Magic Angle-Oriented Sample Spinning (MAOSS): A New Approach toward Biomembrane Studies

Glaubitz, Clemens; Watts, Anthony

doi:10.1006/jmre.1997.1344

Cited by 120 publications

(83 citation statements)

References 57 publications

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“…This is expected to improve the information content of the NMR spectroscopy of rotating oriented samples. 75 Preliminary results have been obtained. 105 …”

Section: γ -Encoded Double-quantum Recouplingmentioning

confidence: 98%

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Symmetry‐Based Pulse Sequences in Magic‐Angle Spinning Solid‐State NMR

Levitt

2003

ChemInform

181

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“…This is expected to improve the information content of the NMR spectroscopy of rotating oriented samples. 75 Preliminary results have been obtained. 105 …”

Section: γ -Encoded Double-quantum Recouplingmentioning

confidence: 98%

“…55,56,73 - 75 Glaubitz has proposed a series of different recoupling experiments, in order to deduce the angles subtended by internuclear vectors and the spinning axis with a minimum of ambiguity. 75 This should enhance the molecular structural information which may be obtained from the magic-angle spinning NMR of oriented samples.…”

Section: β-Dependencementioning

confidence: 99%

Symmetry‐Based Pulse Sequences in Magic‐Angle Spinning Solid‐State NMR

Levitt

2003

ChemInform

181

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“…75 This should enhance the molecular structural information which may be obtained from the magic-angle spinning NMR of oriented samples.…”

Section: β-Dependencementioning

confidence: 99%

Symmetry-Based Pulse Sequences in Magic-Angle Spinning Solid-State NMR

Levitt

2007

Encyclopedia of Magnetic Resonance

157

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“…The lipid/protein weight ratio was Ϸ1:1. Drops of Ϸ22 l containing Ϸ0.38 mg of protein were spread onto round, 0.01-mm thin glass plates with a diameter of 5.4 mm (Marienfeld GmbH, Lauda-Königshofen, Germany) according to a described procedure (37,38). The disks were mounted into clear 7-mm sapphire MAS rotors and rehydrated.…”

Section: N Photo-cidnp Mas Nmr On Ps2mentioning

confidence: 99%

¹⁵ N photochemically induced dynamic nuclear polarization magic-angle spinning NMR analysis of the electron donor of photosystem II

Diller

Roy

Gast

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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110

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In natural photosynthesis, the two photosystems that operate in series to drive electron transport from water to carbon dioxide are quite similar in structure and function, but operate at widely different potentials. In both systems photochemistry begins by photo-oxidation of a chlorophyll a, but that in photosystem II (PS2) has a 0.7 eV higher midpoint potential than that in photosystem I (PS1), so their electronic structures must be very different. Using reaction centers from 15 N-labeled spinach, these electronic structures are compared by their photochemically induced dynamic nuclear polarization (photo-CIDNP) in magic-angle spinning (MAS) NMR measurements. The results show that the electron spin distribution in PS1, apart from its known delocalization over 2 chlorophyll molecules, reveals no marked disturbance, whereas the pattern of electron spin density distribution in PS2 is inverted in the oxidized radical state. A model for the donor of PS2 is presented explaining the inversion of electron spin density based on a tilt of the axial histidine toward pyrrole ring IV causing -overlap of both aromatic systems.photosynthesis ͉ photosystem I ͉ solid-state NMR ͉ electron transfer ͉ redox potential I n photosynthesis, the two photosystems that operate in series to drive electron transport from water to carbon dioxide are similar in structure and function, but operate at widely different potentials. In both photosystems, photochemistry begins by photo-oxidation of a chlorophyll a (Chl). The oxidized electron donor of photosystem II (PS2) is the strongest oxidizing agent known in living nature, having a redox potential of ϩ 1.2V (1), required for water oxidation. The electronically excited donor of photosystem I (PS1), probably the most reducing compound in living nature (2), initiates the dark reaction. The question arises what factors tune those electronic properties. The spatial structure of PS2 (Fig. 1) shows two inner Chls (P D1 and P D2 ), two accessory Chls (Chl D1 and Chl D2 ), two pheophytin a (Phe) cofactors and two quinones in an arrangement similar to that in bacterial reaction centers (RCs) of purple bacteria.Photochemically induced dynamic nuclear polarization (photo-CIDNP) magic-angle spinning (MAS) NMR is an optical solidstate NMR method using the high electron polarization in the correlated electron pair and allows for strong increase of sensitivity and selectivity (4, 5) allowing to study the electronic structure of photosynthetic cofactors in great detail. The solid-state photo-CIDNP effect, discovered in 1994 (6), relies on different mechanisms called three-spin mixing (7), differential decay (8), and differential relaxation (9, 10). Recently, the contribution of these three mechanisms has been analyzed by field-dependent measurements on unlabeled RCs of the purple bacterium Rhodobacter sphaeroides (11,12). The chemical shift refers to the electronic ground-state after the photocycle, the photo-CIDNP signal intensity is linked to the intermediate radical state. Analytical expressions imply tha...

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Magic Angle-Oriented Sample Spinning (MAOSS): A New Approach toward Biomembrane Studies

Cited by 120 publications

References 57 publications

Symmetry‐Based Pulse Sequences in Magic‐Angle Spinning Solid‐State NMR

Symmetry‐Based Pulse Sequences in Magic‐Angle Spinning Solid‐State NMR

Symmetry-Based Pulse Sequences in Magic-Angle Spinning Solid-State NMR

¹⁵ N photochemically induced dynamic nuclear polarization magic-angle spinning NMR analysis of the electron donor of photosystem II

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Magic Angle-Oriented Sample Spinning (MAOSS): A New Approach toward Biomembrane Studies

Cited by 120 publications

References 57 publications

Symmetry‐Based Pulse Sequences in Magic‐Angle Spinning Solid‐State NMR

Symmetry‐Based Pulse Sequences in Magic‐Angle Spinning Solid‐State NMR

Symmetry-Based Pulse Sequences in Magic-Angle Spinning Solid-State NMR

15 N photochemically induced dynamic nuclear polarization magic-angle spinning NMR analysis of the electron donor of photosystem II

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¹⁵ N photochemically induced dynamic nuclear polarization magic-angle spinning NMR analysis of the electron donor of photosystem II