Magainin-H2 effects on the permeabilization and mechanical properties of giant unilamellar vesicles

Mescola, Andrea; Marín-Medina, Nathaly; Ragazzini, Gregorio; Accolla, Maurizio; Alessandrini, Andrea

doi:10.1016/j.jcis.2019.06.028

Cited by 20 publications

(8 citation statements)

References 59 publications

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“…18 Another research direction is to explore the mechanical properties, such as local pressure and interfacial permeability, of symmetric phospholipid membranes via experimentations and simulations. [21][22][23][24][25] A previous experiment revealed the pressure response of cholesterol to solid-loaded phospholipid multilayers; it proved that cholesterol at a certain concentration decreases the tendency of separation and presents a new path for the construction of stable model membranes. 21 Meanwhile, a theoretical approach based on self-consistent eld theory has been developed to obtain the local stress across the membranes and interfaces in so matter; this approach predicts the membrane lateral stress prole in the regions of the head and tail and the stress between hydrophobic and hydrophilic interfaces.…”

Section: Introductionmentioning

confidence: 99%

Predicting asymmetric phospholipid microstructures in solutions

Shan

Wang

et al. 2020

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Predicting asymmetric phospholipid microstructures in solutions

Shan

Wang

et al. 2020

RSC Adv.

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“…Depending on peptide concentration, melittin pores can be promoted by positive or by negative curvature (88). In general, lipid shape and membrane curvature seem to modulate peptide partition, orientation, and penetration depth together with the local tilting of lipids, which are essential processes for the assembly of toroidal pores (88)(89)(90). Given the different effect of curved lipids on the activity of proteins/ peptides acting via toroidal pores, it is plausible that some pore properties such as size and peptide or lipid orientation could have an impact on the ability of negative and positive curved lipids to relocate into these pores (71,91).…”

Section: Discussionmentioning

confidence: 99%

Membrane Remodeling by the Lytic Fragment of SticholysinII: Implications for the Toroidal Pore Model

Mesa-Galloso

Valiente

Valdés‐Tresanco

et al. 2019

Biophysical Journal

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Sticholysins are pore-forming toxins of biomedical interest and represent a prototype of proteins acting through the formation of protein-lipid or toroidal pores. Peptides spanning the N-terminus of sticholysins can mimic their permeabilizing activity and, together with the full-length toxins, have been used as a tool to understand the mechanism of pore formation in membranes. However, the lytic mechanism of these peptides and the lipid shape modulating their activity are not completely clear. In this article, we combine molecular dynamics simulations and experimental biophysical tools to dissect different aspects of the pore-forming mechanism of StII 1-30 , a peptide derived from the N-terminus of sticholysin II (StII). With this combined approach, membrane curvature induction and flip-flop movement of the lipids were identified as two important membrane remodeling steps mediated by StII 1-30 . Pore formation by this peptide was enhanced by the presence of the negatively curved lipid phosphatidylethanolamine in membranes. This lipid emerged not only as a facilitator of membrane interactions but also as a structural element of the StII 1-30 pore that is recruited to the ring upon its assembly. Collectively, these, to our knowledge, new findings support a toroidal model for the architecture of the pore formed by StII 1-30 and provide new molecular insight into the role of phosphatidylethanolamine as a membrane component that can easily integrate into the ring of toroidal pores, thus probably aiding in their stabilization. This study contributes to a better understanding of the molecular mechanism underlying the permeabilizing activity of StII 1-30 and peptides or proteins acting via a toroidal pore mechanism and offers an informative framework for the optimization of the biomedical application of this and similar molecules.

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“…Several ways of exploiting the AMPs properties, such as Magainin H2, have a high level of hydrophobicity and present a good permeabilization action on lipid bilayers. Thus, experimental and theoretical studies investigated how AMPs and lipid bilayers interact, concluding that, at first, the lateral growth of the membrane caused by interactions with the AMP is inhibited, but in the end, it generates new bilayer regions that have defects in their membrane [69,70]. Another feasible option is the combined use of AMPs and conventional antibiotics, showing synergy and lowering microbial resistance [71].…”

Section: Antimicrobial Agents and Antimicrobial Resistancementioning

confidence: 99%

Preventing Biofilm Formation and Development on Ear, Nose and Throat Medical Devices

et al. 2021

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Otorhinolaryngology is a vast domain that requires the aid of many resources for optimal performance. The medical devices utilized in this branch share common problems, such as the formation of biofilms. These structured communities of microbes encased in a 3D matrix can develop antimicrobial resistance (AMR), thus making it a problem with challenging solutions. Therefore, it is of concern the introduction in the medical practice involving biomaterials for ear, nose and throat (ENT) devices, such as implants for the trachea (stents), ear (cochlear implants), and voice recovery (voice prosthetics). The surface of these materials must be biocompatible and limit the development of biofilm while still promoting regeneration. In this respect, several surface modification techniques and functionalization procedures can be utilized to facilitate the success of the implants and ensure a long time of use. On this note, this review provides information on the intricate underlying mechanisms of biofilm formation, the large specter of implants and prosthetics that are susceptible to microbial colonization and subsequently related infections. Specifically, the discussion is particularized on biofilm development on ENT devices, ways to reduce it, and recent approaches that have emerged in this field.

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Magainin-H2 effects on the permeabilization and mechanical properties of giant unilamellar vesicles

Cited by 20 publications

References 59 publications

Predicting asymmetric phospholipid microstructures in solutions

Predicting asymmetric phospholipid microstructures in solutions

Membrane Remodeling by the Lytic Fragment of SticholysinII: Implications for the Toroidal Pore Model

Preventing Biofilm Formation and Development on Ear, Nose and Throat Medical Devices

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