Maggots and wound healing: an investigation of the effects of secretions fromLucilia sericatalarvae upon interactions between human dermal fibroblasts and extracellular matrix components

Abstract

L. sericata larval secretions modify fibroblast adhesion and spreading across ECM protein surfaces, while keeping cells viable. Proteolytic activity of the ES played a significant role. If transferred to the wound situation, such alteration of fibroblast-ECM interactions may enhance new tissue formation.

The effects of Sarconesiopsis magellanica larvae (Diptera: Calliphoridae) excretions and secretions on fibroblasts

“…removing necrotic tissue (debridement) (Chambers et al, 2003;Sherman et al, 2000;Sherman and Shimoda, 2004), eliminating bacteria from a wound (Andersen et al, 2010;Bexfield et al, 2004) and stimulating the formation of granulation tissue, thereby accelerating wound cicatrisation (Horobin et al, 2005(Horobin et al, , 2006(Horobin et al, , 2003Prete, 1997). These properties have been associated with enzymatic activity, mainly from the serine-and metallo-protease families contained within Diptera ES (Chambers et al, 2003;El-Ebiarie and Taha, 2012;Horobin et al, 2005;Pinilla et al, 2013a).…”

“…Then 5000 cells were added per well and incubated for 24 h. ES were subsequently added at 5 g/mL and 1 g/mL concentrations and the plates were incubated for 24 or 48 h. ES which had been denatured for 1 h at 60 • C were added to the wells and observed by inverted microscope during each incubation period (Horobin et al, 2003); a photographic record was kept (10 photographs per well). The perimeter of each cell was then measured using a micrometre and specialised software (Image J).…”

“…Cell adhesion assays were done according to the protocol described by Horobin et al (2003), with some modifications. Polysorb 96-well plates were used; these had been pre-treated with 3 g/mL rat tail type I collagen (Sigma: C7661); 20,000 cells were then seeded in each well and incubated for 24 h; 5 g/mL or 1 g/mL (previously evaluated concentrations which had high cell viability) ES were then added and the plates were incubated for 4, 8, 24 and 48 h. Denatured larval ES were added which had been pre-heated for 1 h at 60 • C. The wells were washed three times with PBS (1×) following each incubation period; cells which did not remain adhered were discarded and the adhered cells were fixed with 2% paraformaldehyde for 30 min, washed with PBS (twice) and stained with crystal violet.…”

“…Modifying behaviour within a wound thus promotes new tissue formation (Chambers et al, 2003;Horobin et al, 2005Horobin et al, , 2006Horobin et al, , 2003. Even though native species (i.e.…”

Maggot Debridement Therapy in the Treatment of Chronic Wounds in a Military Hospital Setup in Turkey

“…Effective debridement of the wound re-establishes some of the normally occurring tissue repair progression such as clearance of dead tissue, angiogenesis, clearance of bacterial load and release of growth factors, thus facilitating granulation [19, 20]. It is not always possible to remove the necrotic tissue by surgical debridement without damaging healthy tissue [21].…”