MADS box genes expressed in developing inflorescences of rice and sorghum

Greco, Raffaella; Stagi, L; Colombo, Lucia; Angenent, Gerco C.; Sari‐Gorla, M.; Pè, Mario Enrico

doi:10.1007/s004380050364

Cited by 68 publications

(43 citation statements)

References 33 publications

(38 reference statements)

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“…The WSEP homoeologs belong to the Os MADS45-type cluster, and the WLHS1 homoeologs belong to the Os MADS1-type cluster. In the Os MADS45-type cluster, there are two rice genes, Os MADS24 and Os MADS45 (Greco et al, 1997). WSEP genes formed a subcluster with Os MADS45 that was distinct from Os MADS24.…”

Section: Identification Of Class E Mads Box Genes In Wheatmentioning

confidence: 99%

Genetic and Epigenetic Alteration among Three Homoeologous Genes of a Class E MADS Box Gene in Hexaploid Wheat

et al. 2007

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Bread wheat (Triticum aestivum) is a hexaploid species with A, B, and D ancestral genomes. Most bread wheat genes are present in the genome as triplicated homoeologous genes (homoeologs) derived from the ancestral species. Here, we report that both genetic and epigenetic alterations have occurred in the homoeologs of a wheat class E MADS box gene. Two class E genes are identified in wheat, wheat SEPALLATA (WSEP) and wheat LEAFY HULL STERILE1 (WLHS1), which are homologs of Os MADS45 and Os MADS1 in rice (Oryza sativa), respectively. The three wheat homoeologs of WSEP showed similar genomic structures and expression profiles. By contrast, the three homoeologs of WLHS1 showed genetic and epigenetic alterations. The A genome WLHS1 homoeolog (WLHS1-A) had a structural alteration that contained a large novel sequence in place of the K domain sequence. A yeast two-hybrid analysis and a transgenic experiment indicated that the WLHS1-A protein had no apparent function. The B and D genome homoeologs, WLHS1-B and WLHS1-D, respectively, had an intact MADS box gene structure, but WLHS1-B was predominantly silenced by cytosine methylation. Consequently, of the three WLHS1 homoeologs, only WLHS1-D functions in hexaploid wheat. This is a situation where three homoeologs are differentially regulated by genetic and epigenetic mechanisms.

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Section: Identification Of Class E Mads Box Genes In Wheatmentioning

confidence: 99%

Genetic and Epigenetic Alteration among Three Homoeologous Genes of a Class E MADS Box Gene in Hexaploid Wheat

et al. 2007

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“…OsMADS45 shares homology with Arabidopsis SEP1 (AGL2) and SEP2 (AGL4) (Pelaz et al, 2000) and was shown to be expressed in floral organs in whorl 2, 3 and 4 of wild-type flowers (Greco et al, 1997) (Fig. 7C).…”

Section: Interaction Between Spw1 and DLmentioning

confidence: 99%

SUPERWOMAN1 and DROOPING LEAFgenes control floral organ identity in rice

et al. 2003

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We analyzed recessive mutants of two homeotic genes in rice, SUPERWOMAN1 (SPW1) and DROOPING LEAF (DL). The homeotic mutation spw1 transforms stamens and lodicules into carpels and palea-like organs, respectively. Two spw1 alleles, spw1-1 and spw1-2, show the same floral phenotype and did not affect vegetative development. We show that SPW1 is a rice APETALA3 homolog, OsMADS16. In contrast, two strong alleles of the dl locus, drooping leafsuperman1 (dl-sup1) and drooping leaf-superman2 (dlsup2), cause the complete transformation of the gynoecium into stamens. In these strong mutants, many ectopic stamens are formed in the region where the gynoecium is produced in the wild-type flower and they are arranged in a non-whorled, alternate pattern. The intermediate allele dl-1 (T65), results in an increase in the number of stamens and stigmas, and carpels occasionally show staminoid characteristics. In the weakest mutant, dl-2, most of the flowers are normal. All four dl alleles cause midrib-less drooping leaves. The flower of the double mutant, spw1 dl-sup, produces incompletely differentiated organs indefinitely after palea-like organs are produced in the position where lodicules are formed in the wild-type flower. These incompletely differentiated organs are neither stamens nor carpels, but have partial floral identity. Based on genetic and molecular results, we postulate a model of stamen and carpel specification in rice, with DL as a novel gene controlling carpel identity and acting mutually and antagonistically to the class B gene, SPW1.

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“…OsMADS1 (Chung et al, 1994), ZMM3, ZMM6 , OsMADS24, SbMADS1 (Greco et al, 1997), TM5 (Pnueli et al, 1991), FBP2 (Angenent et al, 1994), AGL2 (Ma et al, 1991) and ZMM2 b After Fischer et al (1995). Fig.…”

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“…In monocots, MADS box genes have been cloned from orchid (Lu et al, 1993), maize (Schmidt et al, 1993), rice (Chung et al, 1994), and sorghum (Greco et al, 1997). However, little is known about the functions of MADS box genes in monocots, although the MADS box genes are believed to play substantial roles on floral organ identity.…”

mentioning

confidence: 99%

Wheat MADS box genes, a multigene family dispersed throughout the genome.

Murai¹,

Murai²,

Ogihara

1997

Genes Genet. Syst.

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Polymerase chain reaction (PCR) with degenerate primers was utilized for partial cloning of the MADS box gene family from wheat (Triticum aestivum L.). PCR products corresponding to a part of the MADS box region were cloned and sequenced. Eleven individual clones sequenced were classified into seven types on the basis of the nucleotide sequence and five types on the deduced amino acid sequence, which included two wheat-specific MADS box protein sequences. RT-PCR analysis with degenerate primers revealed preferential expression of the MADS box genes in young spikes. Furthermore, genomic Southern blot analysis with degenerate PCR products as probes indicated that wheat MADS box genes constitute a multigene family and are dispersed throughout the genome.

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MADS box genes expressed in developing inflorescences of rice and sorghum

Cited by 68 publications

References 33 publications

Genetic and Epigenetic Alteration among Three Homoeologous Genes of a Class E MADS Box Gene in Hexaploid Wheat

Genetic and Epigenetic Alteration among Three Homoeologous Genes of a Class E MADS Box Gene in Hexaploid Wheat

SUPERWOMAN1 and DROOPING LEAFgenes control floral organ identity in rice

Wheat MADS box genes, a multigene family dispersed throughout the genome.

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