Magnetic enzyme reactors for isolation and study of heterogeneous glycoproteins

Korecká, Lucie; Ježová, Jana; Bı́lková, Zuzana; Beneš, Milan J.; Horák, Daniel; Hradcová, Olga; Slováková, Marcela; Viovy, Jean‐Louis

doi:10.1016/j.jmmm.2005.02.030

Cited by 24 publications

(14 citation statements)

References 15 publications

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“…The reaction conditions for protein fragmentation by trypsin reactors were optimized (Table 3), following an approach similar to this described by Korecká et al [51,52]. Briefly, the type of buffer, ions, pH, temperature, E:S ratio, digestion time, surfactant, and K M , as well as the composition of the storage solution were varied.…”

Section: Characterization Of Proteolytic Microreactorsmentioning

confidence: 99%

Functionalized magnetic micro‐ and nanoparticles: Optimization and application to μ‐chip tryptic digestion

et al. 2006

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The preparation of an easily replaceable protease microreactor for micro-chip application is described. Magnetic particles coated with poly(N-isopropylacrylamide), polystyrene, poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate), poly(glycidyl methacrylate), [(2-amino-ethyl)hydroxymethylen]biphosphonic acid, or alginic acid with immobilized trypsin were utilized for heterogeneous digestion. The properties were optimized, with the constraint of allowing immobilization in a microchannel by a magnetic field gradient. To obtain the highest digestion efficiency, sub-micrometer spheres were organized by an inhomogeneous external magnetic field perpendicularly to the direction of the channel. Kinetic parameters of the enzyme reactor immobilized in micro-chip capillary (micro-chip immobilized magnetic enzyme reactor (IMER)) were determined. The capability of the proteolytic reactor was demonstrated by five model (glyco)proteins ranging in molecular mass from 4.3 to 150 kDa. Digestion efficiency of proteins in various conformations was investigated using SDS-PAGE, HPCE, RP-HPLC, and MS. The compatibility of the micro-chip IMER system with total and limited proteolysis of high-molecular-weight (glyco)proteins was confirmed. It opens the route to automated, high-throughput proteomic micro-chip devices.

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Section: Characterization Of Proteolytic Microreactorsmentioning

confidence: 99%

Functionalized magnetic micro‐ and nanoparticles: Optimization and application to μ‐chip tryptic digestion

et al. 2006

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“…Magnetic particles provide a universal system with additional convenience, consistency, stability, ease of handling and exceptional flexibility compared to standard chromatography resinos. Hydrophilic macroporous bead cellulose is carrier with a high surface area, with easily modified OHÀ groups for covalent ligand attachment, minimal nonspecific binding for common protein and peptide molecules, no tendency to aggregate and excellent flow characteristics [13]. Such characteristics of carrier combined with improved immobilization strategy could significantly improve the conditions for enzyme catalysis of azo dyes degradation and other industrial applications.…”

mentioning

confidence: 99%

Laccase immobilized on magnetic carriers for biotechnology applications

Rotkova

Šuláková²,

Korecká

et al. 2009

Journal of Magnetism and Magnetic Materials

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“…Various enzymes including glucoamylase (Bahar & Celebi, 1999), cytochrome C oxidase (Cuyper & Joniau, 1992), β-lactamase (Gao et al, 2003), chymotrypsin (Izmaĭlov et al, 2000, Rebelo et al, 2010, alcohol dehydrogenase , glucose oxidase, galactose oxidase, urease (Varlan & Sansen Ann Van, 1996), neuramidinase (Bilkova et al, 2002), papain (Korecká et al, 2005), Dnase (Rittich et al, 2002), Rnase (Horák et al, 2001), etc. can be immobilized via chemical bonds on coated or embedded magnetic particles.…”

Section: Property Improvement Of the Immobilized Enzymes On Magnementioning

confidence: 99%