Magnetic carbon nanotube labelling for haematopoietic stem/progenitor cell tracking

Gul, Hilal; Lu, Wei‐Bing; Xu, Peng; Jin, Xing; Chen, Jie

doi:10.1088/0957-4484/21/15/155101

Cited by 35 publications

(28 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Signals from the environment can, for example, instead of stimulating the onset of a cellular differentiation, induce the process of cell division in order to self-renew the pool of undifferentiated cells. 172 Both processes -cell proliferation and differentiationare based mainly on the right nanotopography surroundings and cellular microenvironment composition, and on the presence of appropriate substrates.…”

Section: Stem Cells and Carbon Nanotubesa New Tissuementioning

confidence: 99%

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Tonelli¹,

Santos²,

Gomes³

et al. 2012

IJN

View full text Add to dashboard Cite

Abstract:In recent years, significant progress has been made in organ transplantation, surgical reconstruction, and the use of artificial prostheses to treat the loss or failure of an organ or bone tissue. In recent years, considerable attention has been given to carbon nanotubes and collagen composite materials and their applications in the field of tissue engineering due to their minimal foreign-body reactions, an intrinsic antibacterial nature, biocompatibility, biodegradability, and the ability to be molded into various geometries and forms such as porous structures, suitable for cell ingrowth, proliferation, and differentiation. Recently, grafted collagen and some other natural and synthetic polymers with carbon nanotubes have been incorporated to increase the mechanical strength of these composites. Carbon nanotube composites are thus emerging as potential materials for artificial bone and bone regeneration in tissue engineering.

show abstract

Section: Stem Cells and Carbon Nanotubesa New Tissuementioning

confidence: 99%

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Tonelli¹,

Santos²,

Gomes³

et al. 2012

IJN

View full text Add to dashboard Cite

show abstract

“…FITC-mCNT were synthesized as previously described (Gul et al 2010) and cells were exposed to FITC-mCNT (40 lg/ml) in the presence of both rotating and static magnetic fields for 10 min each (see Fig. 1a).…”

Section: Resultsmentioning

confidence: 99%

“…FITClabelled mCNT were synthesized according to Gul et al (2010). In brief, mCNT were oxidized to form carboxylic acid groups on the surface.…”

Section: Preparation Of Fitc-mcntmentioning

confidence: 99%

“…Magnetic field-driven delivery method was applied as described previously (Gul et al 2010). Briefly, culture dishes vertically placed into a beaker containing 50 ml serum-free IMDM media with FITCmCNT or mCNT (40 lg/ml) and then beaker was placed on a magnetic stirrer at 1,200 rpm for 10 min (refer to Fig.…”

Section: Cell Viabilitymentioning

confidence: 99%

“…Therefore, research has focused on the development of elongated MNP such as carbon nanotubes (CNT). We and others have been previously reported the efficient magnetic CNT (mCNT)-based molecular delivery into hard-to-transfect cells including primary mammalian neurons and hematopoietic stem/progenitor cells (HSPC) (Cai et al 2005;Gul et al 2010). In this study, we further investigate the efficiency and cytotoxicity of FITC-mCNT uptake by human monocytic leukemia THP-1 cells for enhancing cellbased cancer gene therapy protocols.…”

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Efficient and rapid uptake of magnetic carbon nanotubes into human monocytic cells: implications for cell-based cancer gene therapy

Gul-Uludag

et al. 2012

Biotechnol Lett

Self Cite

View full text Add to dashboard Cite

Monocyte-based gene therapies in cancer have been hampered by either the resistance of these cells to non-viral molecular delivery methods or their poor trafficking to the tumor site after their ex vivo manipulations. Magnetic nanoparticles (MNP)-loaded genetically engineered monocytes can efficiently delivered to tumor site by external magnetic field, but they are not ideal delivery tools due to their spherical shape. Hence, we have investigated the cellular uptake efficiency and cytotoxicity of fluorescein isothiocyanate (FITC)-labelled magnetic carbon nanotubes (FITC-mCNT) in human monocytic leukemia cell line THP-1 for application in cell-based gene therapy against cancer. Uptake of FITC-mCNT into THP-1 cells reached 100% only 1 h after the delivery. Confocal imaging confirmed that FITC-mCNT entered the cell cytoplasm and even into the nucleus. FITC-mCNT uptake did not compromise cell viability. This delivery system might therefore enhance cell-based cancer gene therapies.

show abstract

Protamine Functionalized Single‐Walled Carbon Nanotubes for Stem Cell Labeling and In Vivo Raman/Magnetic Resonance/Photoacoustic Triple‐Modal Imaging

Wang

et al. 2012

Adv Funct Materials

124

View full text Add to dashboard Cite

Stem cells have shown great potential in regenerative medicine and attracted tremendous interests in recent years. Sensitive and reliable methods for stem cell labeling and in vivo tracking are thus urgently needed. Here, a novel approach to label human mesenchymal stem cells (hMSCs) with single‐walled carbon nanotubes (SWNTs) for in vivo tracking by triple‐modal imaging is presented. It is shown that polyethylene glycol (PEG) functionalized SWNTs conjugated with protamine (SWNT‐PEG‐PRO) exhibit extremely efficient cell entry into hMSCs, without affecting their proliferation and differentiation. The strong inherent resonance Raman scattering of SWNTs is used for in vitro and in vivo Raman imaging of SWNT‐PEG‐PRO‐labeled hMSCs, enabling ultrasensitive in vivo detection of as few as 500 stem cells administrated into mice. On the other hand, the metallic catalyst nanoparticles attached on nanotubes can be utilized as the T2‐contrast agent in magnetic resonance (MR) imaging of SWNT‐labeled hMSCs. Moreover, in vivo photoacoustic imaging of hMSCs in mice is also demonstrated. The work reveals that SWNTs with appropriate surface functionalization have the potential to serve as multifunctional nanoprobes for stem cell labeling and multi‐modal in vivo tracking.

show abstract

Magnetic carbon nanotube labelling for haematopoietic stem/progenitor cell tracking

Cited by 35 publications

References 38 publications

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Carbon nanotube interaction with extracellular matrix proteins producing scaffolds for tissue engineering

Efficient and rapid uptake of magnetic carbon nanotubes into human monocytic cells: implications for cell-based cancer gene therapy

Protamine Functionalized Single‐Walled Carbon Nanotubes for Stem Cell Labeling and In Vivo Raman/Magnetic Resonance/Photoacoustic Triple‐Modal Imaging

Contact Info

Product

Resources

About