Magnetic bead and gold nanoparticle probes based immunoassay for β-casein detection in bovine milk samples

Li, Y.S.; Meng, Xuanyu; Zhou, Yu; Zhang, Y.Y.; Meng, Xian-Mei; Li, Yang; Hu, Pan; Lu, Shiying; Ren, Hong-Lin; Liu, Z.S.; Wang, X.R.

doi:10.1016/j.bios.2014.12.025

Cited by 29 publications

(5 citation statements)

References 33 publications

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“…Magnetic bead based on immunoassay was first established in the 1980s because of the main advantages of increasing the surface area for immobilization of antibody or antigen, reducing the incubation time from hours to minutes, and improving sensitivity of the assay (Biagini et al, 2004;Moscoso, Kiefer, Kutlar, & Garver, 1988). Magnetic bead-based immunoassay was comprehensively applied in detection of macromolecular substances, such as β-bungarotoxin (Mu et al, 2014), β-casein allergen (Li et al, 2015;Song et al, 2014), enterobacter cloacae (Zhang, Zhou, Zhang, Zhang, & Su, 2016), pathogens, and cancer biomarkers (Baniukevic et al, 2013;Chen et al, 2016;Shukla, Lee, Song, Park, & Kim, 2016). However, the application of goldmag-based immunoassay in the quantification of α-LA content in milk has not been studied.…”

Section: Introductionmentioning

confidence: 99%

Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk

Chao

et al. 2017

Food and Agricultural Immunology

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In this work, a goldmag-based enzyme-linked immunosorbent assay was developed for determination of α-lactalbumin (α-LA) in milk. The magnetic nanoparticle functionalized with polyetherimide was synthesized by one-pot method and coated with two layers of gold nanoparticles on the surface to synthesize goldmag nanoparticles. Anti-α-LA monoclonal antibody, prepared by hybridoma cell lines via cell fusion, was then bound to this goldmag nanoparticle to develop a capture nanoprobe. The results showed that this developed immunoassay had a good linear range of 2.33-127.1 ng/mL with IC 50 of 17.2 ng/mL. Besides, recovery rates for α-LA in four commercial milks were from 86.7% to 109.8%. The coefficients of variation in intra-assay and interassay were 3.9-6.8% and 5.5-9.8%, separately, which could meet the requirements to quantify α-LA content in milk. This goldmagbased immunoassay might have considerable potentials in the detection of food allergies. ARTICLE HISTORY

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Section: Introductionmentioning

confidence: 99%

Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk

Chao

et al. 2017

Food and Agricultural Immunology

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“…Hence, non-enzymatic labels for detection are being and have been developed, such as beads [ 19 ] and metal nanoparticles [ 16 , 17 , 20 , 21 , 22 , 23 , 24 , 25 ]. Ye et al used gold vesicles encapsulated with Pd-Ir nanoparticles (Pd-Ir NPs@GVs) as peroxidase mimics by raising the temperature.…”

Section: Introductionmentioning

confidence: 99%

Anthracycline-Functionalized Dextran as a New Signal Multiplication Tagging Approach for Immunoassay

et al. 2023

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The most used kind of immunoassay is enzyme-linked immunosorbent assay (ELISA); however, enzymes suffer from steric effects, low stability, and high cost. Our research group has been developing quinone-linked immunosorbent assay (QuLISA) as a new promising approach for stable and cost-efficient immunoassay. However, the developed QuLISA suffered from low water-solubility of synthesized quinone labels and their moderate sensitivity. Herein, we developed a new approach for signal multiplication of QuLISA utilizing the water-soluble quinone anthracycline, doxorubicin, coupled with dextran for signal multiplication. A new compound, Biotin-DexDox, was prepared in which doxorubicin was assembled on oxidized dextran 40, and then it was biotinylated. The redox-cycle-based chemiluminescence and the colorimetric reaction of Biotin-DexDox were optimized and evaluated, and they showed very good sensitivity down to 0.25 and 0.23 nM, respectively. Then, Biotin-DexDox was employed for the detection of biotinylated antibodies utilizing avidin as a binder and a colorimetric assay of the formed complex through its contained doxorubicin redox reaction with NaBH4 and imidazolium salt yielding strong absorbance at 510 nm. The method could detect the plate-fixed antibody down to 0.55 nM. Hence, the application of Biotin-DexDox in QuLISA was successfully demonstrated and showed a significant improvement in its sensitivity and applicability to aqueous assays.

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“…18 In the meantime, some novel b-LG detection technologies are emerging, such as biosensor technology 19 and magnetic nanomicrosphere technology. 20 However, existing detection methods have distinct disadvantages, such as time consumption, dependence on skilled operators, and expensive equipment. Therefore, new biosensors are urgently needed to achieve rapid and low-cost detection and analysis of b-LG.…”

Section: Introductionmentioning

confidence: 99%

A fluorescence detection method for the determination of β-lactoglobulin in foods

2022

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Magnetic bead and gold nanoparticle probes based immunoassay for β-casein detection in bovine milk samples

Cited by 29 publications

References 33 publications

Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk

Goldmag-based enzyme-linked immunosorbent assay for determination of α-lactalbumin in milk

Anthracycline-Functionalized Dextran as a New Signal Multiplication Tagging Approach for Immunoassay

A fluorescence detection method for the determination of β-lactoglobulin in foods

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