2014
DOI: 10.1371/journal.pone.0115652
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Maduramicin Inhibits Proliferation and Induces Apoptosis in Myoblast Cells

Abstract: Maduramicin, a polyether ionophore antibiotic derived from the bacterium Actinomadura yumaensis, is currently used as a feed additive against coccidiosis in poultry worldwide. It has been clinically observed that maduramicin can cause skeletal muscle and heart cell damage, resulting in skeletal muscle degeneration, heart failure, and even death in animals and humans, if improperly used. However, the mechanism of its toxic action in myoblasts is not well understood. Using mouse myoblasts (C2C12) and human rhabd… Show more

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Cited by 23 publications
(22 citation statements)
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“…Several other mammalian tumor cells, including human rhabdomyosarcomas (RD) and mouse myoblasts (C2C12), have been reported to remain Ͼ80% viable after 72 h of treatment with 1 M maduramicin (29). The reason for the differential maduramicin sensitivities of apicomplexans, like P. falciparum and coccidia, and mammalian cells remains unknown, but it has allowed the use of maduramicin as a coccidiostat in animals.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…Several other mammalian tumor cells, including human rhabdomyosarcomas (RD) and mouse myoblasts (C2C12), have been reported to remain Ͼ80% viable after 72 h of treatment with 1 M maduramicin (29). The reason for the differential maduramicin sensitivities of apicomplexans, like P. falciparum and coccidia, and mammalian cells remains unknown, but it has allowed the use of maduramicin as a coccidiostat in animals.…”
Section: Resultsmentioning
confidence: 99%
“…The reason for the differential maduramicin sensitivities of apicomplexans, like P. falciparum and coccidia, and mammalian cells remains unknown, but it has allowed the use of maduramicin as a coccidiostat in animals. The standard dose used by the poultry industry for chicken feed is 5 to 7 mg/kg, which has been found to result in levels of maduramicin of ϳ100 nM in muscle, with no toxic effects on chickens or other nontarget species (29)(30)(31). Maduramicin treatment has a serum half-life of 13 h in chickens and does not interfere with vaccination responses, indicating that it did not affect the function of antigen-presenting cells or leukocyte activation and proliferation, which are required for the induction of immunity (31).…”
Section: Resultsmentioning
confidence: 99%
“…Cell viability was evaluated using CellTiter 96 ® AQ ueous One Solution Cell Proliferation Assay Kit (Promega), as described (Chen, Gu, Singh, Shang, Barzegar, Jiang and Huang 2014). Briefly, H9c2 cells suspended in the growth medium were seeded in a 96-well plate at a density of 5 × 10 3 cells/well (in triplicates) and grown overnight.…”
Section: Methodsmentioning
confidence: 99%
“…Cell death was evaluated by the trypan blue exclusion assay as described (Chen, Gu, Singh, Shang, Barzegar, Jiang and Huang 2014). Briefly, cells were seeded in 100-mm dishes at a density of 3 × 10 5 cells per dish in the growth medium and grown overnight.…”
Section: Methodsmentioning
confidence: 99%
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