Magnetic core mesoporous silica nanoparticles doped with dacarbazine and labelled with 99mTc for early and differential detection of metastatic melanoma by single photon emission computed tomography

Abstract

Cancer is responsible for more than 12% of all causes of death in the world, with an annual death rate of more than 7 million people. In this scenario melanoma is one of the most aggressive ones with serious limitation in early detection and therapy. In this direction we developed, characterized and tested in vivo a new drug delivery system based on magnetic core-mesoporous silica nanoparticle that has been doped with dacarbazine and labelled with technetium 99 m to be used as nano-imaging agent (nanoradiopharmaceutical) for early and differential diagnosis and melanoma by single photon emission computed tomography. The results demonstrated the ability of the magnetic core-mesoporous silica to be efficiently (>98%) doped with dacarbazine and also efficiently labelled with 99mTc (technetium 99 m) (>99%). The in vivo test, using inducted mice with melanoma, demonstrated the EPR effect of the magnetic core-mesoporous silica nanoparticles doped with dacarbazine and labelled with technetium 99 metastable when injected intratumorally and the possibility to be used as systemic injection too. In both cases, magnetic core-mesoporous silica nanoparticles doped with dacarbazine and labelled with technetium 99 metastable showed to be a reliable and efficient nano-imaging agent for melanoma.

Molecular and Cellular Risk Assessment of Healthy Human Cells and Cancer Human Cells Exposed to Nanoparticles

“…In order to evaluate the cell viability we performed the MTT assay testing a dose of 20 ug/mL. This dose has been used by our group in a variety of studies in vitro (22,(45)(46). However, there is a lack of evidence related to the toxic effects of this dose.…”

“…NPs have been developed for disease diagnosis and targeted drug delivery systems [63,64]. The human tumor cells lines used in this work study are under constant investigation by other groups that have contributed to information about the behavior, mechanisms and treatment efficacy [45,[58][59][60][61][62][63][64][65][66][67][68][69]. Nanomedicine, emerge as a promising platform for theranostic strategies [65].…”

The Effect of Nanosystems on ATP-Binding Cassette Transporters: Understanding the Influence of Nanosystems on Multidrug Resistance Protein-1 and P-glycoprotein

“…Mesoporous silica nanoparticles (MSN) are structures composed of silica organized in a well-ordered internal mesopores with large pore volume and surface area, presenting properties such as tunable size and shape, cost-effective fabrication, high drug loading capability, easy surface modification, well-tolerated in vitro and in vivo [45] and already applied in many therapeutic and diagnostic formulations for anticancer activity [65,66].…”

SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay

“…Moreover, Huang X. and collaborators assessed the effect of mesoporous silica nanoparticles on tumour proliferation while using a model of nude mice xenografted with A375 cells [51]. Additionally, the following reasons led us to the selection of this cell line: (i) both superparamagnetic iron and silica nanoparticles proved to be reliable nanoplatforms for the detection and treatment of different cancers, including melanoma [40,[52][53][54][55] and (ii) A375 melanoma cell line presents the characteristics of the human genitor and possess B-RAF and CDKN2 mutations, which are typical of cutaneous melanoma, representing an eligible candidate for the development of in vivo models [56].…”