Magnetic bead-quantum dot assay for detection of a biomarker for traumatic brain injury

Kim, Chloe; Searson, Peter C.

doi:10.1039/c5nr05608j

Cited by 37 publications

(33 citation statements)

References 41 publications

(154 reference statements)

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“…[29][30][31][32][33] Researchers previously developed a multiplex immunoassay system for lung cancer biomarkers using this integration. 29 Additionally H7 via multilayer QD labeling and magnetic beads was demonstrated to be feasible for detecting Escherichia coli O157.…”

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confidence: 99%

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Zhang

Fan

et al. 2017

IJN

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Background Accurate and reliable blood grouping is essential for safe blood transfusion. However, conventional methods are qualitative and use only single-antigen detection. We overcame these limitations by developing a simple, quantitative, and multiplexed detection method for blood grouping using quantum dots (QDs) and magnetic beads. Methods In the QD fluorescence assay (QFA), blood group A and B antigens were quantified using QD labeling and magnetic beads, and the blood groups were identified according to the R value (the value was calculated with the fluorescence intensity from dual QD labeling) of A and B antigens. The optimized performance of QFA was established by blood typing 791 clinical samples. Results Quantitative and multiplexed detection for blood group antigens can be completed within 35 min with more than 10 5 red blood cells. When conditions are optimized, the assay performance is satisfactory for weak samples. The coefficients of variation between and within days were less than 10% and the reproducibility was good. The ABO blood groups of 791 clinical samples were identified by QFA, and the accuracy obtained was 100% compared with the tube test. Receiver-operating characteristic curves revealed that the QFA has high sensitivity and specificity toward clinical samples, and the cutoff points of the R value of A and B antigens were 1.483 and 1.576, respectively. Conclusion In this study, we reported a novel quantitative and multiplexed method for the identification of ABO blood groups and presented an effective alternative for quantitative blood typing. This method can be used as an effective tool to improve blood typing and further guarantee clinical transfusion safety.

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confidence: 99%

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Zhang

Fan

et al. 2017

IJN

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“…The normalized spectrum at t = 0 has a peak at 575 nm, which is in accord with previous reports. [12c] After 15 min of photobleaching at 488 nm, the autofluorescence reached a minimum value. The drop in the normalized value was large for shorter wavelengths (e.g., 4% remaining from the initial value at 520 nm) and small for longer wavelengths (e.g., 15% remaining from the initial value at 679 nm).…”

Section: Resultsmentioning

confidence: 99%

“…Relative to the emission filter bandwidth, the autofluorescence normalized spectral profile, S (λ), which fulfills the condition

\int S false(λ false) normald λ = 1

, has a wide bandwidth. [12c] Therefore, the fraction of emitted photons that passes the emission filter can be approximated by

\int [S (λ) \cdot F (λ)] \cdot normald λ ≅ S (λ_{em}) \cdot normalΔ λ_{normalem}

…”

Section: Methodsmentioning

confidence: 99%

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Improving the Sensitivity of Fluorescence‐Based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads

Roth

Hadass²,

Cohen

et al. 2018

Small

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In fluorescence‐based assays, usually a target molecule is captured using a probe conjugated to a capture surface, and then detected using a second fluorescently labeled probe. One of the most common capture surfaces is a magnetic bead. However, magnetic beads exhibit strong autofluorescence, which often overlaps with the emission of the reporter fluorescent dyes and limits the analytical performance of the assay. Here, several widely used magnetic beads are photobleached and their autofluorescence is reduced to 1% of the initial value. Their autofluorescence properties, including their photobleaching decay rates and autofluorescence spectra pre‐ and post‐photobleaching, and the stability of the photobleaching over a period of two months are analyzed. The photobleached beads are stable over time and their surface functionality is retained. In a high‐sensitivity LX‐200 system using photobleached magnetic beads, human interleukin‐8 is detected with a threefold improvement in detection limit and signal‐to‐noise ratio over results achievable with nonbleached beads. Since many contemporary immunoassays rely on magnetic beads as capture surfaces, prebleaching the beads may significantly improve the analytical performance of these assays. Moreover, nonmagnetic beads with low autofluorescence are also successfully photobleached, suggesting that photobleaching can be applied to various capture surfaces used in fluorescence‐based assays.

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“…Multicolor quantum dots enable visualization of various biomolecules over a wide range of emission wavelengths and are capable of optically detecting many biomarkers simultaneously. [11][12][13] Here we present an assay for prostate cancer diagnosis using magnetic microparticles (MMPs) for efficient capture of TMPRSS2 and oligonucleotide-functionalized multicolor quantum dots (QDs) for optical coding of rearranged TMPRSS2-ERG fusion genes ( Figure 1B, C and D). Our assay enables relatively fast and accurate detection of prostate cancer-specific fusion genes at a low concentration in buffer, urine, and prostate cancer cells (LNCaP and NCI-H660 cell lines).…”

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confidence: 99%

Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis

Lee¹,

Kim²,

Park³

et al. 2017

IJN

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Recent studies have found that prostate cancer expresses abnormal genetic markers including multiple types of TMPRSS2–ERG fusion genes. The expression level of different TMPRSS2–ERG fusion genes is correlated to pathologic variables of aggressive prostate cancer and disease progression. State-of-the-art methods for detection of TMPRSS2–ERG fusion genes include reverse transcription polymerase chain reaction (RT-PCR) with a detection limit of 1 fmol at urinary condition. RT-PCR is time consuming, costly, and inapplicable for multiplexing. Ability to identify multiple fusion genes in a single sample has become important for diagnostic and clinical purposes. There is a need for a sensitive diagnostic test to detect multiple TMPRSS2–ERG fusion genes for an early diagnosis and prognosis of prostate cancer. Here, we propose to develop an assay for prostate cancer diagnosis using oligonucleotide-functionalized quantum dot and magnetic microparticle for optical detection of rearranged TMPRSS2–ERG fusion genes at a low concentration in urine. We found that our assay was able to identify three different types of fusion gene with a wide detection range and detection limit of 1 fmol (almost the same level of the RT-PCR result reported). Here, we show detection of multiple TMPRSS2–ERG fusion genes using color-coded oligonucleotides in cell lysate and urine.

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Magnetic bead-quantum dot assay for detection of a biomarker for traumatic brain injury

Cited by 37 publications

References 41 publications

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Improving the Sensitivity of Fluorescence‐Based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads

Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis

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Magnetic bead-quantum dot assay for detection of a biomarker for traumatic brain injury

Cited by 37 publications

References 41 publications

Quantitative and multiplexed detection for blood typing based on quantum dot&ndash;magnetic bead assay

Quantitative and multiplexed detection for blood typing based on quantum dot&ndash;magnetic bead assay

Improving the Sensitivity of Fluorescence‐Based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads

Optical coding of fusion genes using multicolor quantum dots for prostate cancer diagnosis

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Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay

Quantitative and multiplexed detection for blood typing based on quantum dot–magnetic bead assay