Macroporous chitosan hydrogels: Effects of sulfur on the loading and release behaviour of amino acid-based compounds

Elviri, Lisa; Asadzadeh, Maliheh; Cucinelli, Roberta; Bianchera, Annalisa; Bettini, Ruggero

doi:10.1016/j.carbpol.2015.06.048

Cited by 26 publications

(20 citation statements)

References 33 publications

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“…It has been frequently reported that freeze‐drying process can be used for the fabrication of tissue engineering hydrogels . Although these studies indicated that direct extrapolation on the microstructural characteristics of the hydrogels in hydrated form is not fully possible, SEM observations remain interesting since these artifacts resemble the structures of the hydrated gels . With high magnification micrographs, the pore walls were dendritic, providing suitable surfaces for the attachment of cells to the pores (Figure ).…”

Section: Resultsmentioning

confidence: 99%

Ionically Crosslinked Thermoresponsive Chitosan Hydrogels formed In Situ: A Conceptual Basis for Deeper Understanding

Rahmati

Milan

Samadikuchaksaraei

et al. 2017

Macro Materials & Eng

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In situ formation and the performance of ionically crosslinked thermosensitive chitosan‐based hydrogels are presented. Experimental analyses, together with mechanistic descriptions of the events during hydrolysis, are employed to uncover the role of urea and isobutanol as chemical modifiers by comparing three classes of hydrogels formed in chitosan/β‐glycerolphosphate (β‐GP) solutions. Rheological measurements demonstrate that urea caused an increase in gelation time and temperature of chitosan/β‐GP systems, while isobutanol has an inverse effect. Interpretations based on increase in pH and chemical bonding of components in chitosan solutions provide further insight into hydrogel network formation. Urea can hinder the hydrophobic characteristics of chitosan‐based hydrogels, whereas isobutanol has the opposite effect. The shape retaining strategy applied here helps in simulation and interpretation of performance of thermosensitive hydrogels for biomedical purposes.

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Section: Resultsmentioning

confidence: 99%

Ionically Crosslinked Thermoresponsive Chitosan Hydrogels formed In Situ: A Conceptual Basis for Deeper Understanding

Rahmati

Milan

Samadikuchaksaraei

et al. 2017

Macro Materials & Eng

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“…In order to improve the ability of chitosan to act as a substrate for cell growth, the preparation of chitosan films in the presence of phosphates and D‐(+)‐raffinose was described, which positively contributed to the growth of WI‐38 and HUVEC cells on this biomaterial. The same model however was not very efficient in supporting the growth of osteoblastic cells, so further functionalization of these hydrogels was performed to improve their biocompatibility by exploiting the binding affinity of chitosan for sulfur‐containing components, such as thiol‐modified gelatin, or by means of aptamers …”

Section: Introductionmentioning

confidence: 99%

“…The same model however was not very efficient in supporting the growth of osteoblastic cells, so further functionalization of these hydrogels was performed to improve their biocompatibility by exploiting the binding affinity of chitosan for sulfur-containing components, 15 such as thiol-modified gelatin, 16 or by means of aptamers. 17 A previous work by Parisi et al 17 described the positive effect of the functionalization of chitosan films with anti-fibronectin aptamers on their colonization by murine osteoblastic cells.…”

Section: Introductionmentioning

confidence: 99%

Surface modification of chitosan films with a fibronectin fragment–DNA aptamer complex to enhance osteoblastic cell activity: A mass spectrometry approach probing evidence on protein behavior

Saccani

Parisi

Bergonzi

et al. 2019

Rapid Comm Mass Spectrometry

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Rationale Protein dynamics are fundamental for biological activity. Direct surface functionalization with these biomolecules often creates regions presenting non‐homogeneous and altered protein functionality. Aptamers have been shown to be an effective method to enhance the biocompatibility of biomaterials by acting as docking points for biomolecules capable of improving cell responses and the colonization/proliferation processes. Here mass spectrometry (MS) was successfully applied as an analytical approach to study the interactions between a fibronectin fragment (FN) and anti‐fibronectin aptamers in solution and on a chitosan film. Methods A 30 kDa N‐terminal fibronectin fragment, a ssDNA anti‐fibronectin 40 nucleotide long aptamer and chitosan 95/50 (degree of deacetylation 92.6–97.5%; molecular weight 80–220 kDa) were used. First, the dynamics of the FN in aqueous solution, in the presence or absence of anti‐FN‐specific DNA aptamers, were described. Thus, the same study was carried out on 2% (w/w) chitosan‐based films, functionalized with FN alone or through aptamers as selective spacers. Results Amide hydrogen/deuterium exchange mass spectrometry (HDX‐MS) analysis identified the SYRIGDTWSKKDNRGNL and YRVGDTYERPKDSMI, YVVGETWEKPYQGWMM and WERTYLGNAL fragments as presumably involved in the interaction of FN with aptamers. MS findings indicated the improved functionality of FN on chitosan when aptamers were used as selective spacer, and this may explain why cells preferentially attached to aptamer‐bound FN rather than on chitosan‐FN films. Conclusions Aptamers did not affect the amount of adsorbed FN, but influenced its conformation enhancing its biological activity toward adhesion and growth of osteoblasts. HDX‐MS data allowed the identification of the FN/aptamer interaction regions. Differences in FN flexibility on the chitosan film in the presence or absence of the aptamer were established providing insights at the molecular level to better understand the protein biological functionality on cell proliferation.

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“…However, some cell types grow slowly on chitosan films, and consequently they were chosen as substrates to be enriched with aptamers. Aptamers were immobilized on 2% chitosan films (r = 3.0 mm; h = 0.25 mm) at increasing concentration by exploiting the spontaneous ability of chitosan to bind sulfur-containing substances [147]. Five thousand hOB cells (human osteoblasts) on tHA/PEGDA gels and 5000 MC3T3-E1 cells (murine preosteoblasts from bone/calvaria) on 2% chitosan films were cultured for 7 days.…”

Section: Aptamers Enhance Cell Adhesion and Proliferation On Polymerimentioning

confidence: 99%

Aptamer-Mediated Selective Protein Affinity to Improve Scaffold Biocompatibility

Parisi¹,

Galli²,

Elviri³

et al. 2016

Advanced Techniques in Bone Regeneration

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Protein adsorption on surfaces occurs shortly after scaffold insertion. This process is of pivotal importance to achieve therapeutic success in tissue engineering (TE), and favorable proteins should be adsorbed at the interface without unfolding to preserve their structure and function. Protein misfolding at the interface is a common phenomenon, which can impair cell adhesion and scaffold colonization. Many efforts have been done to improve scaffold biocompatibility by ameliorating protein adsorption, but with poor results. In the present chapter, we propose the use of a novel class of molecules, aptamers, to improve scaffold biocompatibility. Aptamers are small, single stranded oligonucleotides, which specifically bind to a target molecule: they work as antibodies, but without many of the drawbacks associated to the use of antibodies. We propose to immobilize aptamers on scaffolds to retain specific proteins, acting as docking points to guide cell activity. In particular, we show the results obtained by enriching different polymeric scaffolds with aptamers against human fibronectin, a naturally abundant protein in tissues, which plays a pivotal role in cell adhesion. We demonstrate that scaffold enrichment with aptamers lead to a better colonization of the substrate from cells. The results we obtained pave the way to the possibility of further investigating the role of aptamers as useful molecules to improve scaffold biocompatibility in the contest of tissue engineering.

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Macroporous chitosan hydrogels: Effects of sulfur on the loading and release behaviour of amino acid-based compounds

Cited by 26 publications

References 33 publications

Ionically Crosslinked Thermoresponsive Chitosan Hydrogels formed In Situ: A Conceptual Basis for Deeper Understanding

Ionically Crosslinked Thermoresponsive Chitosan Hydrogels formed In Situ: A Conceptual Basis for Deeper Understanding

Surface modification of chitosan films with a fibronectin fragment–DNA aptamer complex to enhance osteoblastic cell activity: A mass spectrometry approach probing evidence on protein behavior

Aptamer-Mediated Selective Protein Affinity to Improve Scaffold Biocompatibility

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