Magnesium concentration in plasma and erythrocytes in MS

Stelmasiak, Zbigniew; Solski, Janusz; Jakubowska, Beata

doi:10.1111/j.1600-0404.1995.tb00476.x

Cited by 14 publications

(2 citation statements)

References 18 publications

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“…Erythrocyte magnesium concentrations are performed with less time and effort and have become more popular. Direct and indirect measurements have been described (Al-Khursany, Thomas, Harrison, & Wilkinson, 1992;Corica, Allegra, Ientile, & Buemi, 1997;Elin & Hosseini, 1985;Guerin, Cousin, Mignot, Manchon, & Fournier, 1996;Gueux, Duchene-Marullaz, & Rayssiguier, 1988;Millart, Durlach, & Durlach, 1995;Moorkens, Vertommen, Meludu, Noe, & De Leeuw, 1997;Stelmasiak, Solski, & Jakubowska, 1995;Widmer, Féray et al, 1995;Widmer, Henrotte et al, 1995). For direct methods, erythrocytes are isolated from whole blood (Deuster, Trostmann, Bernier, & Dolev, 1987;Gueux et al, 1988;Millart et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Intracellular free magnesium concentration in healthy horses

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Merle

et al. 2018

Animal Physiology Nutrition

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Equine metabolic syndrome (EMS) is a worldwide disease in horses that parallels human diabetes mellitus type 2. In both diseases, patients show an altered peripheral insulin sensitivity as a key feature. In humans, multiple studies have demonstrated the beneficial effect of magnesium supplementation on insulin sensitivity. However, serum magnesium levels vary and are therefore not a reliable indicator of the patients' magnesium status. Determining the intracellular free magnesium concentration appears to be a more sensitive diagnostic indicator. In this study, the free intracellular magnesium concentration was measured using mag-fura 2 spectrophotometry in blood lymphocytes in 12 healthy, non-obese horses at 9 a.m., 12 a.m. and 4 p.m. to establish reference ranges according to a protocol designed for human blood lymphocytes. Additionally, the serum magnesium concentration was measured. In all horses, the total serum magnesium concentration was within the reference range. The mean free magnesium concentration in blood lymphocytes of all horses was 0.291 ± 0.067 mmol/L with no significant difference between the time points. The reference range for the free intracellular magnesium concentration in equine lymphocytes was set at 0.16-0.42 mmol/L. The established values are slightly lower than those in healthy humans. The designed protocol for the measurement of the intracellular free magnesium concentration might be an excellent research tool to assess the cellular magnesium status and to reliably diagnose an altered magnesium homeostasis in EMS. Further studies shall elucidate possible alterations in cellular magnesium status in horses with EMS.

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Section: Discussionmentioning

confidence: 99%

Intracellular free magnesium concentration in healthy horses

Winter

Sponder

Merle

et al. 2018

Animal Physiology Nutrition

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“…Long-lasting elevation in brain [Mg 2+ ] i occurs in some acute and chronic brain pathologies such as hypoxia/ischemia 13,14 and in patients with schizophrenia 15 . In contrast, [Mg 2+ ] i is reduced after traumatic brain injury 16,17 and in patients with Parkinson 18 , Alzheimer 19 , multiple sclerosis 20 , amyotrophic lateral sclerosis 21 , chronic migraine 22 and mitochondrial diseases 23 .…”

Section: Introductionmentioning

confidence: 99%

Molecular determinants of magnesium-dependent synaptic plasticity at electrical synapses formed by connexin36

et al. 2014

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Neuronal gap junction (GJ) channels composed of connexin36 (Cx36) play an important role in neuronal synchronization and network dynamics. Here we show that Cx36-containing electrical synapses between inhibitory neurons of the thalamic reticular nucleus are bi-directionally modulated by changes in intracellular free magnesium concentration ([Mg2+]i). Chimeragenesis demonstrates that the first extracellular loop of Cx36 contains a Mg2+-sensitive domain, and site-directed mutagenesis shows that the pore-lining residue D47 is critical in determining high Mg2+-sensitivity. Single channel analysis of Mg2+-sensitive chimeras and mutants reveals that [Mg2+]i controls the strength of electrical coupling mostly via gating mechanisms. In addition, asymmetric transjunctional [Mg2+]i induces strong instantaneous rectification, providing a novel mechanism for electrical rectification in homotypic Cx36 GJs. We suggest that Mg2+-dependent synaptic plasticity of Cx36-containing electrical synapses could underlie neuronal circuit reconfiguration via changes in brain energy metabolism that affects neuronal levels of intracellular ATP and [Mg2+]i.

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Neurotoxic Syndromes and Occupational Exposure to Solvents

Juntunen¹

1993

Environmental Research

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Magnesium concentration in plasma and erythrocytes in MS

Cited by 14 publications

References 18 publications

Intracellular free magnesium concentration in healthy horses

Intracellular free magnesium concentration in healthy horses

Molecular determinants of magnesium-dependent synaptic plasticity at electrical synapses formed by connexin36

Neurotoxic Syndromes and Occupational Exposure to Solvents

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