Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation

Abstract

Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation have been prepared. They were obtained by a " post-magnetization" procedure involving a simple treatment of the various affinity gels with magnetic ferrofluid. The magnetic biospecific adsorbents tested include magnetic protein A-Sepharose for isolation of IgG antibodies, magnetic human serum albumin (HSA)-Sepharose for anti-HSA isolation, and magnetic 2',5'-ADP for isolation of glucose-6-phosphate dehydrogenase from baker's yeast and hemolyzates of human red blood cells. For the latter enzyme, a 11,000-fold purification was achieved in one step.

Using thiophilic magnetic beads in purification of antibodies from human serum

“…The advent of magnetic affinity separation techniques has opened up a new approach to antibody purification [12][13][14][15]. The rapid process of magnetic separation and specific fractionation of affinity chromatography are combined by this technology.…”

Hybrid Monoliths for Magnetically‐Driven Protein Separations

“…With the rapid development of nanotechnology, iron oxide magnetic nanoparticles (MNPs) are one of the most explored smart material in a wide range of applications including magnetic resonance imaging, drug delivery, immobilization of biomolecules and bioseparations . In particular, MNPs can be combined with various polymers and functionalized with ligands commonly employed in chromatographic methods, leading to nano and micro absorbents suitable for Ab purification . The combination of hydrogels or cryogels with MNPs has also been leading to different magnetic macroporous structures with interconnected pores in the micrometer range .…”

“…Macroporous monoliths based on chitosan, agarose, dextran and PVA, with and without MNPs embedded, were prepared by combining freezing and lyophilization processes . Conversely to the traditional procedures, in this work the natural polymers were not chemically modified but physically entrapped by the polymeric network built from glycidyl methacrylate (GMA), acrylamide and bisacrylamide, that worked as monomers and crosslinking agents, respectively. This strategy assures the biodegradability of the monoliths, since the natural polymers remain unchanged, and offer a sustainable solution for robust materials processing.…”

Potential of magnetically responsive (nano)biocomposites

“…So, in addition to the main currently used strategies focused on specific development of target magnetic structures, alternative strategies leading to the formation of interesting magnetically responsive materials have been developed, which are based on postmagnetization of already existing diamagnetic (''non-magnetic'') or paramagnetic (''weakly'' magnetic) particulate materials found in nature or prepared in the laboratory or industry. The term ''postmagnetization'' and the whole process of magnetic modification of nonmagnetic materials was invented by Mosbach 5,6 at the end of the 70's and beginning of the 80's of the 20th century, when gel particles for column affinity chromatography were magnetically modified using an appropriate ferrofluid (magnetic fluid); such a modification led to the preparation of magnetic derivatives with unaltered biospecificity when applied to general ligand affinity chromatography studies.…”

“…2 Alternatively non-magnetic materials can be modified by erbium ions which preserve their exceptionally high atomic magnetic dipole moment in various chemical structures 7 or by co-entrapment of nonmagnetic and magnetic particles in a gel material. 8 Postmagnetization can be applied to a broad variety of inorganic materials (e.g., clays 9,10 ), activated charcoal, [11][12][13] synthetic polymer particles, 14 biopolymer particles, 5,6 waste plant materials, 15,16 microbial cell walls, 17 whole microbial and algae cells [18][19][20] and many others.…”