Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins

Tang, Qiuhan; Zhao, Rui; Lü, Qing; Qing, Guangyan

doi:10.1007/s11595-019-2113-z

Cited by 1 publication

(1 citation statement)

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“…On the other hand, its a nity toward phosphoric group via Ti-O-P coordination has gained enormous application in phosphoprotein-enrichment. In addition, the lower Lewis acidity and higher hydrophilicity of aminofunctionalized TiO 2 (NH 2 -TiO 2 ) than TiO 2 show strong a nity with phosphoproteins, then modifying -NH 2 onto TiO 2 has gained enormous attraction [20]. Based on the prominent photocatalytic performance and strong speci c recognition utility between NH 2 -TiO 2 and phosphate group, if or not a label-free ECLbiosensor may be developed to detect the activity of PKA?…”

Section: Introductionmentioning

confidence: 99%

Phosphopeptide-bridged NH2-TiO2-mediated carbon dots self-enhancing and electrochemiluminescence microsensors for label-free protein kinase A detection

Guo,

Yue,

Ning

et al. 2024

Preprint

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A novel electrochemiluminescence (ECL) method was developed for analyzing protein kinase A (PKA) ultra-sensitively based on amidated nano-titanium (NH2-TiO2) embellished carbon dots (Mg@N-CDs) fluorescent probe, which integrated the target recognition and ECL-signal enhancement. The Cys-labeled kemptides were employed to build a serine-rich synthetic substrate-heptapeptide (Cys-kemptide) on the Au-electrode surface. Then, the PKA-induced biosensor was triggered as a signal switch to introduce the large amounts of TiO2 decorated Mg@N-CDs nanohybrid (Ti@NMg-CDs) into AuE/Cys-phosphopeptides for signal output. In particular, the presence of PKA could induce the formation of Cys-phosphopeptides by the catalytic reaction between specific substrate (kemptide) and PKA, which could act as an initiator to link the Ti@NMg-CDs according to the bridge interactions Ti-O-P. By this way, multiple Cys-phosphopeptides were adsorbed onto a single Ti@NMg-CDs and the Ti@NMg-CDs not only provided the high specific selectivity but also large surface area, as well as unprecedented high ECL efficiency. Using this PKA-induced enhanced sensor, the limit-of-detection of the PKA was 4.89 × 10− 4 U/mL (S/N = 3). The proposed ECL biosensor was also universally applicable for the screening of PKA inhibitors and the determining of other kinases activity. Our sensing-system has excellent performance of specificity and the screening of kinase inhibitors, as well as it will inspire future effort on clinical diagnostics and new drugs discovery.

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Section: Introductionmentioning

confidence: 99%