Magnetic-bead-based microfluidic system for ribonucleic acid extraction and reverse transcription processes

Liu, Chien Ju; Lien, Kang Yi; Weng, Ching Yi; Shin, Jyh Wei; Chang, Tsuey Yu; Lee, Gwo‐Bin

doi:10.1007/s10544-008-9240-1

Cited by 44 publications

(32 citation statements)

References 33 publications

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“…Magnetic bead-based binding of aptamers and nucleic acid molecules to their molecular targets with high affinity and specificity has been reported by Lou et al (203). Lee and co-workers have demonstrated magnetic bead-based extraction of RNA (204). Streptavidin coated magnetic particles as solid supports have been reported by Peyman et al for bioanalysis in continuous flow (205).…”

Section: Standard Operationsmentioning

confidence: 99%

Latest Developments in Micro Total Analysis Systems

et al. 2010

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The field of micro total analysis systems (µTAS) (1) or lab on a chip (LOC) has extended its usefulness into many new fields and disciplines spanning basic research to commercial applications. The importance of µTAS is reflected in both the growing number and improved quality of published articles. µTAS has expanded into an incredibly diverse number of analytical chemistry applications and has received a great amount of input from a wide spectrum of scientific and engineering disciplines. In this sense, µTAS is highly interdisciplinary and has served as a focal point to bring together multidisciplinary research fields.This work builds on former reviews (2-6) and attempts to chart the most recent developments in µTAS. By cross referencing online keyword searches, several thousand articles were found spread among a wide variety of journals but were most frequently found in the high impact journals such as Science, Nature, PNAS, Analytical Chemistry, Lab on a Chip, and others. The annual international µTAS conference also provided articles on some of the very latest and most promising developments. This review article aims to report the latest achievements in the field of µTAS by highlighting some of the very best research papers published over the 2 year period between March 2008 and February 2010. Our main goal is to give a broad overview of the latest state-ofthe-art research and to introduce newcomers to the field. We were overwhelmed by the large number of research papers published on cellular analysis, manipulation, and droplet fluidics; therefore, we divided the review into two parts. This first part concentrates on reports related to manufacturing, technology, and instrumentation for molecular analysis, and part two covers applications related to cellular analysis and manipulation, which is referred to as cellson-a-chip (460). The papers we selected on droplet fluidics are distributed throughout both parts of the article according to their applications. We attempted to select papers based on the merit of their contents, which was a difficult task, and we have missed some good articles; for these oversights, we offer our apology in advance.Since this review focuses on novel developments of microfluidic systems for applied analytical chemical applications, publications about sensor arrays (so-called "biochips"), chemical synthesis on microchips (except some papers on particle synthesis using droplets), theory, and simulations, as well as trend articles, have been omitted. Furthermore, we reduced the technology section, as the majority of work is carried out by employing simple planar microchips fabricated in glass or polymers such as silicone elastomers. TECHNOLOGYMicrofabrication. Conventional Microfabrication. Many new innovative approaches have been reported in the field of microfabrication. Semicircular cross section channels network microfabricated on a silicon chip by XeF 2 etching by Camp et al. (7). These channels allow stress free transportation of cells. Generally, vapor deposited thin film metal ele...

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Section: Standard Operationsmentioning

confidence: 99%

Latest Developments in Micro Total Analysis Systems

et al. 2010

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“…Once attached, a wash solution removed any unbound molecules and the particles were collected. Lien et al used a meander-MET array beneath a microfluidic reaction chamber to trap and purify RNA-coated MPs [49,50]. Once purified, RNA was released from the MPs and the MET was used to heat the solution to a constant temperature to enable a reversetranscription process to occur.…”

Section: Mets In Microfluidicsmentioning

confidence: 99%

Applications of microelectromagnetic traps

Basore

Baker

2012

Anal Bioanal Chem

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Microelectromagnetic traps (METs) have been used for almost two decades to manipulate magnetic fields. Different trap geometries have been shown to produce distinct magnetic fields and field gradients. Initially, microelectromagnetic traps were used mainly to separate and concentrate magnetic material at small scales. Recently such traps have been implemented for unique applications, for example filterless bioseparations, inductive heat generation, and biological detection. In this review, we describe recent reports in which MET geometry, current density, or external fields have been used. Descriptions of recent applications in which METs have been used to develop sensors, manipulate DNA, or block ion current are also provided.

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“…Magnetic beads can also be manipulated by using electromagnets or permanent magnets. Therefore, collecting [9][10][11][12] or transporting [16][17][18][19][20][21][22][23] biological molecules using magnetic beads has been successfully demonstrated.…”

Section: Introductionmentioning

confidence: 99%

“…Biochip systems for different applications were developed using pneumatic actuation for controlling microvalves and micropumps [9][10][11] . The pneumatic actuation is robust and effective, while pneumatic links to external pneumatic sources are required.…”

Section: Introductionmentioning

confidence: 99%

An electromagnetically-driven microfluidic platform with indirect-heating thermo-pneumatic valves

et al. 2011

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In this work, we present a microfluidic platform which is capable of carrying out a series of laboratorial operations on a disposable chip. The platform employs coil arrays to transport biological samples attached on magnetic beads through different reaction zones in aqueous solutions. Micromachined thermopneumatic valves are adopted as the interfaces between sequential reactions. The self-contained system is composed of a disposable microfluidic reaction (MFR) chip and a fluidic driving/sensing (FDS) module. Various reaction zones with different functionalities are implemented on the MFR chip. Arrays of coils and micromachined heater/sensing chips are integrated into the FDS module. Also, novel indirect-heating thermo-pneumatic valves (IH-TPVs), which are monolithically integrated into the disposable MFR chip, are proposed and characterized. With an applied voltage of 3.2 V, the IH-TPV effectively isolates two adjacent reaction zones, while its temperature elevation is sufficiently low for biological samples and reagents. By employing the proposed approach, we design and implement a miniaturized biochip system which carries out the processes of DNA extraction, purification and amplification from whole blood sample. The dimension of the fully-integrated system is 72 mm×54 mm×11 mm, and the system could be fully operated with a 12 V DC power supply. DNA extraction and purification from human whole blood followed by PCR amplification for the 122 bp segment is successfully performed by using the system.

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Magnetic-bead-based microfluidic system for ribonucleic acid extraction and reverse transcription processes

Cited by 44 publications

References 33 publications

Latest Developments in Micro Total Analysis Systems

Latest Developments in Micro Total Analysis Systems

Applications of microelectromagnetic traps

An electromagnetically-driven microfluidic platform with indirect-heating thermo-pneumatic valves

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