Magnet-actuated droplet microfluidic immunosensor coupled with gel imager for detection of microcystin-LR in aquatic products

Guan, Tian; Liu, Dayu; Li, Xiangmei; Shu, Bowen; Li, Mengting; Liu, Yingju; Xu, Zhen-Lin; Shen, Yu-Dong; Sun, Yuan; Lei, Hongtao; Shen, Xing

doi:10.1016/j.talanta.2020.121329

Cited by 15 publications

(5 citation statements)

References 34 publications

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“…Microchip capillary electrophoresis with laser-induced fluorescence (MCE–LIF) combines sample processing, injection, separation, and detection on a chip of several square centimeters as a separation and analysis technology via the integration of chemical synthesis, bioanalysis, optics, and information technology. − MCE–LIF has the advantages of reduced sample consumption, fast turnaround time, low cost provided by microfluidics, a multiplex capillary array to reduce the background interferents of separation, online detection, and high throughput analysis, , simple fluorescence labeling, high sensitivity, and various detection schemes . Sodium dodecyl sulfate was used as a dynamic coating modifier to separate fluorescein gizzerosine treated with fluorescein isothiocyanate on glass microchips via MCE followed by the rapid detection of the gizzerosine content in fish meal via LIF (Figure D).…”

Section: Detection Of Gizzerosinementioning

confidence: 99%

Formation and Detection of Gizzerosine in Animal Feed Matrices: Progress and Perspectives

Jiao,

Qian

et al. 2024

J. Agric. Food Chem.

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Gizzerosine is responsible for gizzard erosion and black vomit, owing to excessive gastric acid secretion in poultry. It is a biogenic amine that forms during feed processing. Gizzerosine, a derivative of histamine, is a serious threat to animal feed safety and poultry production because it is more potent after ingestion and more harmful to poultry than histamine. The difficulty of obtaining gizzerosine and the lack of simple, rapid, and sensitive in vitro detection techniques have hindered studies on the effects of gizzerosine on gizzard health and poultry production. In this review, we evaluated the natural formation and the chemical synthesis methods of gizzerosine and introduced seven detection methods and their principles for analyzing gizzerosine. This review summarizes the issues of gizzerosine research and suggests methods for the future development of gizzerosine detection methods.

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Section: Detection Of Gizzerosinementioning

confidence: 99%

Formation and Detection of Gizzerosine in Animal Feed Matrices: Progress and Perspectives

Jiao,

Qian

et al. 2024

J. Agric. Food Chem.

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“…To carry out the continuous pretreatment operation of biological detection reagents, a microfluidic bioseparation chip integrating mixer, heater, and soft magnet were proposed for nucleic acid detection and magnetic bead extraction of target substances [ 71 ]. Recently, Guan et al [ 72 ] successfully prepared a novel magnetically driven droplet microfluidic immunosensor without a complex operating system for the enrichment of cyanobacterial toxins in aquatic products. Compared with widely used microfluidic systems, the proposed sensor has two significant advantages: simple and effective operation by magnetic force alone rather than inconvenient actuation devices, and high-throughput parallel detection on a 15-channel chip.…”

Section: Application Of Microfluidic Chips In Sample Pretreatment Of Foodborne Pathogensmentioning

confidence: 99%

Recent advancements in microfluidic chip biosensor detection of foodborne pathogenic bacteria: a review

Wang

et al. 2022

Anal Bioanal Chem

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Foodborne diseases caused by pathogenic bacteria pose a serious threat to human health. Early and rapid detection of foodborne pathogens is an urgent task for preventing disease outbreaks. Microfluidic devices are simple, automatic, and portable miniaturized systems. Compared with traditional techniques, microfluidic devices have attracted much attention because of their high efficiency and convenience in the concentration and detection of foodborne pathogens. This article firstly reviews the bio-recognition elements integrated on microfluidic chips in recent years and the progress of microfluidic chip development for pathogen pretreatment. Furthermore, the research progress of microfluidic technology based on optical and electrochemical sensors for the detection of foodborne pathogenic bacteria is summarized and discussed. Finally, the future prospects for the application and challenges of microfluidic chips based on biosensors are presented. Graphical abstract

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“…Considering the long-term health and safety of humans, the World Health Organization stipulates that the maximum residue limit standard value of MC-LR in drinking water is 1.0 μg/L. 5 Therefore, monitoring of MC-LR is important to the development of society. Currently, available analytical methods for detecting microcystin mainly include high-performance liquid chromatography (HPLC), 6 liquid chromatography−tandem mass spectrometry, 7 immunoassay test, 8 and various other sensing methods.…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, some secondary metabolites will be released from the blue-green alga and cause serious illness. − Microcystin-LR (MC-LR), as an annular 7-peptide biotoxin, will cause acute poisoning of humans or animals, which seriously threatens the liver, kidney, stomach, immune and nervous systems, etc. Considering the long-term health and safety of humans, the World Health Organization stipulates that the maximum residue limit standard value of MC-LR in drinking water is 1.0 μg/L . Therefore, monitoring of MC-LR is important to the development of society.…”

Section: Introductionmentioning

confidence: 99%

Light-Initiated Imprinted Membrane-Based Biomimetic SERS Sensor toward Selective Detection of Trace MC-LR

Li,

Wang,

Zhang

et al. 2024

Anal. Chem.

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Microcystin-LR (MC-LR) is a severe threat to human and animal health; thus, monitoring it in the environment is essential, especially in water quality protections. Herein, in this work, we synthesize PVDF/CNT/Ag molecular imprinted membranes (PCA-MIMs) via an innovative combination of surface-enhanced Raman spectroscopy (SERS) detection, membrane separation, and molecular-imprinted technique toward the analysis of MC-LR in water. In particular, a light-initiated imprint is employed to protect the chemical structure of the MC-LR molecules. Furthermore, in order to ensure the detection sensitivity, the SERS substrates are combined with the membrane via the assistance of magnetism. The effect of synthesis conditions on the SERS sensitivity was investigated in detail. It is demonstrated from the characteristic results that the PCA-MIMs present high sensitivity to the MC-LR molecules with excellent selectivity against the interfere molecules. Results clearly show that the as-prepared PCA-MIMs hold great potential applications to detect trace MC-LR for the protection of water quality.

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Magnet-actuated droplet microfluidic immunosensor coupled with gel imager for detection of microcystin-LR in aquatic products

Cited by 15 publications

References 34 publications

Formation and Detection of Gizzerosine in Animal Feed Matrices: Progress and Perspectives

Formation and Detection of Gizzerosine in Animal Feed Matrices: Progress and Perspectives

Recent advancements in microfluidic chip biosensor detection of foodborne pathogenic bacteria: a review

Light-Initiated Imprinted Membrane-Based Biomimetic SERS Sensor toward Selective Detection of Trace MC-LR

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