Magnesium deficiency augments myocardial response to reactive oxygen species

Abstract

Magnesium (Mg) deficiency and oxidative stress are independently implicated in the etiopathogenesis of various cardiovascular disorders. This study was undertaken to examine the hypothesis that Mg deficiency augments the myocardial response to oxidative stress. Electrically stimulated rat papillary muscle was used for recording the contractile variation. Biochemical variables of energy metabolism (adenosine triphosphate (ATP) and creatine phosphate) and markers of tissue injury (lactate dehydrogenase (LDH) release and lipidperoxidation), which can affect myocardial contractility, were assayed in Langendorff-perfused rat hearts. Hydrogen peroxide (100 micromol/L) was used as the source of reactive oxygen species. The negative inotropic response to H2O2 was significantly higher in Mg deficiency (0.48 mmol Mg/L) than in Mg sufficiency (1.2 mmol Mg/L). Low Mg levels did not affect ATP levels or tissue lipid peroxidation. However, H2O2 induced a decrease in ATP; enhanced lipid peroxidation and the release of LDH were augmented by Mg deficiency. Increased lipid peroxidation associated with a decrease in available energy might be responsible for the augmentation of the negative inotropic response to H2O2 in Mg deficiency. The observations from this study validate the hypothesis that myocardial response to oxidative stress is augmented by Mg deficiency. This observation has significance in ischemia-reperfusion injury, where Mg deficiency can have an additive effect on the debilitating consequences.

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“…It has been reported that Mg deficiency enhanced the debilitating effects of ischemic injury [51] and stress sensitivity, which probably resulted in cardiovascular damage and arrhythmias [52]. It can also induce elevation of intracellular Ca 2+ concentrations, alterations in membrane permeability and transport processes in cardiac cells [50].…”

The bZIP transcription factor GmbZIP15 facilitates resistance against Sclerotinia sclerotiorum and Phytophthora sojae infection in soybean

“…To study whether the increased lipid peroxidation and ROS were caused by altered antioxidant activities, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were detected. These enzymes are important to plants for eliminating ROS ( Alscher et al., 2002 ; Manju and Nair, 2006 ). Under mock and pathogen infection conditions, SOD, POD, and CAT activities were much higher in OE plants than in WT plants ( Figures S2 A and S2B).…”

Effect of Feeding Enriched Mutton on Blood Lipid-Mineral Parameters and Cardio Vessel Changes in Male Sprague-Dawley Rats

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