Maesa indica: a nutritional wild berry rich in polyphenols with special attention to radical scavenging and inhibition of key enzymes, α-amylase and α-glucosidase

As components of the human diet with potential health benefits, flavonols are the subject of numerous studies, confirming their antioxidant, free radical scavenging and anti-inflammatory activity. Taking into consideration the postulated pathogenesis of certain CNS dysfunctions characterized by neuronal degradation, flavonols may prevent the decay of neurons in multiple pathways. Leaves of Maesa membranacea yielded several flavonol glycosides including α-rhamnoisorobin (kaempferol 7-O-α-rhamnoside) and kaempferitrin (kaempferol 3,7-di-O-α-rhamnoside). The latter compound was a major constituent of the investigated plant material. Neuroprotective effects of kaempferitrin and α-rhamnoisorobin were tested in vitro using H2O2-, 6-OHDA- and doxorubicin-induced models of SH-SY5Y cell damage. Both undifferentiated and differentiated neuroblastoma cells were used in the experiments. α-Rhamnoisorobin at a concentration range of 1–10 µM demonstrated cytoprotective effects against H2O2-induced cell damage. The compound (at 1–10 µM) was also effective in attenuating 6-OHDA-induced neurotoxicity. In both H2O2- and 6-OHDA-induced cell damage, kaempferitrin, similar to isoquercitrin, demonstrated neuroprotective activity at the highest of the tested concentrations (50 µM). The tested flavonols were not effective in counteracting doxorubicin-induced cytotoxicity. Their caspase-3- and cathepsin D-inhibitory activities appeared to be structure dependent. Inhibition of the PI3-K/Akt pathway abolished the neuroprotective effect of the investigated flavonols.

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“…A. DC. [ 18 ]. Only two flavonoids, (-)-epicatechin and kaempferol, have been previously isolated from the stems of M. membranacea [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective Properties of Kempferol Derivatives from Maesa membranacea against Oxidative Stress-Induced Cell Damage: An Association with Cathepsin D Inhibition and PI3K/Akt Activation

Jantas

Malarz

et al. 2021

IJMS

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“…Certain ambiguities in the use of exact plant due to challenging disarrangement with reference to their different vernacular names for same species or different plant species is the major concern. [11,12] For example, E. ribes is being adulterate/substituted with E. tsjeriam-cottam, E. drupacea and M. indica and also various other drugs in the market, so genuineness of drug need to be assessed before use, as this may lead to the decrease in therapeutic efficacy. Hence, the availability of genuine sample of "Vidanga" needs immediate attention to maintain healthy therapeutic value.…”

Section: Original Articlementioning

confidence: 99%

Untitled

2021

IJGP

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Objective: There are various factors responsible for variations in the traded herbal composition on the basis of species variation, fruit immaturity, climatic conditions, substitutability, and uncertain trade practices. There is timely need to resolve this ambiguity. The present study designed to resolve the variation in traded fruits of "Vidanga." Materials and Methods: "Vidanga" fruits were collected from markets of different locations in India. The antioxidant activity was evaluated by 2,2-diphenyl-1-picryl-hydrazyl-hydrate, anti-lipid peroxidation (ALP), and ferric reducing antioxidant power (FRAP) using two different solvents, namely, ethanol and ethyl acetate. Total phenolic and flavonoid contents were studied by standard methods, namely, Folin-Ciocalteu and aluminum chloride respectively. Phytoconstituents investigation was also observed and chemical constituents were identified by liquid chromatography-mass spectrometry (LC-MS). Results and Discussion: Ethanolic and ethyl acetate extracts of "Vidanga" market samples exhibited highest total phenolic content in Pune sample (0.860 ± 0.003 mg/g gallic acid equivalent [GAE] equivalent) and in Nagpur (0.179 ± 0.015 mg/g quercetin equivalent), respectively. All the samples showed 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) scavenging activity in the order, highest activity in samples of Delhi followed by Nagpur, Mumbai, Bengaluru, and Pune. The ethyl acetate extract obtained from Nagpur sample had the strongest free radical-scavenging activity with IC 50 value of 89.54 μg/mL ± 0.001. ALP shows the order as lowest in Bengaluru sample and with slight increasing order followed by Nagpur, Delhi, Pune, and Mumbai samples which shows the high activity. Ethyl acetate extract of Pune sample showed the highest (40.47 ± 0.004 μg/mL) IC 50 . FRAP activity showed maximum inhibition in ethyl acetate extract of the Pune sample (67.76%) while the lowest activity showed in Delhi sample (33.64%). FRAP showed the lowest activity in Pune followed by Nagpur, Mumbai, Delhi, and high activity in Bengaluru sample. Phytochemical investigation shows the presence of alkaloids, carbohydrate, tannins, proteins, amino acids, fixed oils, gum and mucilage, glycosides, terpenoids, and steroids. LC-MS revealed phytoconstituent variations among all the samples. Conclusion: By evaluating commercial "Vidanga" samples from major markets, namely, Bengaluru, Delhi, Mumbai, Nagpur, and Pune, it is concluded that significant variation exists in the traded samples regarding their morphology, identity and medicinal attributes as studied from a string of biochemical assays as well as mass spectrum. Hence, the study suggests awareness in all scientific community, traders, practitioners, manufacturing units, pharmacies, etc., for an urgent need for the selection of authentic raw materials.

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“…Moreover, polyphenolic compounds from plants have also been reported to be good inhibitors of key enzymes of α-amylase and α-glucosidase associated with type 2 diabetes and these cause similar effects as of insulin in the utilization of glucose [13]. In our earlier publications, we reported that plant polyphenols function as potent inhibitors of α-amylase and α-glucosidase enzymes which decrease postprandial blood glucose levels [14,15]. Gupta et al [30] Passion fruit belongs to the genus Passiflora, which comprises about 580 plant species of the family Passifloraceae.…”

Section: Introductionmentioning

confidence: 99%

Potential of Passion (Passiflora spp.) Fruit in Control of Type II Diabetes

Narain

2018

CRDOJ

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Passiflora is one of the genera of Passifloracea family. Many of Passiflora fruits are edible and its other parts including leaves, seed and peel are used in traditional medicine system. Several Passiflora species are cherished for its pleasant aroma of the fruits which has been exploited by food, pharmaceutical and cosmetics industries. Presently, the main focus of utilizing this fruit is in its consumption as fruit juice or in manufacturing fruit products like juice, jam and jellies. The aim of this review is to present the current state of knowledge and research findings associated with the use of the Passiflora species in the control of type II diabetes.

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Maesa indica: a nutritional wild berry rich in polyphenols with special attention to radical scavenging and inhibition of key enzymes, α-amylase and α-glucosidase

Cited by 11 publications

References 32 publications

Neuroprotective Properties of Kempferol Derivatives from Maesa membranacea against Oxidative Stress-Induced Cell Damage: An Association with Cathepsin D Inhibition and PI3K/Akt Activation

Neuroprotective Properties of Kempferol Derivatives from Maesa membranacea against Oxidative Stress-Induced Cell Damage: An Association with Cathepsin D Inhibition and PI3K/Akt Activation

Untitled

Potential of Passion (Passiflora spp.) Fruit in Control of Type II Diabetes

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