.…”

Surface enhanced Raman spectroscopic studies on the adsorption behaviour of nitric oxide on a Ru covered Au nanoparticle film

“…4,5 From a medical point of view as well as an industrial perspective, the detection and determination of NO in a rapid, highly sensitive way is an important and signicant challenge. For many years, various techniques have been developed for the detection of NO, such as uorescence, 6,7 chemiluminescence, 8 electron spin resonance spectrophotometry, 9 colorimetry 10 and electrochemical methods. 11,12 Among these methods, uorescence and electrochemical techniques have become powerful tools for the investigation of this radical species.…”

Pt NPs catalyzed chemiluminescence method for Hg2+ detection based on a flow injection system

“…In recent years, luminol CL systems that utilize NPs as catalysts and amplify CL signals have attracted many researchers, due to their high sensitivity or selectivity. [18][19][20][21][22] Various NPs, such as semiconductors, metals, metal oxides have proved to be effective enhancers for different CL systems and have been widely employed to detect heavy metal ions. [23] Up to now, Au NPs have been successfully employed as catalysts and have been used to detect As 3+ and Hg 2+ based on CL method.…”