Macroporous Composite Cryogels with Embedded Polystyrene Divinylbenzene Microparticles for the Adsorption of Toxic Metabolites from Blood

Eichhorn, Tanja; Ivanov, Alexander E.; Dainiak, Maria B.; Leistner, André; Linsberger, Ingrid; Jungvid, Hans; Mikhalovsky, Sergey V.; Weber, Viktoria

doi:10.1155/2013/348412

Cited by 16 publications

(8 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This idea of incorporating particles into hydrogel for detoxification purposes has been demonstrated by early studies454647. We propose to shape the PEG matrix encapsulated with PDA nanoparticles into a designer structure using 3D printing, a disruptive technology that is finding promising applications in creating unique 3D devices and tissue constructs4849.…”

Section: Discussionmentioning

confidence: 99%

Bio-inspired detoxification using 3D-printed hydrogel nanocomposites

Gou¹,

et al. 2014

View full text Add to dashboard Cite

Rationally designed nanoparticles that can bind toxins show great promise for detoxification. However, the conventional intravenous administration of nanoparticles for detoxification often leads to nanoparticle accumulation in the liver, posing a risk of secondary poisoning especially in liver-failure patients. Here we present a liver-inspired three-dimensional (3D) detoxification device. This device is created by 3D printing of designer hydrogels with functional polydiacetylene nanoparticles installed in the hydrogel matrix. The nanoparticles can attract, capture and sense toxins, while the 3D matrix with a modified liver lobule microstructure allows toxins to be trapped efficiently. Our results show that the toxin solution completely loses its virulence after treatment using this biomimetic detoxification device. This work provides a proof-of-concept of detoxification by a 3D-printed biomimetic nanocomposite construct in hydrogel, and could lead to the development of alternative detoxification platforms.

show abstract

Section: Discussionmentioning

confidence: 99%

Bio-inspired detoxification using 3D-printed hydrogel nanocomposites

Gou¹,

et al. 2014

View full text Add to dashboard Cite

show abstract

“…To ensure adhesion of the cryogels to the plate wells, these were first filled by 20 μL 2.5% w/v PVA solution in 50% aqueous acetone, which evaporated under gentle heating on an electric plate, forming a thin transparent PVA coating; PVA is known to adhere well to polystyrene [23]. Then 40 μL of the PVA-GA reaction mixture made as described in Section 2.2.1 was added to the wells, frozen at -18 °C and kept overnight.…”

Section: Methodsmentioning

confidence: 99%

Thin poly(vinyl alcohol) cryogels: reactive groups, macropores and translucency in microtiter plate assays

Ivanov

Ljunggren

2019

Heliyon

Self Cite

View full text Add to dashboard Cite

Thin macroporous poly(vinyl alcohol) (PVA) hydrogels were produced by cross-linking of PVA in a semi-frozen state with glutaraldehyde (GA) on glass slides or in the wells of microtiter plates. The 100-130 μm-thick gels were mechanically transferable, squamous translucent films with a high porosity of 7.2 ± 0.3 mL/g dry PVA i.e. similar to larger cylindrical PVA monoliths of the same composition. Additional treatment of the gels with 1% GA increased the aldehyde group content from 0.7 to 2.4 μmol/mL as estimated using dinitrophenylhydrazine (DNPH) reagent. Translucency of the gels allowed registration of UV-visible spectra of the DNPH-stained films. The catalytic activity of trypsin covalently immobilized on thin gels in the microtiter plates was estimated with chromogenic substrate directly in the wells, and indicated that the amount of protein immobilized was at least 0.34 mg/mL gel. Human immunoglobulin G (IgG) immobilized on thin gels at 0.1–10 mg/mL starting concentrations could be detected in a concentration-dependent manner due to recognition by anti-human rabbit IgG conjugated with peroxidase and photometric registration of the enzymatic activity. The results indicate good permeability of the hydrogel pores for macromolecular biospecific reagents and suggest applications of thin reactive PVA hydrogels in photometric analytical techniques.

show abstract

“…Specifically, cryogels have shown promise as vehicles for both the storage and transportation of cells (Vrana et al, 2012;Katsen-Globa et al, 2014;Borg et al, 2016). Beyond storage, cryogels also have the potential to be used as bioreactors, with the surface area-to-volume ratio proving ideal for culturing large quantities of cells Çimen & Denizli, 2012;Eichhorn et al, 2013). For example, in a study by Jain et al (2015) two different cryogels of copolymers acrylonitrile (AN) and N-vinyl-2-pyrrolidone (NVP) (poly(AN-co-NVP)), as well as chitosan and poly(N-isopropylacrylamide) (poly(NiPAAm)chitosan), were fabricated.…”

Section: Bioreactormentioning

confidence: 99%

Three‐dimensional cryogels for biomedical applications

Razavi

Qiao

Thakor

2019

J Biomedical Materials Res

View full text Add to dashboard Cite

Cryogels are a subset of hydrogels synthesized under sub‐zero temperatures: initially solvents undergo active freezing, which causes crystal formation, which is then followed by active melting to create interconnected supermacropores. Cryogels possess several attributes suited for their use as bioscaffolds, including physical resilience, bio‐adaptability, and a macroporous architecture. Furthermore, their structure facilitates cellular migration, tissue‐ingrowth, and diffusion of solutes, including nano‐ and micro‐particle trafficking, into its supermacropores. Currently, subsets of cryogels made from both natural biopolymers such as gelatin, collagen, laminin, chitosan, silk fibroin, and agarose and/or synthetic biopolymers such as hydroxyethyl methacrylate, poly‐vinyl alcohol, and poly(ethylene glycol) have been employed as 3D bioscaffolds. These cryogels have been used for different applications such as cartilage, bone, muscle, nerve, cardiovascular, and lung regeneration. Cryogels have also been used in wound healing, stem cell therapy, and diabetes cellular therapy. In this review, we summarize the synthesis protocol and properties of cryogels, evaluation techniques as well as current in vitro and in vivo cryogel applications. A discussion of the potential benefit of cryogels for future research and their application are also presented.

show abstract

Macroporous Composite Cryogels with Embedded Polystyrene Divinylbenzene Microparticles for the Adsorption of Toxic Metabolites from Blood

Cited by 16 publications

References 25 publications

Bio-inspired detoxification using 3D-printed hydrogel nanocomposites

Bio-inspired detoxification using 3D-printed hydrogel nanocomposites

Thin poly(vinyl alcohol) cryogels: reactive groups, macropores and translucency in microtiter plate assays

Three‐dimensional cryogels for biomedical applications

Contact Info

Product

Resources

About