Magnetic bead based immuno-detection of Listeria monocytogenes and Listeria ivanovii from infant formula and leafy green vegetables using the Bio-Plex suspension array system

Day, James B.; Basavanna, Uma

doi:10.1016/j.fm.2014.09.020

Cited by 17 publications

(7 citation statements)

References 53 publications

(49 reference statements)

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“…; De Lappe et al . ; Day and Basavanna ). Molecular techniques, such as real‐time PCR, have been employed to detect L. monocytogenes in various fruits including apples, grapes, cantaloupe, mango, strawberries and oranges which achieve high sensitivities but require multiple days of enrichment (Shearer et al .…”

Section: Discussionmentioning

confidence: 96%

“…Previous immunological techniques used for L. monocytogenes detection in foods (ELISA, fibre-optic immune-sensors, magnetic bead-antibody conjugates) have been limited due to low sensitivity, misidentifications, prolonged detection times or susceptibility to false-positive results (Geng et al 2004;Johnson et al 2013;De Lappe et al 2014;Day and Basavanna 2015). Molecular techniques, such as real-time PCR, have been employed to detect L. monocytogenes in various fruits including apples, grapes, cantaloupe, mango, strawberries and oranges which achieve high sensitivities but require multiple days of enrichment (Shearer et al 2001;Badosa et al 2009).…”

Section: Discussionmentioning

confidence: 99%

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Immuno‐detection and differentiation ofListeria monocytogenesandListeria ivanoviiin stone fruits

Day

Hammack

2019

J Appl Microbiol

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Aims The aim of this study was to develop a rapid detection and differentiation method for pathogenic Listeria species in stone fruits. Methods and Results We utilized activated charcoal enrichment media (ACM) to induce overexpression and hypersecretion of pathogenic Listeria virulence proteins which can subsequently be detected via immunoblot analysis. Plum and nectarine slices spiked with either L. monocytogenes or L. ivanovii were incubated in pre‐enrichment broth followed by enrichment in ACM. Secreted proteins were precipitated and subjected to SDS‐PAGE and immunoblot analysis using a combination of L. monocytogenes‐specific antibody (α‐listeriolysin O) and antibody specific for both L. monocytogenes and L. ivanovii (α‐Internalin C). As low as 1 CFU per gram of L. monocytogenes in plum and nectarine was detected, whereas a detection limit of 10 CFU per gram was achieved for L. ivanovii in each food tested following a 20‐h enrichment period. Nonpathogenic Listeria species and non‐Listeria bacterial pathogens tested were negative. Conclusions These results demonstrate the highly sensitive and specific nature of the detection method for pathogenic Listeria in stone fruits using activated charcoal enrichment as well as the capability to discriminate between L. monocytogenes and L. ivanovii. Significance and Impact of the Study This method is the first to identify and differentiate L. monocytogenes and L. ivanovii in select stone fruit enrichments within 24 h using immunological techniques. The rapidity and sensitivity of the method could aid in the reduction of exposure to the public in the event of an outbreak and expedite the administration of appropriate antibiotics to infected individuals.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Immuno‐detection and differentiation ofListeria monocytogenesandListeria ivanoviiin stone fruits

Day

Hammack

2019

J Appl Microbiol

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“…The outer polymeric membrane serves as a matrix in which the surface functional groups are added, and also protects the core of the metal from the external environment. The outer shell may also consist of agarose, cellulose, porous glass or silicon dioxide (Day et al, 2015).…”

Section: Characteristics Of Magnetic Particles and Methods For Obtainmentioning

confidence: 99%

“…The use of magnetic nanotechnologies in laboratory diagnostics allows the elimination of the imperfections and limitations that are typical of traditional immunoassays (enzyme-linked immunosorbent assay, immunofluorescence, etc.) (Mani et al, 2011;Day et al, 2015;Wang et al, 2017a;Liao et al, 2017). In particular, it becomes possible to disclaim strict requirements to the "purity" of the tested material, but also expand the range of materials that can be tested; it is possible to increase the analytical sensitivity of the analysis, to improve other bioanalytical characteristics, etc.…”

Section: Introductionmentioning

confidence: 99%

Modern magnetic immunoassay: Biophysical and biochemical aspects

Galkin

Besarab

Pysmenna

et al. 2017

Regul. Mech. Biosyst.

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In this review article an analysis of the biochemical and biophysical aspects of modern magnetic immunoassay (MIA) is conducted and additionally the problems and perspectives of its application in biology, biotechnology and medicine are defined. Magnetic immunoassay should be considered as an evolutionary extension of the classical immunoassay. MIA can have many variants of modifications, similar to the classic immunoenzymatic assay. The key distinctive element of the MIA is the use of magnetic particles (MPs), which are usually nanoparticles. MPs in the MIA can act as a marker for detection, or the solid phase at which the immunochemical reaction takes place. MIA possesses basic advantages over classical immunoassay methods: thanks to the unique magnetic properties of the MPs and the ability to manipulate it in the external magnetic field, it is possible to increase the informative value of the analysis (first of all, sensitivity and specificity), as well as the rigid requirements for “purity” of tested samples. For the purposes of immunoassay, magnetic particles of size from 10 to 200 nm are important, since such particles are in a superparamagnetic state, in the absence of strong magnetic fields; they are not agglomerated in a liquid medium. The size of the spherical particle determines the rate of sedimentation and mobility in the solution. The outer polymeric membrane serves as a matrix in which the surface functional groups are added, and also protects the core of the metal from the external environment. The outer shell may also consist of agarose, cellulose, porous glass, silicon dioxide etc. There are several strategies for the synthesis of nanoparticles: mechanical (dispersion), physical (gas phase deposition), wet chemical methods (chemical comprecipitation, thermal decomposition, methods of micro emulsion, hydrothermal reactions) and physico-chemical methods. Also used are magnesite nanoparticles of biogenic origin. Magnetic particles may function, and this is important for immunoassay. Surface functional groups include carboxylic, amino, epoxy, hydroxyl, tosyl, and N-hydroxysuccinate-activated groups. Magnetic spherical particles usually interact with surface molecules such as streptovidine, biotin, protein A, protein G, and immunoglobulin etc. Directions and prospects of the development of methods of magnetic immunoassay are determined, mainly, by the development of methods for detecting or influencing magnetic particles. In this case, the classical methods of detection are electrochemical methods, electrochemiluminescence, fluorescence. More modern ones include giant magnetoresistance, superconducting quantum interference devices, surface-enhanced Raman spectroscopy, biosensors based on nonlinear magnetization, magneto-PCR immunoassay. The current trend is to combine or integrate the application of various biochemical, physical, molecular and genetic, physico-chemical detection methods. In fact, all of these benefits undoubtedly open up broad prospects for the practical application of MIA in biology, biotechnology and medicine.

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“…However, these previous studies did not validate their systems using enriched food samples. Another group used the suspension bead array to detect contaminations of L. monocytogenes and L. ivanovii in baby milk powder and vegetables using macrophage enrichment (Day and Basavanna, 2015). However, no one has ever reported the use of this immunobead array method with enriched food samples for detection of L. monocytogenes, Salmonella spp.…”

Section: Introduction 48mentioning

confidence: 99%

Validation of a high-throughput immunobead array technique for multiplex detection of three foodborne pathogens in chicken products

Charlermroj

Makornwattana

Grant

et al. 2016

International Journal of Food Microbiology

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This study rigorously evaluated a previously developed immunobead array method to simultaneously detect three important foodborne pathogens, Campylobacter jejuni, Listeria monocytogenes, and Salmonella spp., for its actual application in routine food testing. Due to the limitation of the detection limit of the developed method, an enrichment step was included in this study by using Campylobacter Enrichment Broth for C. jejuni and Universal Pre-enrichment Broth for L. monocytogenes and Salmonella spp.. The findings showed that the immunobead array method was capable of detecting as low as 1CFU of the pathogens spiked in the culture media after being cultured for 24h for all three pathogens. The immunobead array method was further evaluated for its pathogen detection capabilities in ready-to-eat (RTE) and ready-to-cook (RTC) chicken samples and proven to be able to detect as low as 1CFU of the pathogens spiked in the food samples after being cultured for 24h in the case of Salmonella spp., and L. monocytogenes and 48 h in the case of C. jejuni. The method was subsequently validated with three types of chicken products (RTE, n=30; RTC, n=20; raw chicken, n=20) and was found to give the same results as the conventional plating method. Our findings demonstrated that the previously developed immunobead array method could be used for actual food testing with minimal enrichment period of only 52 h, whereas the conventional ISO protocols for the same pathogens take 90-144 h. The immunobead array was therefore an inexpensive, rapid and simple method for the food testing.

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Magnetic bead based immuno-detection of Listeria monocytogenes and Listeria ivanovii from infant formula and leafy green vegetables using the Bio-Plex suspension array system

Cited by 17 publications

References 53 publications

Immuno‐detection and differentiation ofListeria monocytogenesandListeria ivanoviiin stone fruits

Immuno‐detection and differentiation ofListeria monocytogenesandListeria ivanoviiin stone fruits

Modern magnetic immunoassay: Biophysical and biochemical aspects

Validation of a high-throughput immunobead array technique for multiplex detection of three foodborne pathogens in chicken products

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