MaDREB1F confers cold and drought stress resistance through common regulation of hormone synthesis and protectant metabolite contents in banana

Abstract

Adverse environmental factors severely affect crop productivity. Improving crop resistance to multiple stressors is an important breeding goal. Although CBFs/DREB1s extensively participate in plant resistance to abiotic stress, the common mechanism underlying CBFs/DREB1s that mediate resistance to multiple stressors remains unclear. Here, we show the common mechanism for MaDREB1F conferring cold and drought stress resistance in banana. MaDREB1F encodes a dehydration responsive element binding protein (DREB) transcription factor with nuclear localization and transcriptional activity. MaDREB1F expression is significantly induced after cold, osmotic, and salt treatments. MaDREB1F overexpression increases banana resistance to cold and drought stress by commonly modulating the protectant metabolite levels of soluble sugar and proline, activating the antioxidant system, and promoting jasmonate and ethylene syntheses. Transcriptomic analysis shows that MaDREB1F commonly activates or alleviates the repression of jasmonate and ethylene biosynthetic genes under cold and drought conditions. Moreover, MaDREB1F directly activates the promoter activities of MaAOC4 and MaACO20 for jasmonate and ethylene syntheses, respectively, under cold and drought conditions. MaDREB1F also targets the MaERF11 promoter to activate MaACO20 expression for ethylene synthesis under drought stress. Together, our findings offer new insight into the common mechanism underlying CBFs/DREB1s-mediated cold and drought stress resistance, which has substantial implications for engineering cold- and drought- tolerant crops.

Genome-Wide Identification and Expression Profiles of C-Repeat Binding Factor Transcription Factors in Betula platyphylla under Abiotic Stress

“…Additionally, CBF transcription factors also play important roles in other abiotic stress responses, such as salt and drought stress. In banana ( Musa acuminata ), CBF transcription factor MaDREB1F was responsive to salt and osmotic treatment and expressed at the highest level at 24 d, 17 d, respectively [ 26 ]. Overexpression of MaDREB1F in banana enhanced drought tolerance of the transgenic plants.…”

Discovery of gene regulation mechanisms associated with uniconazole-induced cold tolerance in banana using integrated transcriptome and metabolome analysis

“…T indicates the uniconazole treatment of 0.2 g. CK indicates the water control treatment plantain and promotes glucose decomposition [15,33]. The soluble sugar content affects cold tolerance in plants [34], while other plant hormones such as ABA, ethylene, and jasmonate have also been reported to regulate plant cold resistance [11,32,35]. As a GA inhibitor, uniconazole can alter the content of IAA, ABA, and other hormones in plants [36,37].…”

“…Cold tolerance in banana plants is enhanced by increasing the levels of compatible solutes such as proline, soluble carbohydrates, and phenolic compounds [1,2]. Physiological processes, such as antioxidant capacity, photosynthesis, and plant hormone pathways, also tend to change in response to cold [5,[9][10][11].…”

“…MAPK3-ICE1-POD P7 pathway can significantly change the expression of coldresponsive genes and the oxidoreductase activity, PIP1;1 can reduce ion leakage and malondialdehyde content, as well as increase proline, chlorophyll, soluble sugar, and abscisic acid (ABA) content [9,14]. The overexpression of dehydration-responsive element binding protein 1 F (DREB1F) enhances banana resistance to cold stress by regulating the levels of protectant metabolites of soluble sugars and proline, activating the antioxidant system, and promoting jasmonate and ethylene synthesis [11].…”

A review on adaptation of banana (Musa spp.) to cold in subtropics

“…Superoxide dismutase encoding gene ( sod ) responds to cold stress by dismutation of ROS and its intermediate (Gupta et al, 1993; Zhou et al, 2019). A cold inducible zinc finger protein SCOF1 acts as transcription factor of SGBF‐1 which activates the COR gene expression and improves the cold tolerance in plants (Kim et al, 2001; Xu et al, 2022). Some stress‐inducible protein such as chloroplast stroma protein ( Cor15am ) has cryoprotective activity against ROS and its intermediates (Nakayama et al, 2007).…”