Magnetic control of the DNA synthesis

Бучаченко, А. Л.; Орлов, А. А.; Kuznetsov, Dmitry A.; Breslavskaya, N. N.

doi:10.1016/j.cplett.2013.07.056

Cited by 35 publications

(36 citation statements)

References 16 publications

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“…The first process is an increase of ATP production. This is analogous to that observed for 25 Mg ions and is specific for different phosphotransferases in catalytic process that occur for magnesium, zinc, and calcium [8]. Thus all energy dependent processes that directly or indirectly occur due to the effects of magnetic nuclei and their modu lation depending on the object can be both up or down regulated.…”

Section: Resultssupporting

confidence: 66%

“…The topology of the active cen ter and changes of conformation (particularly at pH value alteration) in enzyme molecules are of great importance [7]. As well, MIE was found for other phosphate transport reactions, which are catalyzed by pyruvate kinase and phosphoglycerate kinase [8]. It is known that the reaction of ATP synthesis is catalyzed not only by magnesium ions but also by calcium and zinc ions.…”

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confidence: 99%

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The magnetic isotope effect as a tool for apoptosis modulation in leukemic cells

Орлова

Полозников

Орлов

2014

Moscow Univ. Chem. Bull.

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The magnetic isotope effect (MIE) was discovered for isotopes of carbon as a relationship between the reaction rate (or probability of molecule formation) and nuclear spin, its projection, magnetic moment, and energy of electron nuclear (superfine) interaction [1]. MIE leads to isotope fractionation in dependence on their magnetic moment. This effect was later discovered [2] to occur for many pairs and triads of isotopes:Abstract-Lymphocytes from healthy donors and leukemic cells of patients with acute B lymphoblastic leu kemia (BALL 1) and acute myeloid leukemia were exposed to nanoparticles bearing magnetic (Zn 67) and nonmagnetic (total isotope pool) nuclei of zinc. The values of the corresponding magnetic isotope effects determined as the ratio of LD 50 magnitudes of preparations with magnetic and nonmagnetic zinc isotopes were 0, 3.5, and 1.5. Morphological studies using confocal and fluorescence microscopy showed apoptotic death of cells with the preparations; as well, there was an increase in the cell aggregation and better aggrega tion of nanoparticles in the case of 67 Zn NP, which resulted in a decrease of cytotoxicity. However the mag netic isotope effect was observed even in the case of aggregation.

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Section: Resultssupporting

confidence: 66%

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confidence: 99%

The magnetic isotope effect as a tool for apoptosis modulation in leukemic cells

Орлова

Полозников

Орлов

2014

Moscow Univ. Chem. Bull.

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“…For example, intracellular ATP content increased 3.5 times after 1 h of exposure to the low static magnetic field (17 mT) in E. coli bacteria [Filipič et al, ]. The external weak magnetic field application enhances magnetic magnesium isotope efficiency in enzymatic synthesis of ATP [Buchachenko and Kuznetsov, ; Buchachenko et al, ]. Similar magnetic field dependencies were obtained for the ATP pool in E. coli bacteria, cultivated in a static magnetic field 0.8–16 mT for 8 h with magnesium isotopes [Letuta et al, ; Avdeeva and Letuta, ].…”

Section: Resultsmentioning

confidence: 89%

Enzymatic mechanisms of biological magnetic sensitivity

Летута

Berdinskiy

Udagawa

et al. 2017

Bioelectromagnetics

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Primary biological magnetoreceptors in living organisms is one of the main research problems in magnetobiology. Intracellular enzymatic reactions accompanied by electron transfer have been shown to be receptors of magnetic fields, and spin-dependent ion-radical processes can be a universal mechanism of biological magnetosensitivity. Magnetic interactions in intermediate ion-radical pairs, such as Zeeman and hyperfine (HFI) interactions, in accordance with proposed strict quantum mechanical theory, can determine magnetic-field dependencies of reactions that produce biologically important molecules needed for cell growth. Hyperfine interactions of electrons with nuclear magnetic moments of magnetic isotopes can explain the most important part of biomagnetic sensitivities in a weak magnetic field comparable to the Earth's magnetic field. The theoretical results mean that magnetic-field dependencies of enzymatic reaction rates in a weak magnetic field that can be independent of HFI constant a, if H << a, and are determined by the rate constant of chemical transformations in the enzyme active site. Both Zeeman and HFI interactions predict strong magnetic-field dependence in weak magnetic fields and magnetic-field independence of enzymatic reaction rate constants in strong magnetic fields. The theoretical results can explain the magnetic sensitivity of E. coli cell and demonstrate that intracellular enzymatic reactions are primary magnetoreceptors in living organisms. Bioelectromagnetics. 38:511-521, 2017. © 2017 Wiley Periodicals, Inc.

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“…The activity was found to depend on the nuclear magnetic moment: the ions 25 Mg 2+ and 67 Zn 2+ with magnetic nuclei 25 Mg and 67 Zn strongly suppress enzymatic activity of polymerase (Fig. ) [Buchachenko et al, ,].…”

Section: Introductionmentioning

confidence: 99%

“…Nucleophilic mechanism of the nucleotide attachment to the growing DNA macromolecule implies direct addition of 3′O − ion of the ribose ring to the P α atom of the incoming nucleotide phosphate and simultaneous release of pyrophosphate anion [Steitz and Steitz, ; Steitz, ]. Radical mechanism, coexisting with nucleophilic one, suggests electron transfer from 3′O − ion to the Mg(H 2 O) n 2+ ion as a key, primary reaction (for details concerning this mechanism see [Buchachenko et al, ,]). Radical mechanism of the DNA synthesis is reliably validated; moreover, discovery of magnetic effects on the DNA synthesis simultaneously proves commonly accepted nucleophilic mechanism.…”

Section: Introductionmentioning

confidence: 99%

Why magnetic and electromagnetic effects in biology are irreproducible and contradictory?

Бучаченко

2015

Bioelectromagnetics

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The main source of magnetic and electromagnetic effects in biological systems is now generally accepted and demonstrated in this paper to be radical pair mechanism which implies pairwise generation of radicals in biochemical reactions. This mechanism was convincingly established for enzymatic adenosine triphosphate (ATP) and desoxynucleic acid (DNA) synthesis by using catalyzing metal ions with magnetic nuclei ((25)Mg, (43)Ca, (67)Zn) and supported by magnetic field effects on these reactions. The mechanism, is shown to function in medicine as a medical remedy or technology (trans-cranial magnetic stimulation, nuclear magnetic control of the ATP synthesis in heart muscle, the killing of cancer cells by suppression of DNA synthesis). However, the majority of magnetic effects in biology remain to be irreproducible, contradictory, and enigmatic. Three sources of such a state are shown in this paper to be: the presence of paramagnetic metal ions as a component of enzymatic site or as an impurity in an uncontrollable amount; the property of the radical pair mechanism to function at a rather high concentration of catalyzing metal ions, when at least two ions enter into the catalytic site; and the kinetic restrictions, which imply compatibility of chemical and spin dynamics in radical pair. It is important to keep in mind these factors to properly understand and predict magnetic effects in magneto-biology and biology itself and deliberately use them in medicine.

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Magnetic control of the DNA synthesis

Cited by 35 publications

References 16 publications

The magnetic isotope effect as a tool for apoptosis modulation in leukemic cells

The magnetic isotope effect as a tool for apoptosis modulation in leukemic cells

Enzymatic mechanisms of biological magnetic sensitivity

Why magnetic and electromagnetic effects in biology are irreproducible and contradictory?

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