Magnetic bead-based electrochemical and colorimetric assays of circulating tumor cells with boronic acid derivatives as the recognition elements and signal probes

Xia, Ning; Wu, Daohong; Yu, Hongfang; Sun, Wanwan; Yi, Xinyao; Liu, Lin

doi:10.1016/j.talanta.2020.121640

Cited by 31 publications

(22 citation statements)

References 64 publications

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“…decrease in the electrochemical signal of FcBA or preventing the MPBA-triggered aggregation of AuNPs, which leads to changes in electrochemical signal or color. The colorimetric assays achieved a linear range of 50-1.5 × 10 4 cells mL −1 with a sensitivity of 50 cells mL −1 [143].…”

Section: Poct Based Detection Of Enzyme Activity/proteins/compoundmentioning

confidence: 98%

“…CTCs were firstly captured by aptamer-modified magnetic beads, and then they can sequestrate ferroceneboronic acid (FcBA) or 4-mercaptophenylboronic acid (MPBA) by the formation of boronate ester bonds, thus leading to the decrease in the electrochemical signal of FcBA or preventing the MPBA-triggered aggregation of AuNPs, which leads to changes in electrochemical signal or color. The colorimetric assays achieved a linear range of 50-1.5 × 10 4 cells mL −1 with a sensitivity of 50 cells mL −1 [143].…”

Section: Poct Based Detection Of Ctcmentioning

confidence: 98%

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Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Han

Sun

et al. 2020

Sensors

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Circulating tumor cells (CTCs) are tumor cells that escaped from the primary tumor or the metastasis into the blood and they play a major role in the initiation of metastasis and tumor recurrence. Thus, it is widely accepted that CTC is the main target of liquid biopsy. In the past few decades, the separation of CTC based on the electrochemical method has attracted widespread attention due to its convenience, rapidness, low cost, high sensitivity, and no need for complex instruments and equipment. At present, CTC detection is not widely used in the clinic due to various reasons. Point-of-care CTC detection provides us with a possibility, which is sensitive, fast, cheap, and easy to operate. More importantly, the testing instrument is small and portable, and the testing does not require specialized laboratories and specialized clinical examiners. In this review, we summarized the latest developments in the electrochemical-based CTC detection and point-of-care CTC detection, and discussed the challenges and possible trends.

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Section: Poct Based Detection Of Enzyme Activity/proteins/compoundmentioning

confidence: 98%

Section: Poct Based Detection Of Ctcmentioning

confidence: 98%

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Han

Sun

et al. 2020

Sensors

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“…Phenylboronic acid (PBA) and its derivates can bind with α-hydroxycarboxylate acids (such as citrate and tartrate) and o-diphenol/diol-containing species (such as catechol derivatives, nucleosides, and glycoproteins) via the formation of a covalent boronate ester bond [61]. Such interactions have been introduced into the design of various biosensors [62][63][64]. Capping reagents play an important role in the enhancement of the stability and solubility of nanomaterials [65,66].…”

Section: Metal-thiol and Boronate Ester Interactionsmentioning

confidence: 99%

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

et al. 2021

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The physiochemical properties of nanomaterials have a close relationship with their status in solution. As a result of its better simplicity than that of pre-assembled aggregates, the in situ assembly of nanomaterials has been integrated into the design of electrochemical biosensors for the signal output and amplification. In this review, we highlight the significant progress in the in situ assembly of nanomaterials as the nanolabels for enhancing the performances of electrochemical biosensors. The works are discussed based on the difference in the interactions for the assembly of nanomaterials, including DNA hybridization, metal ion–ligand coordination, metal–thiol and boronate ester interactions, aptamer–target binding, electrostatic attraction, and streptavidin (SA)–biotin conjugate. We further expand the range of the assembly units from nanomaterials to small organic molecules and biomolecules, which endow the signal-amplified strategies with more potential applications.

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“…In the physiological pH range, boronic acids of 4-MPBA can stably combine with cis -diols of bacteria . Notably, 4-MPBA is not specific for different bacterial recognition, so aptamers with specific recognition ability to bacteria can be used in conjunction with 4-MPBA …”

Section: Introductionmentioning

confidence: 99%

Zirconium-Based Metal–Organic Framework and Ti₃C₂T_x Nanosheet-Based Faraday Cage-Type Electrochemical Aptasensor for Escherichia coli Detection

Dai

et al. 2022

ACS Appl. Nano Mater.

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A Faraday-cage-type electrochemical aptasensor based on two-dimensional (2D) nanomaterials was developed for the sensitive detection of Escherichia coli O157:H7. In this electrochemical aptasensor, 2D titanium carbide (Ti3C2T x ) MXene was used for electrode modification. 2D Ti3C2T x could immobilize aptamers via chelation between titanium and phosphate groups and provide a large electroactive surface for signal transduction. Another 2D zirconium ferrocene-based metal–organic framework (Zr-Fc MOF) combined with gold nanoparticles (AuNPs) and 4-mercaptophenylboronic acid (4-MPBA) (Zr-Fc MOF/AuNPs/4-MPBA) was used as an electrochemical signal label, in which Fc was a signal molecule and AuNPs could improve the electroactivity and combine with 4-MPBA via Au–S bonds. 4-MPBA could bind with E. coli O157:H7 via covalent bonding between boronic acid and the cis-diol of lipopolysaccharides on bacteria cell walls. So, the signal labels could immobilize on the electrode to form a Faraday-cage-type structure owing to the large surface area of Zr-Fc MOF. In this structure, electrons flowed directly between the electrode and ferrocene, and the electrochemical signal could be amplified. When the application of 2D nanomaterials and the Faraday-cage strategy were combined, E. coli O157:H7 was sensitively detected with a detection limit of 3 CFU·mL–1. The aptasensor was applied for milk sample detection. This aptasensor has practical application potential because of its properties of satisfactory sensitivity, specificity, and stability.

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Magnetic bead-based electrochemical and colorimetric assays of circulating tumor cells with boronic acid derivatives as the recognition elements and signal probes

Cited by 31 publications

References 64 publications

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

Zirconium-Based Metal–Organic Framework and Ti₃C₂T_x Nanosheet-Based Faraday Cage-Type Electrochemical Aptasensor for Escherichia coli Detection

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Magnetic bead-based electrochemical and colorimetric assays of circulating tumor cells with boronic acid derivatives as the recognition elements and signal probes

Cited by 31 publications

References 64 publications

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

Electrochemical Detection and Point-of-Care Testing for Circulating Tumor Cells: Current Techniques and Future Potentials

In Situ Assembly of Nanomaterials and Molecules for the Signal Enhancement of Electrochemical Biosensors

Zirconium-Based Metal–Organic Framework and Ti3C2Tx Nanosheet-Based Faraday Cage-Type Electrochemical Aptasensor for Escherichia coli Detection

Contact Info

Product

Resources

About

Zirconium-Based Metal–Organic Framework and Ti₃C₂T_x Nanosheet-Based Faraday Cage-Type Electrochemical Aptasensor for Escherichia coli Detection