Macrophages in Drosophila embryos and L2 cells exhibit scavenger receptor-mediated endocytosis.

Abrams, John; Lux, Alison; Steller, Hermann; Krieger, Monty

doi:10.1073/pnas.89.21.10375

Cited by 90 publications

(53 citation statements)

References 28 publications

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“…We have shown that the shape of the dose-response curves for LDL and oxLDL induced MAPK activation was similar to the curve obtained for the binding/degradation of the radioiodinated lipoproteins. Although parameters obtained from degradation assays generally represent metabolism of the ligand at steady state rather than binding at equilibrium [33], the apparent dissociation constants determined at 37°C were found to be very similar to those determined for binding, uptake and degradation [34][35][36]. Hence, our data suggest a dose-response relationship of scavenger or apoB/E receptor binding/degradation and MAPK stimulation.…”

Section: Discussionmentioning

confidence: 59%

Stimulation of mitogen activated protein kinase by LDL and oxLDL in human U‐937 macrophage‐like cells

Deigner

Claus

1996

FEBS Letters

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Mitogen activated protein kinase in extracts of U-937 macrophage-like cells was stimulated by LDL and oxLDL. A maximum value (161% of the basal phosphotransferase activity) was obtained after 6 min exposure to oxidized LDL (27 Ixg/ml) using APRTPGGRR peptide substrate. The activatory effect was more pronounced (LDL 181%, oxLDL 201%) when MAPK of stimulated cells was immnnoprecipitated with anti-p42 MArK antibodies and phuspho~ransferuse activity was assayed in immune complexes. Stimulation produced by oxLDL was inhibited by poly I, fucoidan, dextran sulfate and by the MAPKK inhibitor PD 098059 but not by PMA-mediated depletion of PKC or by pre-treatment with chioroquine or with pertussis toxin. These results suggest a direct mitogenic effect of LDL which, in the case of oxLDL, is dependent on scavenger receptor llgation but not on G-protein mediated or PKCdependent signal transduction.

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Section: Discussionmentioning

confidence: 59%

Stimulation of mitogen activated protein kinase by LDL and oxLDL in human U‐937 macrophage‐like cells

Deigner

Claus

1996

FEBS Letters

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“…Candidates could include Toll-like receptors or SRs on hemocytes or other cell types, as it is already known that apoLp-III is taken up by granular hemocytes (22), and that Drosophila cells appear to exhibit SR binding of ␤-glucans and other pathogen-associated molecular patterns (PAMPs) and endocytosis of modified LDL (50,51). In mammals, bacterial lipoproteins bind the Toll-like receptor 2, which is itself a pattern recognition receptor (52,53).…”

Section: Discussionmentioning

confidence: 99%

A Novel Role for an Insect Apolipoprotein (Apolipophorin III) in β-1,3-Glucan Pattern Recognition and Cellular Encapsulation Reactions

Whitten

Tew

Lee

et al. 2004

The Journal of Immunology

203

163

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Lipoproteins and molecules for pattern recognition are centrally important in the innate immune response of both vertebrates and invertebrates. Mammalian apolipoproteins such as apolipoprotein E (apoE) are involved in LPS detoxification, phagocytosis, and possibly pattern recognition. The multifunctional insect protein, apolipophorin III (apoLp-III), is homologous to apoE. In this study we describe novel roles for apoLp-III in pattern recognition and multicellular encapsulation reactions in the innate immune response, which may be of direct relevance to mammalian systems. It is known that apoLp-III stimulates antimicrobial peptide production in insect blood, enhances phagocytosis by insect blood cells (hemocytes), and binds and detoxifies LPS and lipoteichoic acid. In the present study we show that apoLp-III from the greater wax moth, Galleria mellonella, also binds to fungal conidia and β-1,3-glucan and therefore may act as a pattern recognition molecule for multiple microbial and parasitic invaders. This protein also stimulates increases in cellular encapsulation of nonself particles by the blood cells and exerts shorter term, time-dependent, modulatory effects on cell attachment and spreading. All these responses are dose dependent, occur within physiological levels, and, with the notable exception of β-glucan binding, are only observed with the lipid-associated form of apoLp-III. Preliminary studies also established a beneficial role for apoLp-III in the in vivo response to an entomopathogenic fungus. These data suggest a wide range of immune functions for a multiple specificity pattern recognition molecule and may provide a useful model for identifying further potential roles for homologous proteins in mammalian immunology, particularly in terms of fungal infections, pneumoconiosis, and granulomatous reactions.

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“…8,25,26 However, not all Drosophila cell lines have endocytotic capability. 27 The fact that C6/36 cells are actively attracted to apoptotic cells and aggressively phagocytose fragmented apoptotic bodies suggests that this cell line, similar to the Drosophila S2 cell line, may have a mesoderm origin and display hemocyte/macrophage functionality.…”

Section: Resultsmentioning

confidence: 99%

Induction of reaper ortholog mx in mosquito midgut cells following baculovirus infection

et al. 2011

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Macrophages in Drosophila embryos and L2 cells exhibit scavenger receptor-mediated endocytosis.

Abstract: Mammalian macrophage scavenger recep-

Cited by 90 publications

References 28 publications

Stimulation of mitogen activated protein kinase by LDL and oxLDL in human U‐937 macrophage‐like cells

Stimulation of mitogen activated protein kinase by LDL and oxLDL in human U‐937 macrophage‐like cells

A Novel Role for an Insect Apolipoprotein (Apolipophorin III) in β-1,3-Glucan Pattern Recognition and Cellular Encapsulation Reactions

Induction of reaper ortholog mx in mosquito midgut cells following baculovirus infection

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