Magnetic-activated cell sorting is not completely effective at reducing sperm DNA fragmentation

Martínez, Mercedes González; Sánchez-Martín, Pascual; Dorado-Silva, Mónica; Fernández, José Luis Fernández; Girones, Estibaliz; Johnston, Stephen D.; Gosálvez, Jaime

doi:10.1007/s10815-018-1319-x

Cited by 18 publications

(13 citation statements)

References 30 publications

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“…Live birth rates were reported ( Gil et al ., 2013 ). The ability of MACS to effectively select sperm with reduced DNA fragmentation has been recently questioned ( Martínez et al ., 2018 ), and although its use did not improve live birth rates, fewer miscarriages were recorded ( Sánchez-Martín et al ., 2017 ).…”

Section: Resultsmentioning

confidence: 99%

Sperm selection in IVF: the long and winding road from bench to bedside

Pedrosa¹,

Furtado

Ferreira³

et al. 2020

JBRA Assisted Reproduction

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Spermatozoa wage battle to conquer fertilization but the traits needed to succeed remain elusive. The natural advantageous qualities that enable only a few selected sperm cells to reach the site of fertilization remain unknown. Although in vitro fertilization (IVF) facilitates the job of spermatozoa, a universally acceptable means of sperm selection is yet to be developed. No objective or reliable sperm quality indicators have been established and sperm selection is, to a great extent, based on subjective qualitative evaluation. The best method for sperm selection in IVF presents several challenges: intrinsic sperm qualities cannot be evaluated and the ideal endpoint for these studies is debatable. An ideal method for sperm selection in ART should be noninvasive and cost-effective, and allow the identification of high-quality spermatozoa and yield better outcomes in terms of pregnancy and live birth rates. This narrative review included 85 papers and focused on the new available methods and technologies that might shed some light on sperm selection in IVF. It discusses the available data on microfluidic devices, omics profiling, micronuclei studies, sperm plasma membrane markers, and other techniques, such as Magnetic Activated Cell Sorting (MACS), Raman micro-spectroscopy, and artificial intelligence systems. The new techniques herein reviewed offer fresh approaches to an old problem, for which a definite solution has yet to cross the bridge from bench to IVF clinics around the world, since clinical usefulness and application remain unproven.

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Section: Resultsmentioning

confidence: 99%

Sperm selection in IVF: the long and winding road from bench to bedside

Pedrosa¹,

Furtado

Ferreira³

et al. 2020

JBRA Assisted Reproduction

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“…In a sub‐analysis, the in‐house designed swim‐out methods were compared to the commercial Magnetic‐activated cell sorting test (MACS ® ART Annexin V, Miltenyi Biotec, 2016). Several studies have shown the potential of MACS selected spermatozoa for fertility treatment (Martínez et al., 2018; Sánchez‐Martín et al., 2017). These studies assumed an association of sperm apoptosis with sperm DNA damage (Martínez et al., 2018; Sánchez‐Martín et al., 2017), and therefore, SDF is often used as outcome parameter in studies comparing semen preparation methods with MACS ® .…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have shown the potential of MACS selected spermatozoa for fertility treatment (Martínez et al., 2018; Sánchez‐Martín et al., 2017). These studies assumed an association of sperm apoptosis with sperm DNA damage (Martínez et al., 2018; Sánchez‐Martín et al., 2017), and therefore, SDF is often used as outcome parameter in studies comparing semen preparation methods with MACS ® . The mean SDF rate was significantly reduced after density gradient and MACS ® (1.69 ± 3.57%) compared with density gradient prepared spermatozoa (2.46 ± 7.70%) or native spermatozoa (7.01 ± 4.31%) ( p ≤ .001).…”

Section: Discussionmentioning

confidence: 99%

A methodological validation of an easy one‐step swimout semen preparation procedure for selecting DNA fragmentation‐free spermatozoa for ICSI

et al. 2020

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The main purpose of this methodological paper was to describe a recently designed one‐step ICSI semen preparation swim‐out method (called swim‐ICSI) and to compare its efficacy with our conventional two‐step swim‐out method for the selection of motile spermatozoa for ICSI with minimal DNA damage. In this observational cohort study, 42 fresh ejaculate sperm samples for ICSI were included to compare the new swim‐ICSI with the conventional swim‐out. In a sub‐analysis (n = 20), both in‐house designed ICSI preparation methods were compared with a commercial magnetic‐activated cell sorting test (MACS®). Sperm DNA fragmentation (SDF), using Halosperm®, was determined at different time points during sperm preparation: on the native sample (a), after density gradient centrifugation (DG) (b), on the motile (A + B) spermatozoa selected with conventional swim‐out post‐DG (c) and selected with swim‐ICSI method post‐DG (d). For a subgroup (n = 20), SDF was also calculated after MACS (e). The mean SDF significantly reduced after EACH preparation step and reduced to almost zero in the recovered A + B spermatozoa when the semen prepared with DG was further processed for ICSI (swim‐ICSI vs. swim‐out, p = .001). In conclusion, the optimised one‐step and fine‐tuned swim‐ICSI technique shows the possibility to select a population of spermatozoa with almost zero SDF to be used in ICSI treatments.

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“…Magnetic-activated cell sorting (MACS) depends on selecting preferred sperm based on membrane surface markers, with the aim of excluding apoptotic and DNA damaged sperm. However, MACS is not completely effective at reducing sperm DNA fragmentation 84 and improvement in ART outcomes are variable. [85][86][87] Sperm with high negative surface electrical charge, named 'zeta potential', are mature and more likely to have intact chromatin.…”

Section: New Developments In Sperm Selection In Artmentioning

confidence: 99%

An update on the management of male infertility

Karavolos

Panagiotopoulou²,

AlAhwany

et al. 2020

The Obstetric & Gynaecologis

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Male infertility underlies or contributes to up to 50% of infertility cases; current therapeutic interventions rely on assisted reproductive technology (ART), as medical or surgical treatments have limited value in enhancing semen quality or parameters. Lifestyle factors that affect male fertility could offer a therapeutic opportunity; however, their modification seems to be of variable benefit. In the quest for sperm functional assessment and selection tests, there is controversy over which patients, if any, should be tested for sperm DNA fragmentation, as well as which test to perform. Sperm selection techniques for intracytoplasmic sperm injection do not appear to significantly improve treatment outcomes or live birth rates. Routinely performed genetic tests are effective in determining aetiology in approximately 20% of infertile men; however, newer genetic tests could enhance diagnosis and change the future management of male infertility. Learning objectives To summarise the key lifestyle factors that affect male fertility. To appraise the currently available investigations for sperm testing and selection. To describe the genetic tests currently available to identify the aetiology of male infertility, including emerging technologies in the field of genetics and personalised genomics. Ethical issues How to deal with couples' requests for unproven medical interventions to manage male infertility?

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Magnetic-activated cell sorting is not completely effective at reducing sperm DNA fragmentation

Cited by 18 publications

References 30 publications

Sperm selection in IVF: the long and winding road from bench to bedside

Sperm selection in IVF: the long and winding road from bench to bedside

A methodological validation of an easy one‐step swimout semen preparation procedure for selecting DNA fragmentation‐free spermatozoa for ICSI

An update on the management of male infertility

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