Magnesium Corrosion Triggered Spontaneous Generation of H₂O₂ on Oxidized Titanium for Promoting Angiogenesis

Abstract

Although the use of reactive oxygen species (ROS) has been extensively studied, current systems employe xternal stimuli such as light or electrical energy to produce ROS,which limits their practical usage.Inthis report, biocompatible metals were used to construct anovel electrochemical system that can spontaneously generate H 2 O 2 without any external light or voltage.T he corrosion of Mg transfers electrons to Audecorated oxidized Ti in an energetically favorable process, and the spontaneous generation of H 2 O 2 in an oxygen reduction reaction was revealed to occur at titanium by combined spectroscopic and electrochemical analyses.T he controlled release of H 2 O 2 noticeably enhanced in vitro angiogenesis even in the absence of growth factors.F inally,an ew titanium implant prototype was developed by Mg incorporation, and its potential for promoting angiogenesis was demonstrated.

Double-edged effects caused by magnesium ions and alkaline environment regulate bioactivities of magnesium-incorporated silicocarnotite in vitro

“…This goal could also be achieved by Mg for its modulating microvascular functions in vivo [ 11 ]. Specifically, Mg-doped materials have proven to own enhanced angiogenic property in recent years, such as stimulating the production of reactive oxygen species, upregulating immune responses or promoting VEGF expression in human endothelial cells [ 12–14 ]. As a result, they have also been applied in vivo and achieved satisfying outcomes of vessel formation in animal models [ 15 , 16 ].…”

Development of durable microelectrodes for the detection of hydrogen peroxide and pH

“…It was also shown to have a limit of detection of 1 μM, which means that it is suitable for applications where μM concentrations of H 2 O 2 are expected. It is likely that cathodic stimulation of metals like titanium will result in μM levels of H 2 O 2 generation (Park et al, 2015). It was found that the sensitivity of the electrode was strongly influenced by the distance of the reference electrode from the sensing electrode.…”

Conceptual Study for Tissue-Regenerative Biodegradable Magnesium Implant Integrated with Nitric Oxide-Releasing Nanofibers

“…In particular, ROS has drawn considerable attention for its wound healing-related efficacy as a mediator of angiogenesis [28][29][30][31]. Thus, efforts to utilize ROS for biomedical applications were actively directed at introducing diverse ROS generation strategies such as natural release from donor materials [23,[32][33][34], external electric/photonic stimuli [35][36][37], and electrochemical reaction [38]. Indeed, we previously reported the promotion of angiogenesis induced by electrochemically generated hydrogen peroxide (H 2 O 2 ), one of the typical endogenous ROS [38] [23,[29][30][31], their practical use in biomedical applications has not been sufficiently developed due to the difficulties associated with establishing an appropriate storage/delivery system in vivo.…”