“…Such comparisons would not be feasible using bulk RNA-sequencing, because reads in bulk RNA-seq data may correspond to distinct DNA lesions in different cells. This scRNA-seq strategy has recently been used in another study [ 21 ], in which arrested yeast cells and quiescent mouse neural stem cells (NSCs) were exposed to N -methyl- N ′-nitro- N -nitrosoguanidine (MNNG), a chemical compound that is routinely used to induce O 6 -mG DNA lesions and RNA synthesis errors [ 16 , 22 ], and subsequently used as input material for scRNA-seq. It was found that certain transcription errors do indeed occur repetitively as a consequence of MNNG exposure.…”