Magnesium doping on TiN coatings affects mesenchymal stem cell differentiation and proliferation positively in a dose-dependent manner

Onder, Sakip; Calikoglu-Koyuncu, Ayse Ceren; Kazmanlı, Kürşat; Ürgen, Mustafa; Kök, Fatma Neşe; Torun-Kose, Gamze

doi:10.3233/bme-181000

Cited by 17 publications

(19 citation statements)

References 23 publications

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“…Similar positive effects of gelatin on the adhesion and proliferation of cells were also observed in another study (Yue et al, 2015). It has been reported that the excessive Mg 2+ might be cytotoxic to biological cells (Mangalampalli et al, 2018;Onder et al, 2018). The initial burst release of Mg 2+ from Blending-MgO might affect the proliferation of hPDLSCs.…”

Section: Discussionsupporting

confidence: 80%

“…After that, Mg 2+ was sustainably released for both Blending-MgO and Coaxial-MgO over 168 h, resulting in a stable Mg 2+ concentration around 5 mM. Numerous studies have shown that 5-10 mM Mg 2+ had a slight cytotoxic effect on cells and could promote cells' osteogenic differentiation (Mangalampalli et al, 2018;Onder et al, 2018). These results indicated that the MgOincorporated coaxial PCL/gelatin nanocellulose membranes with the sustainable release of Mg 2+ might be suitable for accelerating osteogenic differentiation of cells.…”

Section: Physicochemical Properties Of Pcl/gelatin Nanocellulose Membmentioning

confidence: 93%

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MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration

Peng

Ren

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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Electrospinning technique has attracted considerable attention in fabrication of cellulose nanofibrils or nanocellulose membranes, in which polycaprolactone (PCL) could be used as a promising precursor to prepare various cellulose nanofibril membranes for periodontal tissue regeneration. Conventional bio-membranes and cellulose films used in guided tissue regeneration (GTR) can prevent the downgrowth of epithelial cells, fibroblasts, and connective tissue in the area of tooth root but have limitations related to osteogenic and antimicrobial properties. Cellulose nanofibrils can be used as an ideal drug delivery material to encapsulate and carry some drugs. In this study, magnesium oxide (MgO) nanoparticles-incorporated PCL/gelatin core-shell nanocellulose periodontal membranes were fabricated using coaxial electrospinning technique, which was termed as Coaxial-MgO. The membranes using single-nozzle electrospinning technique, namely Blending-MgO and Blending-Blank, were used as control. The morphology and physicochemical property of these nanocellulose membranes were characterized by scanning electron microscopy (SEM), energy-dispersive spectrum of X-ray (EDS), transmission electron microscopy (TEM), contact angle, and thermogravimetric analysis (TGA). The results showed that the incorporation of MgO nanoparticles barely affected the morphology and mechanical property of nanocellulose membranes. Coaxial-MgO with core-shell fiber structure had better hydrophilic property and sustainable release of magnesium ion (Mg2+). CCK-8 cell proliferation and EdU staining demonstrated that Coaxial-MgO membranes showed better human periodontal ligament stem cells (hPDLSCs) proliferation rates compared with the other group due to its gelatin shell with great biocompatibility and hydrophilicity. SEM and immunofluorescence assay results illustrated that the Coaxial-MgO scaffold significantly enhanced hPDLSCs adhesion. In vitro osteogenic and antibacterial properties showed that Coaxial-MgO membrane enhanced alkaline phosphatase (ALP) activity, formation of mineralized nodules, osteogenic-related genes [ALP, collagen type 1 (COL1), runt-related transcription factor 2 (Runx2)], and high antibacterial properties toward Escherichia coli (E. coli) and Actinobacillus actinomycetemcomitans (A. a) when compared with controls. Our findings suggested that MgO nanoparticles-incorporated coaxial electrospinning PCL-derived nanocellulose periodontal membranes might have great prospects for periodontal tissue regeneration.

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Section: Discussionsupporting

confidence: 80%

Section: Physicochemical Properties Of Pcl/gelatin Nanocellulose Membmentioning

confidence: 93%

MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration

Peng

Ren

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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“…This positive effect was most probably associated, at least in part, to the Mg-particle composition, and this observation is in line with a variety of previous studies [ 19 , 20 , 21 , 22 , 48 ]. A recent review summarized the eventual underlying mechanisms, stressing that Mg ions appear to act in the all proliferation/osteoblastic differentiation pathway, by inducing early and late gene expression (Runx2, BMP-2, ALP, OCN, OPN) associated with a variety of osteogenic signaling pathways (integrin α2, integrin β1, FAK, ERK1/2, Notch1) [ 49 ], a process that is dose-dependent [ 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

et al. 2021

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Considering the role of magnesium in bone metabolism and the increasing relevance of plant-mediated green-synthesis, this work compares the bone cytocompatibility of magnesium hydroxide nanoparticles (NPs) produced by using pure water, Mg(OH)2, or a rosehip (RH) aqueous extract, Mg(OH)2RH. The NPs were evaluated for dose- and time-dependent effects on human osteoblastic and osteoclastic response, due to the direct involvement of the two cell types in bone metabolism. Mg(OH)2 NPs presented nanoplatelet-like morphology (mean diameter ~90 nm) and a crystalline structure (XRD analysis); the RH-mediated synthesis yielded smaller rounded particles (mean diameter <10 nm) with decreased crystallinity. On the ATR–FTIR spectra, both NPs presented the characteristic Mg-OH peaks; Mg(OH)2RH exhibited additional vibration bands associated with the presence of phytochemicals. On osteoblastic cells, NPs did not affect cell growth and morphology but significantly increased alkaline phosphatase (ALP) activity; on osteoclastic cells, particles had little effect in protein content, tartrate-resistant acid phosphatase (TRAP) activity, percentage of multinucleated cells, and cell area. However, compared with Mg(OH)2, Mg(OH)2RH increased osteoblastic differentiation by inducing ALP activity and promoting the expression of Runx2, SP7, Col1a1, and ALP, and had a negative effect on the expression of the osteoclastic genes NFATC1, CA2, and CTSK. These observations suggest the potential usefulness of Mg(OH)2RH NPs in bone regeneration.

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“…The elemental composition of Mg therefore correlated with the Ki-67 expression. In addition to this, it has been reported that Mg ions can promote the differentiation of mesenchymal stem cells to induce growth [ 44 , 45 ]. The elevated levels within the aggregoids could therefore be indicative of the matrix differentiation process in the mesenchymal cells across the tissue.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Osteosarcoma Aggregated Tumour Models with Human Tissue by Multimodal Mass Spectrometry Imaging

et al. 2021

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Osteosarcoma (OS) is the most common primary bone malignancy and largely effects adolescents and young adults, with 60% of patients under the age of 25. There are multiple cell models of OS described in vitro that express the specific genetic alterations of the sarcoma. In the work reported here, multiple mass spectrometry imaging (MSI) modalities were employed to characterise two aggregated cellular models of OS models formed using the MG63 and SAOS-2 cell lines. Phenotyping of the metabolite activity within the two OS aggregoid models was achieved and a comparison of the metabolite data with OS human tissue samples revealed relevant fatty acid and phospholipid markers. Although, annotations of these species require MS/MS analysis for confident identification of the metabolites. From the putative assignments however, it was suggested that the MG63 aggregoids are an aggressive tumour model that exhibited metastatic-like potential. Alternatively, the SAOS-2 aggregoids are more mature osteoblast-like phenotype that expressed characteristics of cellular differentiation and bone development. It was determined the two OS aggregoid models shared similarities of metabolic behaviour with different regions of OS human tissues, specifically of the higher metastatic grade.

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Magnesium doping on TiN coatings affects mesenchymal stem cell differentiation and proliferation positively in a dose-dependent manner

Cited by 17 publications

References 23 publications

MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration

MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration

Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

Comparison of Osteosarcoma Aggregated Tumour Models with Human Tissue by Multimodal Mass Spectrometry Imaging

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