Magnetic bead-based immunoassays for the quantification of human and mouse cytokines (65.31)

Abstract

Multiplexing immunoassays have become a very powerful and economic way to measure multiple analytes from a single sample. This is especially important when sample volumes are limited, such as for rodent samples. Life Technologies has recently launched a set of luminex bead-based immunoassays that can conveniently quantify between 10 and 30 cytokines from human or mouse tissue culture, serum and plasma samples. These assays use magnetic beads which offer a convenient way to automate these assays using commercially available plate washers, such as BioTek ELx405 or Tecan HydroSpeed. The mouse analytes in these kits are: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 p40/p70, TNF-α (10-plex), supplemented with FGF basic, IL-1α, IL-13, IL-17, IP-10, KC, MCP-1, MIG, MIP-1α and VEGF (mouse 20-plex). Human analytes are GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 and TNF-α (10-plex), supplemented with IL-1RA, IL-2R, IL-7, IL-12 p40/p70, IL-13, IL-15, IL-17, IFN-α, MIP-1α, MIP-1β, IP-10, MIG, eotaxin, RANTES, MCP-1, VEGF, G-CSF, FGF basic and HGF (human 30-plex). All the kits have been extensively validated to include sample recoveries routinely between 80 and 120%, intra-assay and inter-assay precision of <10%, and linearity of dilution with tissue culture medium and serum with R2 values of 0.98 and greater. Benchmarking of the kits has been performed against our ELISA kits to ensure equal results between the two immunoassay platforms.