Macroscopical and Histological Aspects of the Cerebellum in Chinchillas

Irimescu, Irina; Bolfă, Pompei; Crişan, M.; Dezdrobitu, Cristian; Damian, Aurel

doi:10.1016/j.aaspro.2015.08.093

Cited by 3 publications

(2 citation statements)

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“…The cerebellum is involved in cognitive processes in humans, including language, learning, and memory (Ito, 1993), and differences in cerebellar volume, histology, and morphology appear to correspond with the performance of other tasks, as some differences are known to be based on species, behavioral features, and general anatomic structure (Iwaniuk, Hurd, & Wylie, 2006;Miall & Reckess, 2002;Mondal, 2003). When evaluating the histo-architectural data concerning the three cerebellar lamellar structures, we concur with the descriptions in literature regarding mammals in general (Neary, 2008) and rodents (Irimescu, Bolf a, Cris ¸an, Dezdrobitu, & Damian, 2015;Paxinos, 2004;Ruigrok, Sillitoe, & Voogd, 2015). The human cerebral cortex consists of six layers: the external granular, external pyramidal, internal granular, internal pyramidal, multiform (fusiform), and molecular (plexiform) layers (Fatterpekar et al, 2002).…”

Section: Discussionsupporting

confidence: 87%

Outer and inner mitochondrial membrane proteins TOMM40 and TIMM50 are intensively concentrated and localized at Purkinje and pyramidal neurons in the New Zealand white rabbit brain

Farouk

Abdellatif

Metwally

2021

The Anatomical Record

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Mitochondria are involved in a variety of developmental processes and neurodegenerative diseases. The translocase complexes of the outer and inner mitochondrial membranes (TOM and TIM) are protein complexes involved in transporting protein precursors across mitochondrial membranes. Although rabbits are important animal models for neurodegenerative diseases, the expression of TOM and TIM complexes has yet to be examined in the rabbit brain. In the present study, we quantitatively evaluated the protein expression of the translocase of outer mitochondrial membrane 40 (TOMM40) and inner mitochondrial membrane 50 (TIMM50) complexes, two of the TOM/TIM complexes, in the cerebral, cerebellar, and hippocampal cortices of the New Zealand white rabbit brain, using immunohistochemistry. Sections from brain specimens were initially stained for cytochrome c oxidase (COX), a wellknown mitochondrial marker, which was found to be homogeneously expressed in the cerebrum, but localized to the Purkinje and pyramidal neurons of the cerebellum and hippocampus, respectively. TOMM40 and TIMM50 proteins consistently revealed a similar expression pattern, although at different ratios. In the cerebrum, TOMM40 and TIMM50 immunoreactions were homogeneously distributed within the cytoplasm of various neurons. Meanwhile, Purkinje cells in the cerebellum and pyramidal neurons in the hippocampus displayed higher intensities in their cytoplasm. The specific cellular localization of TOMM40 and TIMM50 proteins in various regions of the rabbit brain suggests a distinct function of each protein in these regions. Further analysis will be required to evaluate the molecular functions of these proteins.

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Section: Discussionsupporting

confidence: 87%

Outer and inner mitochondrial membrane proteins TOMM40 and TIMM50 are intensively concentrated and localized at Purkinje and pyramidal neurons in the New Zealand white rabbit brain

Farouk

Abdellatif

Metwally

2021

The Anatomical Record

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“…The chinchilla is an experimental model for conducting surgical, toxicological and pharmacological studies (Irimescu, Bolfă, Crişan, et al., 2015; Irimescu, Bolfă, Crișan, et al., 2015; Otto et al., 2008).…”

Section: Introductionmentioning

confidence: 99%

Computed tomography and three dimensional anatomical study of the liver in the chinchilla (Chinchilla lanigera)

Dilek,

Dimitrov,

Stamatova‐Yovcheva

et al. 2024

Anat Histol Embryol

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Few instances of neoplastic formations in the liver of chinchillas have been found, even though the species is widely used in different scientific experiments. In the present article we investigate the anatomical features of the chinchilla's liver using CT and three dimension (3D) imaging. For the trials we used 12 (six males and six females) clinically healthy chinchillas all at 18 months of age. The animals were positioned in dorsal recumbency. We used Th8 to L2 vertebrae and the sternum as bone markers for the transverse CT study. The investigated anatomical landmarks for the CT coronal study were the vertebrae, costal arch, soft abdominal wall, diaphragm, stomach and the right kidney. 3D reconstructions were accomplished with a specific imaging software. On transverse and coronal CT images, the chinchilla's liver was composed of lobus hepatis sinister lateralis, ‘middle lobe’–without proper Latin term in NAV 2017, lobus hepatis dexter and lobus caudatus. The ‘middle lobe’ was separated into the ‘left middle lobe’ and the ‘right middle lobe’. Lobus hepatis dexter consisted of lobus hepatis dexter medialis and lobus hepatis dexter lateralis. There was an anatomical relation between the liver, fundus ventriculi and corpus ventriculi. Proc. caudatus was in close contact with the right kidney. Vesica fellea was elongated and ellipsoid. 3D reformatted images confirmed the results obtained by transverse and coronal CT studies. The CT density of the liver in HU was 195.6 ± 73.1. The CT and 3D reconstructed images were visualized at high resolution. This data could be used as a basis for further morphological and imaging studies.

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