Macrophages in Prostatic Fluid

Abstract

Non-specific esterase staining has been found useful in the detection of macrophages in prostatic fluid (PF). In the early stage of acute prostatitis most of the leucocytes in a smear of PF took up the stain, suggesting the dominant participation of macrophages in the inflammatory area in this stage of the disease. In chronic prostatitis, only a few leucocytes took up the stain. In female acute cystitis, none of the large numbers of leucocytes exhibited staining at any stage of the disease. The reason for this is still to be determined. Frozen sections of prostatic tissue from patients with chronic prostatitis revealed similar cells around the acini and these appeared to be the source of the macrophages which appeared in the PF.

Mise en évidence des cellules inflammatoires dans le sperme

“…Les macrophages ont 6t6 color6s sur frottis par la coloration de l'est6rase non sp6cifique [22,27] qui identifie 6galement les plasmocytes et les lymphocytes. Les frottis de sperme ont 6t6 fix6s pendant 10 minutes entre 4 et 10~ par une solution tampon h PH 6,6 d'ac6tone et de tbrmaline.…”

Immunocytological Analysis of Leukocyte Subpopulations in Urine Specimens before and after Prostatic Massage

“…Macrophages have been found to be the other most frequent cell type contributing with 11.1% to the total leukocyte count in VB 3. These cells have been suggested to play a role in acute prostatitis where they are found to be more numerous [5]. In nonbacterial prostatitis (now called inflammatory CPPS), they have been found to be more activated in the early than in the chronic stage [8].…”

“…Even those studies investigating macrophages in EPS refer to ‘leukocytes’ or ‘white blood cells’, because their methods of nonspecific staining do not allow further discrimination [5, 6, 7, 8]. Moreover, round cells of spermatogenesis in EPS may simulate leukocytic involvement [19].…”

“…However, we preferred a different stain for macrophages. Whereas Nishimura et al [5, 6, 8]applied a nonspecific esterase stain, we used a specific monoclonal antibody stain. On the other hand, our data are in good agreement with the results of Anderson and Weller [7]who counted 17% macrophages in the EPS of patients with chronic prostatitis by PAP stain.…”

“…Papanicolaou (PAP) stain as a routine procedure to quantify leukocytes in EPS or VB 3 is only capable of definitely identifying granulocytes. Using nonspecific esterase stain, fat–laden macrophages have been identified [5, 6], whereas others have tried to quantify macrophages in EPS by PAP stain [7]. Nishimura et al [8]analyzed the activation of macrophages in an in vitro experiment.…”